Labshake search
Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The blot was blocked in 5% milk with TBS-0.1%Tween for 2 hours at room temperature on a rocker and then incubated with indicated primary antibodies overnight at 4°C (1:1000 mouse anti-degron (Sigma-Aldrich) or 1:5000 mouse anti-tubulin (ThermoFisher)) ...
-
bioRxiv - Cell Biology 2023Quote: ... Tubulin labeling was carried out with a mouse monoclonal anti-acetylated tubulin antibody (T6793-6-11B-1, Sigma-Aldrich, 1:250) and followed by either a goat anti-mouse IgG Cy3 (115-165-166 ...
-
bioRxiv - Genetics 2023Quote: ... Primary antibody was anti-acetylated α-tubulin mouse monoclonal clone 6-11B-1 (1:500 dilution, Sigma, T7451-200, lot #077M4751V). Secondary antibody was Alexa Fluor 488 donkey anti-mouse IgG (1:1,000 dilution ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 % SDS and 100 mM β- mercaptoethanol] at 60 °C for 45 min and reprobed with mouse monoclonal anti-β-actin antibody (0.4 μg/ml; Sigma Aldrich) overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL ...
-
bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were incubated overnight at 4 °C in a humidified chamber: mouse anti-glial fibrillary acidic protein (GFAP) (1:500; Sigma-Aldrich), rabbit anti- laminin 1α (1:200 ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (Cat#MAB-318, Millipore Sigma) at 1:1000 dilution ...
-
bioRxiv - Biochemistry 2022Quote: ... The produced PSGL-1/mIgG2b were purified form the cell culture supernatants using goat anti-mouse IgG agarose beads (Sigma–Aldrich). Each enzyme (2 µM ...
-
bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-HA (rabbit monoclonal from Cell Signaling Technology Inc., cat. no. 3724S; and mouse monoclonal from Sigma-Aldrich, cat. no. H3663), anti-myc (mouse monoclonal clone 9E 10 from the University of Iowa Hybridoma Bank) ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed by incubating 1 mg of the whole cell lysates (input) with M2 mouse anti-FLAG (F3165, Sigma Aldrich) and 50 µL of microMACS Protein G magnetic beads (Miltenyi ...
-
bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence (IF): mouse anti-KHC (clone H2; MAB1614; recognizes primarily KIF5C34) (1:100, EMD Millipore); goat anti-KLC1 (V-17 ...
-
bioRxiv - Microbiology 2022Quote: ... the blots were incubated at room temperature on a rocker for 1 hr with secondary anti-mouse IgG-HRP (Sigma, A4416) or anti-rat IgG-HRP (Thermo ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
-
bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... Goat anti-IgG rabbit alkaline phosphatase conjugated secondary antibody (A9919) and goat anti-IgG mouse alkaline phosphatase conjugated secondary antibody (A2429) were purchased from Sigma Aldrich.
-
bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labelled secondary antibody (1:10) (Sigma-Aldrich) and 10 μl of anti-rabbit 5 nm gold-labelled secondary antibody (1:10 ...
-
bioRxiv - Microbiology 2023Quote: ... wells were incubated with anti-mouse IgG peroxidase-conjugated (1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) at RT for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... An additional washing step was followed by a 1 h incubation at RT with the primary mouse anti-c-myc antibody (500 µl, 1:200, Sigma Aldrich). Further ...
-
bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labeled secondary antibody (1:10) (Sigma-Aldrich). Further ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
-
bioRxiv - Neuroscience 2023Quote: ... every 4th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with mouse anti-FLAG monoclonal antibody (1:500; MAB3118, Sigma-Aldrich) or mouse anti-HA monoclonal antibody (1:500 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
-
bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hour at room temperature with a horseradish peroxidase conjugated anti-mouse secondary antibody (Cat# NA931-1ML, Millipore Sigma) diluted 1:1000 in 3% milk ...
-
bioRxiv - Microbiology 2023Quote: ... anti-p24 mouse monoclonal antibody (1: 500; MAB7360, R&D, Minneapolis, MN, USA) and anti-ZCCHC3 mouse monoclonal antibody (1: 500; SAB1408147, Sigma-Aldrich). The following secondary antibodies were used ...
-
bioRxiv - Biophysics 2023Quote: ... we weakened N-cadherin-mediated cell-cell adhesion of SMCs [49] by using the medium with the addition of mouse monoclonal anti-N-cadherin antibody (10 µg/mL, C3865, Sigma-Aldrich). The SMCs were cultured for 48 h on trough-shaped concavity arrays ...
-
bioRxiv - Immunology 2023Quote: ... Overnight incubation with mouse anti-HLA class I-ABC and 4h incubation with goat anti-Mouse Alexa Fluor 488 (Supplementary Table S5) and DAPI (1 mg/mL, Sigma-Aldrich) were performed in 10% FCS and 0.2% Triton X-100 in PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... Tubulin was used as the loading control for normalization and probed with Mouse anti-Tubulin antibody (SIGMA, 1 in 3000 dilutions). All the immunoblots of GFP variants were developed ...
-
bioRxiv - Neuroscience 2023Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich (A2179)) in PBST ...
-
bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
-
bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
-
bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
-
bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
-
bioRxiv - Genetics 2023Quote: ... The biotin molecules incorporated into the DNA were visualized by indirect immunofluorescent labeling using 1:60 diluted mouse anti-biotin as a primary antibody (Sigma-Aldrich) applied in 1% BSA/1×PBS/0.1% Triton X-100 for 45 min at RT in a humid chamber ...
-
bioRxiv - Cell Biology 2023Quote: ... The fixed animals were washed sequentially in Borate-Triton buffer (with added βME) and then incubated at 4°C overnight in mouse anti-acetylated tubulin primary antibody (Ab) (Sigma T6793), at a dilution of 1:100 in 0.5% PBST ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
-
bioRxiv - Immunology 2022Quote: ... Ultrathin sections of 50 nm were blocked with 5% FBS/5% NGS for 30 minutes and subsequently incubated with mouse anti-FLAG (Sigma F3165) for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... The cells were then incubated overnight at 4°C in primary antibody solutions with anti-α-tubulin (mouse monoclonal, 1:1,000, T9026, Sigma-Aldrich) or anti-TOMM20 (Rabbit polyclonal ...
-
bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568/AttoN647-conjugated goat anti-mouse or anti-rabbit (1:100–1:400, Thermo Fischer Scientific) and Alexa 488/568-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
-
bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
-
bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... were dissected and immunostained as described previously.77 Dissected and fixed tissue was incubated overnight at 4°C with anti-Flag (mouse, 1:200; Sigma F1804) or anti-HA (mouse ...
-
bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... MH27 mouse monoclonal antibody (from supernatent of cells provided by R. Waterston) was used with rhodamine-conjugated goat anti-mouse secondary antibody (Sigma-Aldrich) to stain fixed animals of different stages.
-
bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
-
bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...