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Citations for Millipore Sigma :
7501 - 7550 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and then intracellularly stained with Peridinin chlorophyll protein-Cy5.5-conjugated anti-mouse TNF-α (Millipore, Billerica) and phycoerythrin-conjugated anti-mouse IL-4 (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were concentrated by using the Amicon Ultra centrifugal device (NMWL: 3K) (Merck, Millipore Cat# UFC500324), and the concentration was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23225) ...
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bioRxiv - Physiology 2022Quote: ... Hyaluronic acid/hyaluronan (HA) was stained overnight using biotinylated HA binding protein (Millipore, Burlington, MA; 385911). All sections were washed and incubated with VECTASTAIN® ABC-HRP kit (Vector laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Protein reduction and alkylation of cysteine was performed with 10 mM TCEP (Millipore Sigma; Cat#: C4706) and 40 mM CAA (Millipore Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 167 mM NaCl) and pre-cleared with 75 µL of Protein A agarose (50% slurry, Sigma). Pre-cleared chromatin lysate was immunoprecipitated by a ChIP-grade rabbit polyclonal antibody against histone H3K9me3 (Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Captured proteins were eluted off FLAG beads using 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich). Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The same membrane was stripped of antibody and total protein was determined with Coomassie (Sigma, B0149) staining ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a 4 h of incubation with protein A/G agarose beads (LOT: 3460992, Millipore). The purified DNA fragments bound by ATF3 were analyzed using qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-protein complexes were retrieved from the cleared lysate on anti-Flag M2 beads (Sigma Aldrich) and eluted using 3x Flag peptide ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using Amicon Ultra centrifugal filters with 10 kDa cut-off (Millipore) and dialyzed overnight against storage buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... Separated proteins were transferred from the gel to a polyvinylidene fluoride membrane (PVDF, Immobilon-P Millipore) using a wet blot system (Mini-PROTEAN Tetra Cell ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biophysics 2022Quote: ... the protein was refolded via 10-fold dilution in either 5 mM oxidized glutathione (Millipore 3542) or 3.3 mM oxidized glutathione + 1.7 mM reduced glutathione dissolved in PBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Detection of an interaction between proteins was assessed using the Duolink II Detection Kit (Sigma-Aldrich) according to the manufacturer specifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 30µg protein was loaded on 10% SDS-PAGE and then transferred to PVDF membrane (Millipore) followed by blocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred onto nitrocellulose (chemiluminescence imaging) or to Immobilion FL PVDF membranes (Millipore, fluorescence imaging) using transfer buffer with 10% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Biochemistry 2024Quote: ... The affinity captured proteins were then digested with 4 μg of MS grade trypsin (Sigma- Aldrich) in 500 μL of digestion buffer [2 M urea ...
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bioRxiv - Biophysics 2023Quote: ... The retrieved protein was concentrated to 0.5 ml with Amicon Ultra (50 kDa cutoff, EMD Millipore) and followed by size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between NINJ1 and xCT proteins was assessed using the Duolink Proximity Ligation Assay (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: RIP assays were performed using the Magna RIPTM RNA-binding protein immunoprecipitation kit (Millipore, Massachusetts, USA). Cells in 15 cm dishes were lysed in complete RIP lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... expression of fusion proteins was induced for 24h by the addition of doxycycline (Sigma-Aldrich # D9891) to 5 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted immunoprecipitated protein was precipitated by adding TCA and Tween 20 (Sigma Aldrich, St. Louis, USA) to a final concentration of 10% and 0.5% respectively and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Forty micrograms of protein samples were denatured in reducing loading buffer containing β-mercaptoethanol (M3148, Sigma) and 0.0002% bromophenol blue at 95°C for 5 minutes and separated in pre-casted 12% or 7.5% Tris-Glycine polyacrylamide gel using the Criterion cell system (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: Immunostaining of proteins was conducted on fixed cells using 4% (wt/vol) paraformaldehyde (252549, Sigma-Aldrich) as previously described [61] ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The purified protein was concentrated to 0.6 mg/ml using an Amicon centrifugal contentrator (EMD Millipore), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were further separated on the 12% SDS-PAGE and transferred on the PVDF membrane (Millipore) using semi dry trans-blot (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Temecula, CA) and then blocked in 5% non-fat milk ...
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bioRxiv - Genomics 2024Quote: ... the system was calibrated using the Protein Standard Mix 15-600 kDa (Sigma-Aldrich, Darmstadt, Germany). Calibration was done under a flow rate of 0.5 mL min-1 with a mobile phase containing 50 mM Tris-HCl buffer (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred (300 mA, 3h) onto ethanol-activated Immobilon-P PVDF membrane (Millipore) before blocking with 3% Bovine Serum Albumin in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration for the total and cytoplasmic lysates were quantified by Bradford Reagent (B6916, Sigma Aldrich) and 10-20 μg of protein was used for western blot experiments ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Secreted JAGGED1-Fc was recovered from the culture media on Protein A agarose (Millipore, 16-125) and eluted with 100 mM glycine ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Immunology 2023Quote: ... The refolded protein product was concentrated by centrifugation in 3K MWCO Amicon tubes (EMD Millipore, #UFC900324) until a final concentration of at least 4 mg/mL was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates with equal amounts of proteins were transferred to anti-FlagM2 affinity gel (Sigma-Aldrich) and incubated for 1.5 hours at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were separated from beads with Corning Costar Spin-X filter units (#CLS8162, Sigma-Aldrich) and stored at -20°C until further processing.
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bioRxiv - Cell Biology 2022Quote: ... and immunoprecipitated proteins were eluted from Dynabeads by incubating with 200 μM FLAG peptides (Sigma-Aldrich) for 90 min ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were resolved on SDS-polyacrylamide gels and transferred onto PVDF Immobilon-FL membranes (Millipore, IPFL00010). Membranes were blocked with Intercept blocking buffer in PBS (LI-COR ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... Bound protein was eluted in 30 μl of elution buffer (0.15 mg/ml 3xFLAG peptide [Sigma, St Louis ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then dialyzed and concentrated using an Amicon Ultra-15 10 KDa cutoff (Millipore).