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Citations for Millipore Sigma :
7751 - 7800 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... These are then gently transferred to a 6-well dish containing either a membrane insert (Millipore) or a poly-L-lysine coated coverslip (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2024Quote: ... China) for separation and then transferred to a 0.2 μm PVDF membrane (Cat# 03010040001, Millipore, Germany). The PVDF membrane was blocked in TBST solution containing 5% milk powder and then incubated overnight at 4°C with diluted primary antibodies (5% milk powder TBST) ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were developed using Fusion Fx (Vilber Lourmat) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: ... 20% (vol/vol) methanol as transfer buffer to PVDF membranes (0.45 μm; cat. no. IPFL00010; Millipore), preactivated in pure methanol ...
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bioRxiv - Microbiology 2022Quote: ... or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). Normalised DNA (at a concentration of 0.2 ng/ml ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... dark agouti rats via a single intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, in trisodium citrate buffer, pH 4.5, Sigma-Aldrich Co, MO, USA), with control animals receiving an equivalent volume of vehicle ...
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bioRxiv - Neuroscience 2021Quote: Sprague Dawley male rats received four ip injections of 4 mg/kg methamphetamine hydrocholoride (dissolved in 0.9% saline Sigma, St Louis, MO, USA) every two hours for 8 hours with rectal probe body temperature measurements every hour ...
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bioRxiv - Neuroscience 2020Quote: ... Rat pups received oral administration of fluoxetine (10 mg/kg; kind gift from IPCA Laboratories) or vehicle (5% sucrose, Sigma-Aldrich, Cat no.) once daily through a micropipette (0.5-10 μL ...
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bioRxiv - Physiology 2021Quote: ... Cell pellets were resuspended in growth medium and plated in tissue culture dishes coated with type I rat tail collagen (Sigma, St. Louis, MO). Cultures were expanded and frozen at passage 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The tube was kept at 37 °C for 10 min before a 13 μL aliquot of the Sprague-Dawley rat liver microsome (Sigma-Aldrich, No. M9066) was added ...
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bioRxiv - Bioengineering 2020Quote: The effect of HMGB1 on inducing the migration of rat BMSCs were examined using a Trans-well plate (Millipo Sigma, St Louise, USA). The rat BMSCs in DMEM at passage 1 were seeded into the upper layer of insert well of the trans-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
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bioRxiv - Genomics 2023Quote: ... Rats received a single oral gavage administration of freshly prepared 40 mg/kg N-ethyl-N-nitrosourea (ENU) (Sigma Aldrich, St. Louis, MO) dissolved in PBS (Nova-Tech ...
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bioRxiv - Biophysics 2024Quote: ... measurements of the identified ChRs were expressed in a mouse neuroblastoma and rat neuron hybrid cell line (ND7/23 cells, ECACC 92090903, Sigma-Aldrich, Munich, Germany). Cells were cultured as previously described (Grimm et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... The elutes were incubated with S-protein beads (EMD Millipore) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: Protein samples were diluted in PBS pH=7.4 (Sigma-Aldrich). Neutravidin (NAv-Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were alkylated using 2-chloroacetamide (C0267-100G, Sigma-Aldrich) in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore, AmiconUltra 4 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were incubated with protein G agarose (IP04, Millipore), Flag affinity agaros (A2220 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were reduced with 100 mM DTT (Sigma, D9163) for 35 min at 57°C and then handled according to the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced with 10 mM dithiothreitol (Sigma Aldrich, UK) in ammonium bicarbonate for 30 min at 37°C and alkylated with 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were precleared with protein A-Sepharose beads (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... carrier protein and 0.2 mL of 100% Trichloroacetic acid (Sigma) was added to the extracted protein fraction ...
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bioRxiv - Cell Biology 2020Quote: ... protein aliquots were incubated with 10μg of free avidin (Sigma) per 1mg of input protein for 1h at 4°C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Buffer proteins were purchased from Sigma-Aldrich (St. Louis, MO), and salts were purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2021Quote: ... Protein bands were detected with either anti-Flag (Sigma-Aldrich) or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Mpro proteins were concentrated to 25 mg/ml by Millipore’s ultrafiltration columns and a final concentration of 1 mM DTT was added and stored at −80°C.
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bioRxiv - Developmental Biology 2021Quote: For Hyaluronic Acid-Binding Protein (HA-BP, Sigma-Aldrich, 385911) immunofluorescence ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Protein expression was induced with 0.1% L-arabinose (Sigma-Aldrich). Expression took place for 16 h at 15°C and 220 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Systems Biology 2020Quote: ... blots were stained with Ponseau S total protein stain (Sigma) prior to blocking at room temperature (5% skim milk ...
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bioRxiv - Developmental Biology 2021Quote: ... The filtrates were then concentrated in protein concentration columns (Millipore CENTRICON YM-10 Centrifugal Filter Unit 2 mL 10 kDa ...
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bioRxiv - Biochemistry 2021Quote: Protein was labeled with Fluorescein Isothiocyanate (FITC, Merk, Sigma-Aldrich) according to the supplier’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were precipitated with TCA (Sigma Aldrich, Cat# T0699) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fusion proteins were captured using V5 agarose beads (Sigma # A7345) that were pre-cleared overnight with PBS + 0.1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-cleared with 40 μl Protein A agarose beads (Millipore), and then immunoprecipitated with specific antibodies against REST/NRSF (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... the proteins were concentrated with Amicon Ultra 4 MWCO10kDa (Millipore) to approximately 3 – 4 mL ...
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bioRxiv - Molecular Biology 2019Quote: The protein was concentrated with Amicon Ultra 4 MWCO30kDa (Millipore) at 20 °C at 1650 × g ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were reduced with 2μl of 0.2M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... lysed in BugBuster Primary Amine Free Protein Extraction Reagent (Novagen) supplemented with recombinant lysozyme ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein complexes were eluted with 3xFlag peptide (200ug/mL, Sigma) in 5 column volumes Buffer 2 for 1 hour at 4C ...
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bioRxiv - Biophysics 2019Quote: ... then bound proteins were eluted with 3 mM desthiobiotin (Sigma) or 50 mM D-biotin (CHEM-IMPEX ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich). Equal amounts of protein lysates were separated by SDS-PAGE and proteins were transferred to nitrocellulose filters ...
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bioRxiv - Cell Biology 2019Quote: ... Relative protein loading was visualized using Ponceau S Solution (Sigma). Membranes were subsequently washed in TBST and blocked for 1 hour in 5% skim milk in TBST at RT ...