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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to Immobilon-FL PVDF (Millipore IPFL00010) in transfer buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2022Quote: ... A four-molar excess of scaffold protein (MSP1E3D1, Sigma) was added to the protein-lipid mix ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were probed with antibodies against FLAG M2 (Sigma), Cdc2 (Santa Cruz Biotechnologies SC-53217) ...
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bioRxiv - Genomics 2022Quote: ... bead-bound proteins were eluted in Laemmli buffer (Sigma) and resolved on a 4–20% SDS-polyacrylamide gel (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 30 μl of protein A agarose beads (Sigma). Samples were subjected to SDS-PAGE and immunoblot analysis as described (16,20,23,82,86–87) ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted proteins were precipitated with trichloroacetic acid (Sigma) and subject to MS analyses as described previously 13.
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bioRxiv - Microbiology 2023Quote: ... and proteins were eluted in 2X Laemmli (Sigma-Aldrich) or deglycosylated by an 1h treatment with PNGase F (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Neuroscience 2022Quote: ... Pelleted proteins were resuspended in 0.1 M NaOH (Sigma) and assayed for protein quantification using the Bradford assay (Biorad).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by Bradford assay (Sigma, #B6916), after which samples were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) final concentration at 95°C for 10 minutes and alkylated with iodoacetamide (Pierce,40 mM final 30 minutes) ...
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bioRxiv - Neuroscience 2022Quote: ... 30ul of equilibrated Protein G – agarose beads (Sigma 15920010) were added to the lysates and incubated further for 3 hours.
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bioRxiv - Neuroscience 2022Quote: ... and proteins were extracted in RIPA buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein synthesis inhibitor cycloheximide was purchased from Sigma-Aldrich. USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was concentrated using Amicon Ultracel-30K (Millipore) centrifuge filters and subsequently applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was digested with sequencing grade trypsin (Sigma; #T6567) and sequencing grade LysC (Wako ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to methanol activated PVDF (Millipore) membranes (350 mA for 60 minutes) ...
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bioRxiv - Molecular Biology 2024Quote: ... A mixture of Protein A agarose (Millipore, 16-157) and Protein G agarose (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated in a 3 kDa concentrator (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The proteins were transferred to Immobilon-FL (Millipore IPFL00010).
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bioRxiv - Biochemistry 2024Quote: ... proteins were expressed in BL21 (DE3)T1R cells (Sigma) at 18 °C overnight after induction with 1 mM IPTG ...
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bioRxiv - Biophysics 2024Quote: ... the proteins were concentrated using 30kDa MWCO Amicon (Millipore) ultra-centrifugal filters to 10 mg/mL and immediately injected in gel filtration column S200 10/300 (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µL of the Protein G agarose bead (Millipore) slurry was washed and added to the antibody-lysate mixture and incubated for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein G beads (Millipore, Cat. #16-266) to the immuno-complex for 2h ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
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bioRxiv - Molecular Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich) for 30 min at 37 °C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Genetics 2024Quote: ... Co-purifying proteins were imaged with anti-HA (Sigma) and anti-VSV (Bethyl Laboratories ...
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bioRxiv - Immunology 2024Quote: ... 20 μg of Ovalbumin recombinant protein (Sigma-Aldrich, A5503) (1:1 mixed with Alhydrogel ...
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bioRxiv - Biochemistry 2024Quote: ... protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chlorophorm phase separation ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ReadyBlueTM protein stain were purchased from Sigma-Aldrich. PUREfrex® 1.0 Solutions II (enzymes) ...
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bioRxiv - Developmental Biology 2023Quote: ... Ezview Red Protein G Affinity Gel (Sigma-Aldrich, E3403) was used for negative control samples ...
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bioRxiv - Genomics 2023Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) at 95°C for 10 min and alkylated for 30 min with 40 mM iodoacetamide (Pierce) ...
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bioRxiv - Immunology 2023Quote: ... filtered through 0.45 μm low- protein-binding filters (Millipore) and concentrated via 16 hours centrifugation at 3000 x g at 10 °C ...
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bioRxiv - Genomics 2022Quote: ... bound to Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Co-purification was investigated by western blot with an anti-GFP antibody (Roche #11814460001 ...
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bioRxiv - Biophysics 2022Quote: All Proteins and fluorophores were procured from Sigma-Aldrich and concentration was measured using Shimazdu uv-1900 spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... levels were normalized to total protein levels (Sigma-Aldrich, #E3138 ...
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bioRxiv - Microbiology 2022Quote: ... Samples were precleared with Protein A agarose beads (Millipore), immunoprecipitated with anti-REV-ERBα (NRIDI ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37ÅãC and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 60µL of Protein A beads (EMD Millipore, 16-156) were washed in IP lysis buffer then blocked for 1hour in 1%BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with ReadyBlue Protein Gel Stain (Sigma RSB-1L) overnight for Coomassie staining ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were dimethylated by adding 30 mM formaldehyde (Sigma) and 30 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... After a preclearing step with Protein G Sepharose (Sigma), equal amounts of proteins were then incubated with anti-Flag Beads (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...