Labshake search
Citations for Millipore Sigma :
7601 - 7650 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... 2003) and a goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (1:4000, Chemicon®, Sigma-Aldrich, www.sigmaaldrich.com, #AP307P). Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... Probed membrane was washed with TBS-T thrice before incubating with alkaline phosphatase-conjugated anti-mouse secondary antibody (1:10000 dilution, Sigma, USA) or goat anti-rabbit IgG (H+L ...
-
bioRxiv - Neuroscience 2023Quote: ... we blocked membranes with 5% milk in PBS for 1 hour at room temperature and probed overnight with rocking at 4°C with the following primary antibodies: mouse anti-FLAG clone M2 (1/1000 dilution, Sigma # F1804), mouse anti-HA clone 16B12 (1/3000 dilution for HEK-293 cell lysates ...
-
bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C with the primary antibodies (diluted in 1% bovine serum albumin (BSA, cat. no.: A3912-50G, Sigma-Aldrich) in tris-buffered saline (TBS ...
-
bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with 5% BSA at room temperature for 1 hour and incubated with anti-FLAG M2-peroxidase antibody (Sigma-Aldrich) at room temperature for another hour ...
-
bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
-
bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
-
bioRxiv - Developmental Biology 2024Quote: ... and transferred to a 24-well plate and incubated overnight at 4 °C with shaking in 400 µL of primary antibody solution: DAPI 1:1000 (D9542-1g, Sigma-Aldrich), Phalloidin 1:100 (#A12379 ...
-
bioRxiv - Biophysics 2024Quote: ... Immunostaining was performed as previously described [84] using the following antibodies: Mouse anti-Rac1 (Millipore Cat#05-389-25UG, 1:200), Rabbit anti-RhoA (Proteintech Cat#10749-1-AP ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
-
bioRxiv - Neuroscience 2024Quote: ... the selected sections were incubated in a 1:300 dilution of goat polyclonal antibody against ChAT (Millipore Cat# AB144P, RRID: AB_2079751), 2% NDS and 0.5% Triton-X-100 in 0.1 M PBS overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... The axons were labeled with a monoclonal antibody against neuron-specific class III p-tubulin (clone Tuj-1 Babco) and the nuclei with DAPI (Sigma Aldrich). Pictures of explant were taken using a Leica fluorescent microscope and a Photometrics Coolsnap fx monochrome CCD camera (Roper Scientific ...
-
bioRxiv - Genomics 2024Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... dorsal and ventral transverse slices were incubated with the following primary and secondary antibody combinations using the protocol described: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies ...
-
bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Tissue sections on Visium Spatial Gene Expression Slide were fixed with methanol at −20 °C for 30 min and labelled with an anti-αSMA antibody (Cy3 conjugated, 1:2000, Sigma C6198) for 30 min at room temperature followed by DAPI nuclear counter stain ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were exposed to antibodies against NeuN (neuronal marker; 1:1,000, clone A60, Merck Millipore, cat#MAB377, Temecula, CA, USA), MAP2 (neuronal marker ...
-
bioRxiv - Immunology 2022Quote: ... isolated decidual tissues were cut into small pieces then digested in 1.25 U/mL Dispase II (Sigma, D4693)/0.4 mg/mL collagenase V (Sigma ...
-
bioRxiv - Biochemistry 2019Quote: ... Incubation was for 16 h at 37 °C with Copper(II) chloride (Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 5 µM ...
-
bioRxiv - Cell Biology 2020Quote: ... the remaining mesenchymal fraction was incubated with 6 mg/ml Dispase II/0.05% trypsin solution (Sigma-Aldrich, 04942078001) supplemented with 1 U/ml DNaseI (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... Image of each cell was acquired while they were run on the ImageStreamX Mark II (Amnis-EMD Millipore) at 5 × 107 cells per mL in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... 16HBE cells were grown on 8-well glass slides (Nunc Lab-Tek II chamber slide system; Sigma-Aldrich) with each well seeded with 450 µl of cell suspension at 1.2 x 105 cells/ml and grown for 24 hours ...
-
bioRxiv - Cancer Biology 2020Quote: ... tumors were harvested and enzymatically disassociated using the Animal free Collagenase/Dispase Blend II reagent (Millipore, Burlington, MA). Cells were maintained in culture with RPMI-1640 media (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cells were washed with buffer A from the Duolink II proximity ligation assay kit (Olink Bioscience, Sigma), followed by the addition of Duolink II PLA probe anti-mouse Minus and Duolink II PLA probe anti-rabbit Plus ...
-
bioRxiv - Neuroscience 2022Quote: Vero E6 cells were maintained in EMEM (ATCC) media with 10% Serum Plus II Medium Supplement (Sigma-Aldrich). SARS-CoV-2 virus were obtained from the CDC following isolation from a patient in Washington State (WA-1 strain - BEI #NR-52281) ...
-
bioRxiv - Bioengineering 2019Quote: ... The reservoirs were filled with EHT medium (Stemline II Hematopoietic Stem Cell Expansion Medium S0192-500 Sigma-Aldrich) supplemented with 50 ng mL−1 SCF ...
-
bioRxiv - Biochemistry 2019Quote: ... Collagen II was a pepsin-digested guanidine-HCl-extract from a bovine joint cartilage purchased from Sigma-Aldrich/Merck ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell viability was assessed after 72h incubation using a home-made Alamar Blue solution (75mg resazurin, 12.5mg methylene blue, 164.5mg potassium hexacyanoferrate III and 211mg potassium hexacyanoferrate II trihydrate, 500ml water; Sigma) as previously described [12] ...
-
bioRxiv - Neuroscience 2022Quote: ... culture medium I was replaced with culture medium II (DMEM supplemented with 24 μg/ml insulin (Sigma Aldrich), 100 μg/ml transferrin (EMD Millipore) ...
-
bioRxiv - Physiology 2024Quote: ... The washed epithelium was digested in 10 mL of digestion buffer (HBSS with 0.3 mg/mL dispase II [Sigma] and 0.2 mg/mL DNaseI [Sigma]) at 37°C for 8 minutes ...
-
bioRxiv - Genomics 2024Quote: ... SCD-complete was supplemented with 750 µM Ferrozine +/- 300 µM Ammonium iron(II) sulfate (Sigma 160601 and 215406). Cells were grown at 30 °C until OD600 = ∼ 0.5 and further diluted to OD600 = 0.05 in the appropriate media ...
-
bioRxiv - Cell Biology 2023Quote: ... skin samples were digested at 4°C overnight in 5 mg/mL dispase II solution (#D4693 Sigma-Aldrich) in HBSS (#H8264 Sigma-Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... After incubation with 500 U/mL collagenase IV and 2.5 U/mL dispase II (both from Sigma Aldrich) for 60 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... The tissues were treated for 30-60 min with 2.5 U/ml of Dispase II (4942078001; Sigma Aldrich) in PBS at +4⁰C in the shaker and then 3-4 min with a pancreatin-trypsin (2.5 mg/ml pancreatin [P3292 ...
-
bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No ...
-
bioRxiv - Immunology 2023Quote: ... samples were cut into thirds and incubated overnight at 4°C in 5 mg/mL Dispase II (Sigma) in Hanks Buffered Salt Solution (HBSS) ...
-
bioRxiv - Cell Biology 2024Quote: ... instead of 0.5% AlbuMAX II and 30 μM each of oleic and palmitic acid (Sigma #O1008 and #P0500). After completion of the invasion cycle ...
-
bioRxiv - Immunology 2024Quote: ... biopsies were incubated at 37 degrees C for 30 minutes with Collagenase II (Sigma-Aldrich, 700 U/mL) and DNase I (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2024Quote: Samples for PLIM experiments were prepared by adding 500 μM dichlorotris (1,10-phenanthroline) ruthenium (II) hydrate (Sigma Aldrich) to condensate solutions (23) ...
-
bioRxiv - Bioengineering 2020Quote: ... Phosphate buffered saline powder (PBS, D5652), collagenase from Clostridium histolyticum (Type I, C0130) and Triton-X 100 (T8787) were purchased from Sigma-Aldrich (St Louis, MO, USA). Foetal bovine serum (35010CV ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... we mixed 500 mL tap water with 38 g gelatin from porcine skin (gel strength 300, Type A, G2500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 25 g honey (Gut & Günstig Bienenhonig flüssig ...
-
bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
-
bioRxiv - Molecular Biology 2019Quote: CGC were collected 4 h after OPU during oocyte denudation lasting up to 5 min with type IV-S hyaluronidase extracted from bovine testes (Sigma-Aldrich, St-Louis, MO, USA) and diluted in Sperm Preparation Medium (Origio) ...
-
bioRxiv - Microbiology 2021Quote: ... ovatus cells were grown to mid-exponential phase on either purified mucin O-linked glycans (purified in house from Sigma Type III porcine gastric mucin) or glucose as a reference as previously described (22) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The DNA samples were loaded to 0.49 ug and run on a 1.1% agarose gel (Sigma-Aldrich Type I-A, low EEO catalog no. A0169) in 1x Tris/acetic acid/EDTA (TAE ...
-
bioRxiv - Bioengineering 2023Quote: ... 5 g of powdered gelatin sourced from porcine skin (Type A, with a Bloom strength of 300, obtained from Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 50 mL of RO purified water ...
-
bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
-
bioRxiv - Developmental Biology 2023Quote: ... final concentration 20 μM in Figure3 d-e and 40μM in Figure3 g-h) or Collagenase type IV (Sigma C5138, final concentration 20 μg/ml). Control explants were cultured with BME supplemented with the drug vehicle ...
-
bioRxiv - Neuroscience 2023Quote: HUVECs transfected with RNF213 wild-type (WT) or R4810K under OGD conditions were fixed in 2% glutaraldehyde solution (15720, Sigma-Aldrich, Saint Louis, MO, USA). After fixation ...