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Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4°C with primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500; Millipore Cat# AB144P, RRID:AB_2079751), mouse anti-parvalbumin (PV ...
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bioRxiv - Neuroscience 2019Quote: ... before being incubated (4 h at room temperature followed by overnight at 8 °C) in a mixture containing the primary antibodies made up in a block buffer comprising 1 % bovine serum albumin (Sigma-Aldrich), 0.1 % porcine gelatine (BDH) ...
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bioRxiv - Biochemistry 2021Quote: ... Bound proteins were eluted by addition of NuPAGE LDS gel loading buffer and proteins in the supernatant and pellet were analysed by gel electrophoresis followed by western blotting using the following antibodies: anti-HOIP (#SAB2102031) and anti-HOIL-1 (#HPA024185) were from Sigma-Aldrich; anti-MBP (#E8032S ...
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bioRxiv - Neuroscience 2020Quote: ... equilibrated in TN buffer (150 mM NaCl and 100 mM Tris pH= 7.5 in water) and incubated for 30 min in 1:2000 HRP-coupled anti-DIG antibody (Sigma-Aldrich, 11207733910). Following the incubation ...
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bioRxiv - Microbiology 2021Quote: ... DivK protein separated based on its phosphorylation state was transferred to a PVDF membrane and detected using a chemiluminescent substrate (SuperSignal West PICO PLUS, Thermo Pierce) after incubation with mouse anti-Flag (1:10000) primary antibody (Sigma, F1804) and a polyclonal goat anti-mouse HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... After three washes in PBS sections were incubated with secondary antibodies (anti-Rabbit IgG CF™ 568, 1:1000, Sigma Aldrich, SAB4600084 ...
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bioRxiv - Bioengineering 2020Quote: ... the primary antibody that was used was mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend (EMD Millipore, MAB8251, 1:250). Images were taken at 10x magnification using a Zeiss Axio Observer and Axiocam 503 mono camera (Zeiss).
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with a goat anti-choline acetyltransferase antibody (ChAT; 1:1000; Millipore Cat# AB144P, RRID:AB_2079751) in Tris-Triton buffer containing 2% donkey serum and 0.2% Triton X100 ...
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bioRxiv - Neuroscience 2021Quote: ... affinity-purified rabbit anti-panx1b antibody, code#ZOI-B2, Davids Biotechnologie GmbH; 1:100, rabbit anti-GFAP, code AB5804, Sigma-Aldrich) overnight at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... 1:500 dilution of Rat polyclonal anti-GnRH antibody (Skrapits et al., 2015) and 1:250 dilution Guinea Pig polycloncal anti-NeuN (EMD Millipore). After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then rinsed with PBS and allowed to incubate in primary antibodies for wisteria floribunda agglutinin (PNNs; 1:1,000; Sigma-Aldrich L1516) and parvalbumin (PV+ ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... secondary antibodies conjugated with Abberior® STAR RED/580 dyes were used at 1:200 dilution (#41699 and #52405, Millipore Sigma). After secondary antibody incubation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Remaining cell lysate was incubated with pre-washed (three times in 1 ml lysis buffer) 20 μl of antibody-conjugated beads/resin (FLAG: EZview Red ANTI-FLAG M2 Affinity Gel, Sigma-Aldrich; HA ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... DNA-primary antibody conjugates were washed with 1× PBS four times and concentrated using 50 KDa Amicon Ultra Centrifugal Filters (Millipore, UFC505096). The concentration of conjugated oligo DNA and antibody with BCA Protein Assay Kit (Thermo Scientific 23225 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with 5% skimmed milk for 1 hour at room temperature and then with primary antibodies diluted in 2% bovine serum albumin (Sigma-Aldrich) in tris-buffered saline 0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight with primary antibody against tyrosine hydroxylase diluted in 2% NDS in PBS/TX (1:1000 - rabbit anti-TH, Millipore AB152). The sections were rinsed with PBS (3×10min ...
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bioRxiv - Neuroscience 2020Quote: Cell type specific imaging using fluorescent markers: dopaminergic neurons were specifically labelled using the anti-Tyrosine Hydroxylase (TH) antibody (1:250, rabbit polyclonal, AB152, Sigma Aldrich). This was done on 50 μm thick coronal sections in the midbrain area specifically for the neurons in the ventral tegmental area (VTA ...
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bioRxiv - Neuroscience 2020Quote: ... Position in the retina was determined by staining with a primary antibody sensitive to S-opsin (rabbit-anti-s-opsin, 1:500, Millipore ABN1660) (Sondereker et al. ...
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bioRxiv - Microbiology 2021Quote: ... then washed and probed with a 1:2,000 dilution of an α-rabbit alkaline phosphatase conjugated antibody (Sigma Aldrich, cat # A3687) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for two days at 4°C in solution containing the primary antibody for choline acetyltransferase (Goat-ChAT, 1:100 dilution, Millipore AB144P). When enhancement of GCaMP ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... in Triton PBS containing 1% v/v NDS and a mixture of between two and four of the following primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500 dilution; AB144P, Millipore, RRID:AB_2079751); goat anti-forkhead box protein P2 (FoxP2 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cell Biology 2021Quote: ... and immunoblotting was performed using the following antibodies: anti-K63 ubiquitin (1:4,000; EMD Millipore, cat. no. 05-1308, clone apu3), anti-actin (1:6,000 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... TA muscles were carefully removed and then frozen in liquid nitrogen and stored (−80 °C) for immunoblotting analysis using a mouse IgG2a monoclonal anti-puromycin antibody (clone 12D10, 1:2500; Millipore # MABE343).
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was removed and replaced with 500 μl of a solution containing a 1:500 dilution of anti-tyrosine hydroxylase antibody (Millipore Sigma). Slices were incubated overnight with side to side rotation at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 4 °C with the respective species-specific fluorescently-labelled antibodies and DAPI (1 μg/mL, Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for one hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with the primary antibodies (12h at 4 °C): anti-ChAT (1:500, goat, Sigma-Aldrich, AB144P). Then the sections were washed in PBS five times at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed by an overnight incubation at 4°C with rabbit anti-HA antibody (1:1000, Cat no. H6908, Sigma-Aldrich, USA). For c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...