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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Cell Biology 2021Quote: ... The membrane was then incubated with the corresponding Alexa Fluor 647-conjugated secondary antibody or Alexa Fluor 555-conjugated anti-mouse IgG1 secondary antibody (Sigma-Aldrich, SAB4600301-50UL) at RT for 1 hour followed by 3 additional wash with TBST buffer ...
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bioRxiv - Microbiology 2020Quote: ... Cellular β-actin was used as a loading control and detected using a mouse anti-β-actin antibody (catalog no. A1987; Sigma-Aldrich). Horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibodies were used to detect the primary antibodies (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were added for 30 min to 1 h at the following dilutions: mouse anti-polyubiquitin FK1 (1:250; EMD Millipore, 04-262), guinea pig anti-p62 (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated overnight at 4°C with the following primary antibodies: mouse anti-DCP2 (Sigma, Saint Louis, MI, USA; 1:20), rabbit anti-LSm4 (Sigma ...
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bioRxiv - Physiology 2020Quote: ... The other half of the conditioned media was subjected to IgG pull-down step (2 µl for 9 ml CM) (Anti-mouse IgG monoclonal Ab; Sigma-Aldrich; Cat#M9144) which was used as a negative control for this assay ...
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bioRxiv - Neuroscience 2021Quote: ... subjected to heat induced antigen retrieval in a citrate buffer and incubated with a mouse anti-NeuN antibody (clone A60, Millipore, MAB377, 1:100). The used secondary antibodies were mouse linker antibody (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the following primary antibodies for one hour at room temperature: mouse anti-α-tubulin (DM1α) 1:500 (Millipore Sigma #T6199), rat anti-tubulin clone YL1/2 1:1500 (Millipore Sigma #MAB1864) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-FLAG rabbit polyclonal and mouse monoclonal (Cat# F7425, RRID: AB_439687 and Cat# F1804, RRID: AB_262044, respectively, Sigma-Aldrich, St. Louis, MO, USA), anti-GFP rabbit polyclonal (Cat# A-11122 ...
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bioRxiv - Genetics 2022Quote: ... in PBS and incubated with rabbit polyclonal vault antibodies (N2-B15, provided by Leonard Rome, dilution 1:1000) and mouse anti-NeuN antibodies (MAB377, Millipore, dilution 1:1000) in 0.1%NDS/PBS at 4 °C overnight under agitation ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Sections of the brainstem that contained CTB labeled neurons or mCherry neurons were incubated in a solution containing a mouse anti-TH monoclonal antibody (1:10,000; T-2928, Sigma-Aldrich, Oakville, ON, Canada) for 2 days ...
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bioRxiv - Biochemistry 2022Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, Cat No A2179) in PBST ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with TBST three times for 5 minutes and incubated with a 1:25,000 dilution of horseradish peroxidase-conjugated anti-mouse (Sigma, Cat no. # A9044-2ML6) or anti-rabbit (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum containing 0,3% Triton in PBS for 1 h at RT followed by incubation for two overnights at 4°C with mouse anti-acetylated α-tubulin (1:500, Sigma-Aldrich, T7451) and rabbit anti-βIII-tubulin (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.3% Triton-X 100 in PBS for 1 hr before a 48-hr incubation at 4°C in 1:1,000 mouse anti-TH (Millipore; St. Louis, MO, USA) and 1:3,000 rabbit anti-cFos (Millipore) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections incubated for 24 hours at 4°C in a primary antibody solution containing mouse anti-NeuN (catalogue no. MAB377; Millipore, Billerica, MA, USA) diluted 1:250 in 0.3% Triton-X and 3% normal donkey serum (NDS ...
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bioRxiv - Immunology 2021Quote: ... Samples were then incubated in secondary antibody conjugates Atto 647N (1 μg/mL each of anti-mouse; Sigma catalog no. 50185-1ML-F) for 60min ...
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bioRxiv - Genetics 2022Quote: ... For normalization of signals, we used actin or Pgk1, which was detected by using mouse anti-actin monoclonal antibody (1:5000, (Millipore Cat# MAB1501, RRID:AB_2223041)) or mouse anti-Pgk1 antibody (1:10000 ...
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bioRxiv - Neuroscience 2019Quote: Cultured human cortical neurons of 100-120 days post-differentiation were immunostained as above with primary antibodies against MAP2 (Mouse-anti-MAP2, Sigma, 1:200 dilution), VGLUT1 (Mouse-anti-VGLUT ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed three times in 1x TBST and then incubated for 1 hour at 22°C in anti-mouse IgG HRP conjugate secondary antibody (Sigma, 2834819; 1:1000). Membranes were washed three times in 1x TBST before adding enhanced chemiluminescence detection reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... polyclonal rabbit anti-mouse IgG–HRP (A9044, 1:5,000), polyclonal rabbit anti-goat IgG-HRP (A5420, 1:5,000) were from Sigma Aldrich (St. Louis, MO, USA).
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in a triton-TBS containing 2% NGS and a mouse anti-oxytocin polyclonal antiserum (MAB5296, Millipore, Burlington, MA; 1:300) for 72 hrs at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... rabbit polyclonal anti-p[Thr202]ERK44/p[Thr204]ERK42 (1:10,000; product # 9101, Cell Signalling Technology) or mouse monoclonal anti-α-tubulin (1:50,000; product # T5168, Sigma-Aldrich, Oakville, Ontario, Canada). Membranes were then rinsed 4 times for 5 minutes with 0.1 % Tween 20/tris-buffered saline at room temperature ...
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bioRxiv - Neuroscience 2021Quote: Brains of male and female mice expressing EYFP in MC3R neurons were collected and processed as describe above and neurons expressing TH were labeled using standard immunohistochemical (IHC) techniques (as described above for c-fos) using mouse anti-TH antibodies (Catalog # MAB318, Millipore, Inc., Billerica, MA) diluted 1:1500 followed by Alexa Fluor 594 conjugated goat anti-mouse antibodies (Catalog # A-11032 ...
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bioRxiv - Cell Biology 2021Quote: ... Permeabilized platelets were incubated with 1:500 dilution of mouse monoclonal anti-α-Tubulin IgG (Clone DM1A, Cat.No. T9026, Sigma Aldrich GmbH, Germany) primary antibody diluted in 0.5% saponin with 0.2% BSA in PBS for 16 hours at 4°C followed by three washing steps in PBS for 5 min each ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was carried out at 4°C overnight with the following antibodies: mouse anti-Calbindin-D-28K (Sigma, C9848, 1:1000), rabbit anti-vGlut2 (Synaptic Systems ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at room temperature and incubated overnight at 4 °C with mouse anti-FLAG monoclonal antibody (Catalogue no. F3165, Millipore Sigma, MO, USA) diluted 1:1000 in Milk/PBST ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated for 1 h at room temperature with sheep anti-mouse horseradish peroxidase (HRP)-conjugated antibody (Catalogue no. GENA931, Millipore Sigma, MO, USA) diluted 1:10000 in Milk/PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4 °C with blocking solution containing the primary antibodies rabbit anti-allograft inflammatory factor 1 (Iba1, dilution 1:200; #019-19741, Fujifilm Wako, Japan) and mouse anti-β-actin (dilution 1:400, Sigma-Aldrich #A3854). Cells were then incubated for 1 hour with the secondary antibodies AF488-conjugated anti-rabbit IgG (dilution 1:500 ...
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bioRxiv - Cell Biology 2024Quote: The sample was incubated overnight (12Lhours) at 4 °C with a primary mouse anti alpha- and beta-tubulin IgG antibodies (Sigma-Aldrich (REF: T5168) and Sigma-Aldrich (REF ...
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bioRxiv - Neuroscience 2023Quote: The following commercially available antibodies were used for the immunostaining analyses: mouse anti-HA (clone HA-7, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-PSD95 (clone K28/43 ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were placed back in the slide holder and 150 µL of the primary antibody (anti-NeuN Mouse, EMD Millipore Corp., #MAB377) diluted in RNAscope® Co-Detection Antibody Diluent (Advanced Cell Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of mouse anti-FLAG antibody (Sigma-Aldrich; F1804-1MG) and incubated at RT for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... Sections were incubated in a primary antibody cocktail solution directed against calbindin (CALB, Mouse Monoclonal Anti-Calbindin-D-28K (Cat #C9848, Sigma, St. Louis, MO)) with a 1:10,000 dilution in 2% normal horse serum ...
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bioRxiv - Cancer Biology 2023Quote: ... for two hours and probed overnight in TTBS/1% skim milk with a 1:3000 dilution of mouse anti-α-Tubulin (Sigma Aldrich #T6199), or a 1:500 dilution of mouse anti-Erα antibody (SCBT sc-56833) ...
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bioRxiv - Bioengineering 2023Quote: ... Dy-Light Lectin dye at 647 (1:1000) and an anti-smooth muscle actin antibody (α-SMA, 1:500, Millipore, Cy3, mouse monoclonal) were then incubated with tissue for approximately 24 hours ...
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bioRxiv - Systems Biology 2024Quote: Cells were analysed for protein expression by SDS polyacrylamide gel electrophoresis and Western blot analysis using the following antibodies: mouse monoclonal anti α-tubulin (#05-829, Merck Millipore, Burlington, US), rabbit α-p-AKTSer473 (#9271) ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradient was fractionated and the peak γ-TuRC fraction was determined by Western blot using mouse monoclonal anti-γ-tubulin (T6557, Sigma Aldrich) and negative stain EM ...
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bioRxiv - Developmental Biology 2024Quote: ... Puromycin incorporated into the proteins were detected in a Western blot using a mouse-origin anti-puromycin monoclonal antibody (12D10, Sigma MABE343, lot # 3484967) and a goat-anti-mouse HRP-conjugated secondary antibody (Abcam ab6789 ...
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bioRxiv - Biophysics 2024Quote: ... 1%DMSO in PBS) and incubated overnight with mouse monoclonal ANTI-FLAG® M2 antibody (Cat. F1804, Sigma-Aldrich, Inc., St Louis, MO) at a concentration of 1:800 in K-block ...