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Citations for Millipore Sigma :
7601 - 7650 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Human melanocytes seeded at 1.0×105 – 2.0×105 cells/well density in 6-well plates were incubated in viral supernatant with 10µg/mL polybrene (Sigma, TR-1003) and centrifuged at 300xg for 60 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mitochondria were incubated with 0.2 μM TMRE for 15 minutes and then measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Neuroscience 2020Quote: ... was used as a positive control and incubated simultaneously with MitoSOX Red indicator at a concentration of 1.25 μM and afterwards measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... A549 cells were then infected by culture-medium-diluted viral supernatants in the presence of 6 μg/ml polybrene (Sigma #H9268) in 3 consecutive days and selected with puromycin (1.5 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with DAPI (4’,6’-diamidino-2-phénylindole) and slides mounted with Fluoromount Aqueous Mounting Medium (F4680, Sigma Aldrich, France) before analysis using Zeiss LSM microscope confocal.
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bioRxiv - Cell Biology 2021Quote: ... collagen-grown cells were collected and incubated with ITGA-6 neutralizing antibody (Merck Millipore #MAB1378, clone NKI-GoH3 at 40ug/ml) for 60 mins at 37 C before plating on hydrogels ...
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...
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bioRxiv - Molecular Biology 2022Quote: ... dissociated monolayer cells were seeded inside a 50uL Matrigel droplet on a 6-well clear polystyrene flat-bottom plate (Millipore Sigma) at a density of 250 cell/uL Matrigel in the indicated media conditions + 1uM 4OHT or an equivalent volume of DMSO at the time of seeding ...
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bioRxiv - Microbiology 2022Quote: ... For verification of the expected product size and RNA integrity, RNA gels (6 % PAA, 7 M urea) were prepared with subsequent staining using StainsAll (Sigma-Aldrich).
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were supplemented with 500 µL antibiotic-free media and cultured for up to 6 days in RPMI 1640 media (Sigma-Aldrich). At each time point ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich) for one hour ...
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bioRxiv - Neuroscience 2020Quote: ... Behaviour was monitored at 500 fps in 6 dpf larvae with their head restrained in 2% low-melting point agarose (Sigma-Aldrich) and their tail free to move ...
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bioRxiv - Immunology 2020Quote: ... Sorted cells were incubated with slices (5×105 cells/slice) for 6 hours in Hanks’ Balanced Salt solution (Sigma-Aldrich, USA) supplemented with 1% of penicillin/streptomycin solution (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ciprofloxacin hydrochloride monohydrate (stock solutions prepared in DI water) and amoxicillin (stock solution prepared in phosphate buffer, pH 6) were purchased from Sigma-Aldrich. Solutions of antibiotics in media were prepared shortly before the experiments ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... crystals were rapidly passed through a drop of fluorinated oil (Fomblin® Y LVAC 14/6, average MW 2,500 from Sigma Aldrich) to prevent ice formation and directly frozen on the beamline in the nitrogen stream at 100 K ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were sonicated for two times ten secs at 50% amplitude on ice and incubated for 30 min at RT in lysis buffer consisting of 6 M urea (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... age-matched male Tg2576 mice were assigned to receive 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, Sigma-Aldrich, St. Louis, MO) by intraperitoneal injection for 1 week ...
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bioRxiv - Immunology 2020Quote: ... Then the slips were washed with PBS for three times, and after that the hemocytes were stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/ml) (Sigma, USA) for 5 min [55] ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 0.1 M sodium cacodylate buffer containing 1% OsO4 and 1.5% K4Fe(CN)6 (Sigma-Aldrich, St. Louis, MO), en bloc stained with 2% aqueous uranyl acetate ...
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bioRxiv - Evolutionary Biology 2021Quote: Total bacterial counts were determined by plating out 25 µl of a dilution series ranging from 10−1 to 10−6 on LB-agar plates (Sigma-Aldrich). To identify recipient strains (carrying the dTomato marker ...
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bioRxiv - Microbiology 2021Quote: ... cells from control and experimental culture were washed with PBS and mounted with DAPI (4’,6-diamidino-2-phenylindole) over glass slides using slide mounting media from Sigma-Aldrich and observed under the fluorescent microscope ...
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bioRxiv - Biochemistry 2021Quote: ... cells were fixed using a 6% (v/v) formaldehyde:phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged after reaching 80% confluency by washing with PBS (Dulbecco’s Phosphate Buffered Saline, Sigma-Aldrich, Cat. #D8537-6×500ML) and incubating in 1 X trypsin-EDTA (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were plated on polyester membrane inserts (0.4 μm pore) in 6 well culture plates (Corning Costar CLS3450-24EA, Sigma-Aldrich, UK), 2-3 slices per insert ...
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bioRxiv - Neuroscience 2022Quote: ... The permeability of the cupular matrix was measured by recording the penetration of a negatively-charged fluorophore (6-carboxyfluorescein; Sigma-Aldrich) into the cupula ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genetics 2022Quote: 750k low-passage 293FT cells were seeded into each well of a 6-well plate coated with poly-L-ornithine (Sigma, P2533). One day after seeding ...
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bioRxiv - Neuroscience 2022Quote: ... CAS# 614-96-0), 6-methylindole (97%, CAS# 3420-02-8) and 7-methylindole (97%, CAS# 933-67-5) were acquired from Sigma-Aldrich, and 4-methylindole (99% ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Physiology 2019Quote: ... Cells were then incubated with activator Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA; Sigma, Schnelldorf, Germany; 0.1 μM; 6 h) with or without 1 μM PKC inhibitors Calphostin C (Tocris) ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature in the dark and incubated with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich) for 10 min ...
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bioRxiv - Cell Biology 2019Quote: Confluent HeLa cells grown in 6- or 12-well plates were treated with deferiprone (DFP; 379409, Sigma-Aldrich; working concentration, 1mM) for the time durations as indicated.
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bioRxiv - Physiology 2019Quote: Approximately 15 mg of the left TA muscle was homogenized using a glass tissue-homogenizer in 1.5 ml ice-cold lysis buffer (6 M Urea, 2 M Thiourea, 10 mM DTT, Complete Protease Inhibitor (1 tablet per 10 ml lysis, Sigma-Aldrich), PhosSTOP (2 tablet per 10 ml lysis ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Immunology 2020Quote: ... miR-511-3p transfected cells as well as controls were stimulated on day 6 with RSG in the presence or absence of LPS (Sigma Aldrich) for 24 hours before harvesting ...
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bioRxiv - Molecular Biology 2020Quote: For lentiviral transduction 200.000-250.000 ES cells per well were seeded on gelatinized 6-well dishes in ES medium containing 8 μg/ml polybrene (Sigma, #H9268). Viral supernatant was added to the cells and plates were spun in a prewarmed centrifuge (Heraeus Multifuge 4KR ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then thoroughly washed (6 × 10 min) with PBS-T before revealed with SigmaFast NBT/BCIP substrate solution (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... The remaining 2 ml were added to falcon tubes containing 4 ml of 6 M GndHCl (Sigma Aldrich, Saint Louis, Missouri) and mixed thoroughly to denature the samples ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl PCR product for each barcoded sample was mixed with 5 μl 6×Orange G DNA Loading Dye (Orange G, 6X, Sigma, Germany) and run on a 1 % agarose gel in TAE 1X buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed again for three times before the final staining of the nuclei using 4’,6-diamidin-2-phenylindol (DAPI, Sigma-Aldrich). Afterwards ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.