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Citations for Millipore Sigma :
7751 - 7800 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The eluted protein was concentrated using a 10 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The concentrated protein was further purified using size-exclusion chromatography with a Superdex 75 10/300 GL column (GE Life Sciences ...
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bioRxiv - Genomics 2019Quote: ... The dialyzed protein solution was concentrated using an Amicon Ultra-4 Centrifugal Filter Units 30K (Millipore UFC803024), and sterile glycerol was added to make a final 50% glycerol stock of the purified protein.
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bioRxiv - Physiology 2020Quote: ... Equal quantities of proteins were resolved by SDS-PAGE gels and blotted onto polyvinylidene difluoride membranes (Millipore). Then ...
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bioRxiv - Microbiology 2019Quote: ... The purified proteins were concentrated to 30-40 mg/mL using Amicon concentrators (30-kDa cutoff; Millipore), aliquoted ...
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bioRxiv - Genomics 2019Quote: ... DENV C protein purified fractions were concentrated with a Centriprep® Centrifugal Filter (Millipore, cutoff of 3kDa) and stored at −80 °C ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Total protein (30 μg) was separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Merck, Germany) using a transfer apparatus (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Microbiology 2019Quote: ... Equal amounts of total protein were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). The membrane was then blocked with horse blocking buffer (bioWORLD ...
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bioRxiv - Genetics 2020Quote: ... Resin-bound proteins were eluted in R buffer containing 300 mM NaCl and 40 mM glutathione (Sigma). The sample was supplemented with 0.2 µg/mL of HRV-3C protease (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2019Quote: Proteins separated by SDS-gel electrophoresis (7.5–15% polyacrylamide) were transferred to Immobilon-PSQ PVDF membranes (Millipore). After blocking with 5% non-fat dry milk in TBS (50 mM Tris·HCl ...
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bioRxiv - Systems Biology 2020Quote: ... One part was stained using Coomassie Blue for total protein content (Brilliant Blue G solution, Sigma Aldrich) and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved on SDS-polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Immobilon-P, Millipore). After being incubated with the primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants containing untagged protein were concentrated using Centriprep centrifugal filter devices (Millipore; molecular weight cutoff, 10 kDa) and loaded on superdex 75 column on an AKTA pure 25L protein purification system (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 centrifugal filters (3 kDa molecular weight cut-off, Millipore). Reducing agent (2 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma, P3775, 1:10,000) and anti-Flag (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 10 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... the purified protein containing fraction was concentrated (Centriprep® Centrifugal Filters Ultacel® YM - 50, Merck Millipore).
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
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bioRxiv - Biochemistry 2021Quote: ... Each well contained 0.5 mg/ml protein and 2.5X SYPRO orange dye (Sigma, St. Louis, MO, USA) in 10 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... After thawing the extraction buffer protein was precipitated with 100% trichloroacetic acid (final concentration 10%)(Sigma-Aldrich). After centrifugation at 12000 g for 5 min the pellet was washed with ice-cold 80% ethanol and ice-cold acetone by successive centrifugation-resuspension cycles ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The sample was then added to 75 μL of pre-washed Protein A sepharose beads (Sigma-Aldrich) and further incubated with rotation at 4 °C for an additional 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Protein degradation rate was measured after 3-hour treatment with 20μg/ml of cycloheximide (Sigma-Aldrich #C7698) in the complete RPMI medium ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transfer to PVDF membranes and detected using either monoclonal anti-GFP (1:1000; Sigma, 11814460001), anti-HA 12CA5 (1:1000 ...
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bioRxiv - Pathology 2021Quote: ... The proteins were cleaned using Amicon Ultra-4 Centrifugal Filter Devices (15 ml, 10 kD; Merck Millipore) to remove the elution buffer ...
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bioRxiv - Cell Biology 2021Quote: ... followed by the transfer of proteins to polyvinylidene difluoride (PVDF) membranes (EMD Millipore, catalog # IPVH00010, lot # R0HB88840).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS acrylamide gel electrophoresis and transferred to IMMOBILON-FL 26 PVDF membrane (Millipore) probed with the indicated antibodies and visualized either by chemiluminescence (according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Systems Biology 2021Quote: ... at a 1:50 protease/protein ratio overnight at 37 °C in 100 mM NH4HCO3 (Sigma-Aldrich). The next day ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... the protein was rebuffered and concentrated via centrifugal ultrafiltration using a column with 10-kDa cutoff (Millipore). The purified protein was stored in storage buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was finally visualized by a 0.1M PB solution containing 5% 3,3’-diaminobenzidine (DAB) (Sigma, Germany) and 0.02% H2O2 ...
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bioRxiv - Microbiology 2020Quote: ... Total protein synthesis was measured by immunoblot using the anti-puromycin antibody at 1:500 (EMD Millipore). Protein synthesis was quantified by densitometry using ImageJ and normalized by immunoblot using antibody specific to steady-state levels of eIF2α protein (13).
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bioRxiv - Systems Biology 2021Quote: Proteins in the urine supernatant were concentrated with 10-kDa Amicon Ultra-15 centrifugal filter units (Millipore). The final protein concentration was measured by bicinchoninic (BCA ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of protein lysate were separated by SDS-PAGE and transferred onto a nitrocellulose membrane (Millipore) at 30 V for at least 60 min (overnight for ubiquitination assays) ...
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bioRxiv - Plant Biology 2020Quote: ... The FLAG-tagged PCRK2 and PBL19 proteins were immunoprecipitated using 15 μl M2 beads (Sigma; Cat. #A2220). Biotinylation was detected with Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged NRG1C and SAG101 proteins were immunoprecipitated using 20 µl M2 beads (Sigma; Cat. #A2220) for co-immunoprecipitation and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody-bound chromatin complex was precipitated with salmon sperm DNA/Protein A agarose (EMD Millipore, Burlington, MA), then washed sequentially with PIRA (150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were blotted as previously described and detected using Anti-polyHistidine-Peroxidase monoclonal antibody (Sigma-Aldrich # A7058) and signals were revealed using the Metal Enhanced DAB Substrate Kit ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated by SDS-PAGE and probed with antibodies against zDHHC5 (1:1,000; Sigma Prestige, HPA014670), HA (1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 mg of protein was mixed with 20 μL of anti-FLAG M2 magnetic bead slurry (Sigma) in a 1 mL volume ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated with 30 μL of Anti-Flag® M2 Affinity Gel beads (Sigma-Aldrich) at 4°C for 4 h ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...