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Citations for Millipore Sigma :
7551 - 7600 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... α-cell/ β-cell ratio was calculated following staining with antibodies against insulin (as above) and glucagon (primary antibody: mouse anti-glucagon 1:2000; Sigma-Aldrich, G2645) (secondary antibody goat anti-mouse Alexa Fluor 488 1:500 ...
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bioRxiv - Cancer Biology 2020Quote: Two biological replicates for each knock-down/rescue sample were analyzed by CUT&RUN using the anti-FLAG M2 mouse antibody (Sigma F1804-200UG) as described and sequenced with the Illumina HiSeq4000 (32) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
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bioRxiv - Immunology 2022Quote: Formalin-fixed and paraffin-embedded lung sections from mock and IAV-infected mice were prepared as previously described (47) and probed for epithelial or endothelial cells detection using monoclonal mouse anti-αSMA (1:10,000; A2547; Sigma-Aldrich, Darmstadt, Germany), rabbit anti-mouse CD31 (1:50 ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Cell Biology 2022Quote: ... free floating sections were incubated with mouse anti-FLAG clone M2 antibodies (1:2000 in the donkey serum blocking solution, Sigma-Aldrich F1804), overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibodies and their dilution rates used for immunostaining in this study are as follows: mouse monoclonal anti-GFAP (MAB360; Millipore, Billerica, MA) at 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: The following reagents were purchased from commercial sources: mouse monoclonal anti-dinitrophenol (DNP) IgE and thapsigargin were bought from Sigma-Aldrich (USA). DNP conjugated bovine serum albumin (DNP-BSA ...
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bioRxiv - Neuroscience 2020Quote: ... and then nuclei were simultaneously incubated with the three fluorescently-conjugated primary antibodies (0.1% BSA/1X PBS) for one hour at 4°C on rotation: mouse anti-NEUN-AF555 (Millipore, MAB377A5, 1:1000); mouse anti-PAX6-APC (Novus Biologicals ...
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bioRxiv - Immunology 2020Quote: ... The input and elution samples were analyzed by Western blot by probing with mouse anti-FLAG M2 antibody (Sigma Aldrich; 1:2000), rabbit anti-His tag antibody (Cell signaling ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibody 1:12000 mouse anti-α-Tubulin antibody (SIGMA, Cat. # T5168). Followed by 1h incubation with secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) followed by incubation with antibodies specific for GFP (mouse monoclonal anti-GFP [7.1 and 13.1] (Sigma-Aldrich Cat# 11814460001, RRID:AB_390913)) and Tub1 (rat monoclonal anti-tubulin alpha ...
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bioRxiv - Immunology 2021Quote: Plasma antibody levels of K11 and C9-tagged 5L7 were measured by ELISA using plates coated overnight at 2 μg/mL with either purified SIVmac239 gp140 FT or mouse anti- rhodopsin (C9) monoclonal Ab (EMD Millipore, 6B9575), respectively ...
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bioRxiv - Immunology 2020Quote: ... 5 μm sections of frozen post-ISOPROTERENOL hearts were stained with goat anti-mouse IgG-FITC (cat. F5387, Sigma-Aldrich, Dorset, UK), rat anti-mouse IgM-PE (cat ...
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bioRxiv - Plant Biology 2021Quote: ... Immunodetection was performed by incubating the immunoblot for 2 h with anti-mouse IgG conjugated with horseradish peroxidase (1:10,000 dilution; Millipore; Catalog # 12-349). Detection was performed using the SuperSignal West Dura Detection Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) for 4 hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the Dynabeads were resuspended in 100 μL of RIP wash buffer and 2.5 μL (2.5 μg) of mouse anti-FLAG (Sigma, clone M2, #F1804-1MG) antibody or isotype-matched control IgG1 (Cell Signaling #5415S ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Neuroscience 2022Quote: For immunocytochemical staining we used the following primary and secondary antibodies: mouse anti-NFL antibody clone DA2 (Merck Millipore, cat. no. MAB1615), mouse anti-NFL antibody clone F-12 (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2019Quote: ... MA) and the secondary antibody used was HRP-conjugated goat polyclonal anti-mouse IgA (1:500; A4789; Sigma Aldrich; St. Louis, MO). HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used at 1:1000 and included: mouse monoclonal anti-β1/β2-adaptins (clone 100/1; Sigma, St. Louis, MO); mouse monoclonal anti-clathrin heavy chain (clone 23 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Cancer Biology 2019Quote: ... Equivalent amounts of DNA were spotted on a Nylon membrane using a dot blot apparatus (Cleaver Scientific) and probed with mouse anti-TOP1cc (EMD Millipore, MABE1084). Ethidium bromide staining of spotted DNA served as loading controls ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Microbiology 2020Quote: Secondary antibodies used are: Goat anti-mouse IgG (H+L) highly cross-adsorbed Alexa fluor plus 647 secondary antibody (Sigma-Aldrich, A32728), Goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Systems Biology 2020Quote: ... Resolved proteins were transferred to nitrocellulose membranes and detected by standard Western blotting using monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and HRP labelled anti-mouse IgG secondary antibody (Cell Signalling technology ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-calbindin (1:1000, mouse, Sigma Aldrich, #C9848). After 3 rinses with PBS for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to primary antibody solution containing monoclonal mouse anti-vesicular glutamate transporter 1 (VGlut1) antibody (1:750; Millipore, Burlington, MA) in blocking buffer and incubated overnight at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... nuclei were incubated with blocking solution (10% goat serum in PBS) for 30 min at 37°C and stained with mouse-anti-γH2AX (1/500, Millipore 05-636) for 1 hr at 37°C followed by O/N incubation at 4°C ...
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bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed again 5 times with the washing buffer to discard unbound serum and 100 μl of horseradish peroxidase-conjugated goat anti-mouse IgG (Sigma-Aldrich, USA) diluted at 1:2000 in 1% BSA in washing buffer was added to each well followed by 1 hour incubation at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilized for one hour with 0.5 % Triton X-100 and 6 % FBS in PBS and further incubated for another hour with mouse anti-FLAG M2 (1:500; Sigma-Aldrich F1803) in PBS with 0.1 % Triton X-100 and 6 % FBS (room temperature) ...
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bioRxiv - Cancer Biology 2021Quote: ... α tubulin was used as a loading control and the probing was done by using mouse monoclonal anti-α-tubulin (Sigma T5168). The secondary antibody used was goat anti-mouse (HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) or 5ug Normal Mouse IgG Polyclonal Antibody (MERCK ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were analysed using 4-12% Bis-Tris polyacrylamide gels in MOPS buffer followed by Coomassie staining or western blot using the following antibodies: Mouse anti-FLAG M2 (Sigma, 1:10,000). RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated 18 hours at 4°C with mouse anti-TH (1/5000, MAB318, Sigma-Aldrich, Saint Louis, MO, USA) in blocking solution ...
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bioRxiv - Developmental Biology 2022Quote: ... and cultures were incubated overnight at 4°C with primary antibodies diluted in TBS/10% goat serum: mouse anti-β-tubulin-III (Sigma #T8660) or mouse anti-MAP2 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore, MAB1574, 1:5,000).
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bioRxiv - Neuroscience 2022Quote: ... Larvae were washed three times in 0.1% PBST and incubated with secondary antibodies: anti-mouse Alexa Flour 647, 1:100 (Invitrogen, #28181. Stained larvae were mounted using DAPI Fluoroshield (Sigma-Aldrich, #F6182). Images were collected on a Nikon A1 eclipse Ti confocal microscope.
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bioRxiv - Neuroscience 2022Quote: ... and 1 hour in secondary antibody (1:500 Alexa Fluor 568 donkey anti mouse antibody, Cat: A10037, Lot: 1917938)) with DAPI (1:1,000 Millipore Sigma, D9542-1MG). Tissue was washed 5 times for 5 minutes each in 1x PBS between each treatment and mounted on Fisherbrand Superfrost Plus slides ...