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Citations for Millipore Sigma :
7551 - 7600 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Protein extracts were prepared in RIPA buffer (R0278, Sigma) with protease inhibitor 1X (ab270055 ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein expression was induced with 0.2 mM IPTG (Sigma) final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were mixed with DTT (#D9779-10G, Sigma) and loading sample buffer (#NP0007 ...
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bioRxiv - Immunology 2024Quote: ... A protein A-Sepharose column (Sigma #ge17-1279-03) was used for mAb purification ...
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bioRxiv - Microbiology 2024Quote: ... We also employed SYPRO Orange Protein stain (Millipore Sigma), a protein dye ...
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bioRxiv - Genomics 2022Quote: ... The tissue was gently Dounce homogenized in 500 µL of Nuclei Isolation Buffer (Sigma Nuclei PURE Prep Kit: Lysis Buffer, 1 mM DTT, Sigma Nuclei PURE Prep Kit: 10% Triton X-100). After a brief centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... were maintained at 37°C on rat collagen diluted at 100µg/mL in water (Cultrex; catalog no. 3443-100-01) in EndoGro medium (Merck Millipore; catalog no. SCME004) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 14 °C (a temperature not permissible for recycling) with unconjugated goat anti-rat IgG (Sigma, 4 μg/ml) to block labelled channels remaining at the cell surface ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated with the primary antibody (rat anti-serotonin, Chemicon MAB352, 5/1000; rabbit anti-tyrosine hydroxylase, Sigma Aldrich SAB2701683, 2/1000) in buffer (1% sheep serum ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Female rats were administered an intraperitoneal injection of “Hellabrunner Mischung” comprising ketamine hydrochloride/xylazine hydrochloride solution (1ml/kg, Sigma Aldrich, Taufkirchen, Germany). The surgical procedure followed the description of Spicer and colleagues (44) ...
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bioRxiv - Physiology 2020Quote: DN was induced experimentally in 12-h fasting rats except for the normal group through a single intraperitoneal injection of 0.5 mL STZ (Sigma-Aldrich, Deisenhofen, Germany) at a dosage of 60 mg/kg of body weight ...
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bioRxiv - Neuroscience 2021Quote: ... (2) rats underwent UVN and received intraperitoneally one L-thyroxine (L-T4) injection (10 µg/kg, dissolved in saline, Sigma, Cat#T2501) at the end of the surgery and one injection/day during the first 3 days post-lesion (UVN-T4 group ...
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bioRxiv - Pathology 2024Quote: ... The diabetic rats in the DM+GR group were given a standard diet and were also given glyburide (Sigma-Aldrich; Merk KGaA) at 4 mg/kg in 0.5 ml of 0.5% Tween 80 solution as a supplement ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher], and anti-NeuN [Millipore Sigma] ...
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bioRxiv - Immunology 2023Quote: ... and subsequently incubated with anti-CD16/CD32 (eBioscience, Cat #14-0161-86, clone 93) and 10% rat-IgG1 (Sigma-Aldrich, Cat #I8015) in PBS containing 0.1% BSA (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysates (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-HKU1 proteins or lacking the fusion protein (Δ Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland). HAE cultures were washed thrice with 100 μl of 1 × PBS and subsequently inoculated with S-HKU1 or VSV-G pseudoviruses ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysate (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The phosphatase assay was carried out using the Ser/Thr Phosphatase Assay Kit 1 (Millipore Sigma (MA, USA)) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Nuclear and cytoplasmic extracts were prepared from differentiated THP-1 cells using Nuclei EZ Prep kit (Sigma-NUC101) both from un-stimulated and MprA stimulated cells ...
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bioRxiv - Genetics 2020Quote: Cells were crosslinked with 1% formaldehyde for 10min before chromatin fragmentation with EZ-enzyme kit 17-375 (Millipore) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of the supernatant was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of each sample was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from 1 million cells in triplicates using GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Biochemistry 2019Quote: ... Ca2+ transport was terminated at serial time intervals by vacuum filtering 100 µL of assay solution (1 µg of SR protein) through a glass-fiber filter (Millipore HA, with 0.45 µm pore size), which was washed twice with 5 mL of ice-cold 150 mM NaCl solution ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Cell Biology 2024Quote: ... For immunoprecipitation PNS were incubated with Anti-V5 Agarose Affinity Gel conjugated beads or with Protein-A beads (Sigma, 1:10 w/v swollen in PBS) and anti-HA antibody for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were transferred onto 0.2 μm pore size Protran nitrocellulose membranes (Sigma-Aldrich, St. Louis, MO) followed by western blot for GP1 using a rabbit polyclonal sera recognizing a peptide (TKRWGFRSGVPPKVV ...
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bioRxiv - Biophysics 2021Quote: ... Samples were applied to a SDS-PAGE and transferred to an Immobilon PVDF membrane (Millipore, USA). The membranes were blocked with 5% skimmed milk prepared using Tris-buffered saline with 0.05% Tween 20 ...
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bioRxiv - Biophysics 2019Quote: ... cell membranes containing TRPA1 was incubated with 100 µM iodoacetamide (IA) or BODIPY-IA(Millipore Sigma) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Ten percent polyacrylamide gels were used to clarify lysates before transferring onto PVDF membranes (Millipore, IPVH00010) at 90 V for 90 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo, Millipore, #WBLUR0100) for chemiluminescence detection using ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated and non-biotinylated lysates were then washed in a 10kDa membrane cutoff filter (Sigma) with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were subsequently separated by SDS-PAGE and transferred to Immobilon-P polyvinylidene difluoride membranes (Millipore). Immunoblot analysis was performed with the antibodies indicated and visualization was achieved with the Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... 12.5 or 15% polyacrylamide gel and transferred to PVDF membrane with 0.45 μm pore size (Millipore). The membranes were blocked with 5% skim milk in TBS supplemented with 0.1% Tween 20 (TBS-T ...
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bioRxiv - Biophysics 2022Quote: ... Membranes were washed with PBST three times and developed with an ECL-HRP (Cat# WBULS0100, Millipore) system ...
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bioRxiv - Plant Biology 2022Quote: ... The solution was again filtered through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). The Fe content was analysed using ICP-OES (iCAP 6300 Duo ICP-OES ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... nitrocellulose membrane (Bio–Rad, USA) and immobilon™ western chemiluminescent HRP substrate (Merck, Millipore, United States); streptozotocin (Enzo Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Following 3 x TBS-T washes the membranes were developed with Immunoblot Forte Western HRP (Millipore), according to the manufacturer’s instructions and imaged using an Amersham™ Imager 600 (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were developed with enhanced chemiluminescent reagent (Immobilon Western HRP substrate and Luminol reagent (WBKLS0500, Millipore) using a Fujifilm LAS3000 imaging system ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was washed in 1x TBST and then incubated with Forte HRP substrate (Millipore WBLUF0100) followed by imaging on ImageQuant LAS 4000 mini biomolecular imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were blocked in 4% milk for 20 minutes and were probed with anti-FLAG (Sigma) for 4 hours at room temperature or overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The remainder of the culture was poured twice over a polyvinylidene fluoride membrane filter (Merck Millipore) with a pore size of 5 µm ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was blocked with 5% milk in 0.1% PBS-Tween 20 (PBS-T) (Sigma-Aldrich), incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... This medium was sterilized by filtration through a 0.45 μm nitrate-cellulose membrane (Millipore, Molsheim, France). Phenotypes were measured in a medium containing 260 g.L-1 of sugars (50% fructose-50% glucose ...
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bioRxiv - Developmental Biology 2019Quote: ... cells and Transwell membranes containing protrusions were permeabilised in PBS containing 0.2-0.5 % Triton-X100 (Sigma), blocked in 4 % goat serum (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were separated by a 10% polyacrylamide gel and transferred to a PVDF membrane (Millipore). The membrane was blocked with the Can Get Signal / PVDF blocking reagent (Toyobo ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were prepared by painting a solution of soybean asolectin (10 mg/ml in decane, Sigma) across a 250 μm-diameter hole on a Teflon partition separating two compartments filled with a recording solution (150 mM KCl ...