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Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... or the same volume of vehicle containing ghrelin receptor antagonist (GRA; [D-Lys3]-GHRP-6; 3.3mg/kg BW; Sigma-Aldrich G4535). This GRA dose was based on our previous report demonstrating its ability to restore cholecystokinin-induced NTS neuronal activation in food deprived rats [20] ...
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bioRxiv - Systems Biology 2022Quote: ... cells were washed with PBS and their nuclei were stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... After the addition of the appropriate amount of the 6× Laemmli Sample Buffer (Bioland scientific LLC, sab03-02) with 5% ß-mercaptoethanol (Sigma) protein samples (10-30 μg ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized (n=6) and treated with vehicle (10% D-a-tocopherol polyethylene glycol 1000 succinate in PBS, Sigma Aldrich) or VE822 (60 mg/kg ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Pancreas-specific Cre recombinase activity was induced in 6-week-old mice through the administration of tamoxifen (Sigma-Aldrich, cat. # T5648) daily for 6 consecutive days at a dose of 0.15 mg/g body weight followed by rest for a week before onset of ACP induction phase.
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... KD-LX2 and Scr-LX2 cells were seeded in 6-well-plates (4x104 cells/well) for 48hrs before being stained with Nile Red (Sigma-Aldrich) and evaluated with densitometry by ImageJ software to measure lipid content [24] ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Immunology 2024Quote: Bone marrow-derived macrophages were prepared from C57BL/6 and Rel-/-mice as described.68 Macrophages were activated on day 6 with lipid A for the time indicated in each experiment (Sigma-Aldrich). Nascent transcript RNA-seq was performed and the data were analyzed as described.68,69
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bioRxiv - Neuroscience 2024Quote: ... Slides containing the sections were immersed for 40 min in 10 mM citrate buffer pH 6 (sodium citrate, Sigma Aldrich, Germany) at 96°C in a water bath ...
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bioRxiv - Microbiology 2024Quote: ... approximately 7×105 cells growing on 6-well plates were washed with PBS and harvested with Tri Reagent (Sigma Cat#T9424). Total RNA was extracted ...
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bioRxiv - Cancer Biology 2024Quote: 0.5 to 1 million cells were plated into 6-well plate and treated with 0.5 or 1 mg/ml Dox (Sigma-Aldrich #D9891) to induce FGFR4 overexpression or knockdown for 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Neuroscience 2024Quote: Mice 4-6 weeks old were given three doses 150 mg/kg or 200 mg/kg (i.p) tamoxifen (TAM) (Sigma-Aldrich T5648) dissolved in peanut oil (20 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Cochlear explants were cultured for 6 days in DMEM with 10% fetal bovine serum and 10 µg/ml ciprofloxacin (Sigma, 17850). Explants were then fixed in 4% PFA for 15 min at room temperature before being processed for immunolabeling.
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bioRxiv - Plant Biology 2024Quote: Flowers at position 6 were gently incubated in staining solution (25 µM (2’,7’-dichlorodihydrofluorescein diacetate) (H2DCF-DA) (Sigma-Aldrich, D6883), 50 mM KCL ...
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bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... for differentiation induction 100.000 cells were plated in a 6-well plate and maintained in complete media supplemented with 20μM retinoic acid (Sigma-Aldrich, R2625) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: MSCs were seeded overnight on 24 or 6 well plates in normal α-MEM supplemented with 10% fetal bovine serum (FBS, Sigma), 2 mM glutamine ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... mice were fasted for 6 hours (for GTT) or 5 hours (for ITT) and received an intraperitoneal injection of glucose (1g/kg, Sigma Aldrich) or insulin (0.75 units/kg ...
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bioRxiv - Biophysics 2023Quote: Mechanically passaged hiPSCS were plated on 10 µg/mL Vitronectin XF in 6 well TC plates in 1 μM all-trans-RA (Cat. No. R2500-25MG, Sigma-Aldrich) to NutriStem® hPSC XF Medium (Growth Factor-Free)(Cat ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Immunology 2022Quote: 5–7-weeks old C57BL/6 mice bearing GL261 or CT-2A tumors were treated with KDM6B inhibitor (GSK-J4; Sigma-Aldrich); 1mg per mouse ...
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bioRxiv - Neuroscience 2023Quote: ... The spinal column was removed and 4-6 lumbar level DRG were dissected and placed onto coverslips coated with Poly-L-Lysine (Sigma, P8920). DRG were allowed to recover for 8 minutes (P4 ...
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bioRxiv - Neuroscience 2023Quote: ... Western Blotting experiments on viral particles were carried out on the collected culture media concentrated with Vivaspin 6 MWCO 30 kDa membranes (Sigma Aldrich). Samples were then reduced and denatured by boiling in 4X Laemmli sample buffer at 95°C for 5 minutes ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes were differentiated into THP1-derived macrophages by stimulating 5×105 THP-1 monocytes/well in 6-well plate with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) (#P8139, Sigma Aldrich) for 48 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The incubation was performed in the presence and absence of an NADPH generating system: 1 mM glucose-6-phosphate (Sigma Aldrich) 0.1 mM NADP+ (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 16uL of 10000 units mL-1 (approximately 6 mg mL-1) penicillin G and 10 mg ml-1 streptomycin solution (Sigma-Aldrich) was added to each tray to inhibit the growth of pathogens (Gangur & Marshall 2020) ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Neuroscience 2023Quote: ... cell colonies were grown on Matrigel-coated 6-well plates (Matrigel: Cat No. 354277, Corning; Plates: Cat No. CLS3516-50EA, Sigma Aldrich) in mTeSR Plus for at least three passages before being dissociated for downstream differentiation protocols ...
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bioRxiv - Immunology 2023Quote: ... IDE12 cells were also grown to confluency in T25 flasks and either seeded at 1x106 cells/well in 6-well plates (Millipore Sigma) or 3x105 cells/well in 24-well plates (Corning) ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... motoneurons grown for 6 DIV on laminin-111-coated glass coverslips were treated with 10 µg/mL puromycin (P8833, Sigma-Aldrich) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed with PBST and incubated at room temperature for 2 h with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) diluted in PBST ...
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bioRxiv - Plant Biology 2023Quote: ... followed by wash with 100% ethanol twice and sown on 1X MS medium (PT011, HiMedia Laboratories, India) containing 0.025% ethanol (Mock) or 6 μM dexamethasone (DEX) (D4902, Sigma-Aldrich, USA). After sowing ...
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bioRxiv - Neuroscience 2023Quote: Induced microglia-like cells were treated for 6 and 24h with 100ng/ml of LPS from Escherichia coli (E. coli) O55:B5 (Sigma L2880) that was diluted first in DPBS and then added to the iMG media ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole, 10 μg/ml, Cat.No.: D27802, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: Nuclear DNA integrity and gross cell morphology in dPPM was assessed with 4’,6’- diamidino - 2 - phenyl indole (DAPI, Sigma-Aldrich) staining and fluorescence microscopy (Nikon Eclipse Ti ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... the SCG neurons of postnatal day 1–2 NMRI strain mice or DRG neurons from E16 embryos were grown 6 DIV on poly-L-ornithine-laminin (P3655 and CC095, Sigma-Aldrich) coated dishes with 30 ng/ml of mouse NGF (G5141 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thrice with 1×PBS and incubated for 15 min at RT with 0.1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, Poland). After a final three washes with 1×PBS ...