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Citations for Millipore Sigma :
7501 - 7550 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... brains were first cut into 50 um slices and store at 4C in .1% sodium azide PBS before undergoing NeuN staining procedures using Alexa Fluor 488 Conjugate ABN78A4 Anti-NeuN (rabbit) antibody (Sigma-Aldrich). Slices were washed 3 times for 10 minutes with 1x PBS and pre-incubated in 10% Horse Serum and 0.3% Triton-X-100-PBS with 1% BSA for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, 1:10.000 dilution) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Smooth muscle cells were identified by immunostaining with fluorescein isothiocyanate (FITC)-conjugated primary antibody against α-smooth muscle actin (1:100; clone 1A4, Sigma), followed by anti-FITC biotin-conjugated secondary antibody (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... After incubation of primary antibodies at 4°C overnight and secondary antibodies for 1 h at RT (Alexa Flour 488 and 594) cells were embedded in Mowiol (Sigma-Aldrich) and subjected to imaging with the software Axiovision 4.8 at the microscope Axio Observer Z.1.
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bioRxiv - Neuroscience 2022Quote: ... was used as a substrate for in situ hybridization and immunolabeling was carried out with primary antibodies anti-GABA (1:500, Sigma–Aldrich), anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL time points were taken and quenched with 2 mM EDTA then brought to 20 μL final volume for detection using 1:500 dilution anti-H3K4me3 antibody (05-745R, EMD Millipore), 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082) ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was permeabilized/blocked in a solution containing 1% Triton-X and 10% NGS in PBS for 30 min and then incubated with an anti-HNE-Michael adducts rabbit polyclonal antibody (1:150 dilution, EMD Millipore) in a 10% NGS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The following antibodies were used in the experiment: phospho(p)-IR tyr972 (1/800, 155 kDa; 07-838, Sigma-Aldrich, USA), p-IRS1 r307 (1/800 ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 μg of lysates were incubated with 1 μg of mouse α-Flag M2 antibody (Sigma-Aldrich #1804; St. Louis, MO) in a final volume of 1 mL of immunoprecipitation buffer overnight at 4°C with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody is diluted in blocking buffer and slides are incubated in primary antibody overnight at 4°C (ChAT, Millipore, AB144P, goat, 1:200; vGlut1, EMD Millipore, AB5905 ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS and incubated with secondary antibodies at 1:500 dilution (goat anti-rabbit Alexa 594, Sigma Aldrich,) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... iBECs were cultured in Transwell inserts with 3.0 µm pores and exposed to FITC-conjugated 150 kDa dextran (0.5 mg/ml) or anti-TDP-43 antibody (1 µM, Sigma, Cat#T1705) and 10 µl of phospholipid-shelled microbubbles with octafluoropropane gas core prepared in-house as described in (40) ...
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bioRxiv - Neuroscience 2024Quote: ... for one hour before overnight incubation at 4℃ with one of the following primary antibodies: anti-NG2 (1:200; Millipore AB5320), anti-Laminin (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed five times with five-minute incubations in PBST and then incubated overnight in primary antibody solution containing anti-acetylated alpha-tubulin (1:500; Sigma, T6793) in PBST plus 20% NGS ...
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bioRxiv - Immunology 2024Quote: ... Viral nucleoprotein (NP) was detected by ELISA as described using a 1/1,000 dilution of the anti-NP antibody (Millipore cat. #MAB8257) and a 1/2,000 dilution of the horseradish peroxidase-labeled goat anti-mouse IgG (SeraCare cat ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... Overnight primary antibody incubation was performed on a rocker in blocking solution containing the Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was blocked in 5% milk with TBS-0.1%Tween for 2 hours at room temperature on a rocker and then incubated with indicated primary antibodies overnight at 4°C (1:1000 mouse anti-degron (Sigma-Aldrich) or 1:5000 mouse anti-tubulin (ThermoFisher)) ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence (IF): mouse anti-KHC (clone H2; MAB1614; recognizes primarily KIF5C34) (1:100, EMD Millipore); goat anti-KLC1 (V-17 ...
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bioRxiv - Cell Biology 2023Quote: ... each coverslip was washed 3 times in PBS then placed on top of 50 µL drops of the secondary antibody mixture of goat anti-rat TRITC (1:100; Millipore Sigma) and goat anti-mouse FITC (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... BSA in Tris buffered saline and Tween 20 (TBS-T) buffer for 1 h and incubated overnight with the following primary antibodies: Anti-LINE1 ORF1p (MABC1152) (EMD Millipore), Recombinant Anti-LINE1 ORF1p antibody [EPR21844-108] (Abcam) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were incubated with primary antibodies overnight in a humidified chamber at 4°C with α-actinin (1:1,000, Sigma, A7811). Slides were incubated with secondary antibody (Alexa Fluor Plus 488 ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and incubated overnight at 4°C with primary antibodies to label cells containing tyrosine hydroxylase (anti-tyrosine hydroxylase monoclonal, 1:1000 Millipore, MAB318) and mCherry (anti-dsRed polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:1,000 dilution, Millipore-Sigma, Cat# MAB318, RRID: AB_2201528) and guinea pig anti GFP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Microbiology 2023Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysates were then immunoprecipitated overnight at 4°C with TrkA antibody (1:100, 06-574, Sigma-Aldrich, St. Louis, MO, USA) or p75NTR antibody (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labelled secondary antibody (1:10) (Sigma-Aldrich) and 10 μl of anti-rabbit 5 nm gold-labelled secondary antibody (1:10 ...