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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was based on the FASP procedure using 30K Amicon Ultra-4 filtration devices (Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... The total protein concentration in the cell lysates was quantified by a BCA assay (Sigma-Aldrich). Equal amounts of total proteins were separated with SDS-PAGE using 12,5% gels ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449), secondary antibody (Biorad #170-6515) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were concentrated using ultrafiltration devices with a molecular mass cut-off of 30 kDa (Millipore), and protein concentrations were measured using spectrophotometry ...
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted by mechanical homogenization and subsequent membrane lysis using ice-cold RIPA buffer (Sigma) with freshly added proteinase and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Equal amount of protein was resolved on 12% SDS-PAGE and transferred onto PVDF membranes (Millipore) by semi-dry immunoblotting method (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein sample (400 μg) was reduced by adding 2 μl of Tris(2-carboxyethyl)phosphine (Sigma) and incubating samples at 60 °C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 6 ml buffer B containing 15 mM d-desthiobiotin (Sigma Aldrich). Purification of GyrA-N-Strep and GyrB-C-Strep were conducted in the same way using an adjusted buffer Bgyr for cell disruption and purification (buffer Bgyr ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content in each well was evaluated by the SRB assay (Sulphorhodamine B, SIGMA, cat. S1402) as described elsewhere98.
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bioRxiv - Cell Biology 2021Quote: ... Sample proteins were separated by SDS-PAGE electrophoresis and transferred to low-fluorescence PVDF membrane (Millipore). Membranes were blocked for 1 hr in 3% milk in TBST prior to incubation with the indicated primary antibodies in 3% BSA overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The enriched cortical neuron population was immediately placed in 50μl RIPA protein extraction buffer (EMD Millipore) with HALT Protease/Phosphatase inhibitors (ThermoFisherScientific ...
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bioRxiv - Genetics 2019Quote: The immunoprecipitated proteins bound to the beads were digested overnight in 400 ng trypsin (Sigma, V511A) in 25 mM ammonium bicarbonate (Sigma ...
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bioRxiv - Genomics 2021Quote: Protein samples were transferred onto a immobilon-PSQ 0.2 μM pore size PVDF membrane (Merk Millipore). The PVDF membrane was blocked by 3% BSA in 0.05% PBST (0.05% Tween-20 in 1 x PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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bioRxiv - Molecular Biology 2021Quote: ... proteins extracted from cells were incubated overnight at 4°C with FLAG-M2 beads (Sigma, A2220) or pre- conjugated A/G agarose beads (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were recovered and protein concentrations determined by Lowry assay using bovine serum albumin (Sigma-Aldrich) as a standard ...
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bioRxiv - Cancer Biology 2021Quote: ... Fractions containing the defensin protein were concentrated using a Minitan II ultrafiltration system (Millipore, Bedford, MA) with a 3-kD cutoff membrane and dialyzed against 10 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein lysates were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). Quantification of the intensity of protein bands by normalizing to GAPDH was performed by ImageJ software.
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bioRxiv - Cell Biology 2022Quote: Proteins were resolved on a 4-12% SDS-PAGE gel and transferred onto PVDF membranes (Millipore) using Criterion™ Blotter (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were alkylated by addition of 55 mM iodoacetamide (Sigma Chem. Corp. St. Louis, MO) and incubation for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation was carried out with β-catenin monoclonal antibody and Protein G magnetic beads (Sigma-Aldrich). For PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were reduced with 5 mM 5-Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma-Aldrich) for 2 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... we performed a RIP assay using Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). HDFa were lysed in RIP lysis buffer ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030) and passed through two subsequent gel filtration steps using a ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was then concentrated using an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). Protein concentration and label efficiency were calculated by measuring appropriate absorbances using a Nanodrop One spectrophotometer ...
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bioRxiv - Biochemistry 2022Quote: Proteins were separated by SDS-PAGE or Nu-PAGE and transferred to PVDF membranes (Millipore; IPVH00010). After blocking with 5% skim milk ...
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bioRxiv - Bioengineering 2022Quote: ... 30 kDa cut-off Amicon-Ultra-15 protein concentrators were bought from EMD Millipore (Billerica, USA). The culture media and cellobiose were purchased from Sigma-Aldrich (St ...
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bioRxiv - Bioengineering 2022Quote: ... fibrinogen stock (4 mg of clottable protein/mL of DMEM, bovine plasma, Type I-S, Sigma) was filter-sterilized and mixed with myoblasts suspension ...
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bioRxiv - Biochemistry 2022Quote: ... Protein degradation via the 26S proteasome was blocked by adding 10 µM MG132 (Sigma-Aldrich, C2211) 3 h before the addition of cycloheximide ...
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bioRxiv - Cell Biology 2022Quote: ... followed by an overnight incubation with Pure-Proteome Protein A/G mix magnetic beads (Millipore Sigma). Samples were washed as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... staining of SDS-PAGE-separated purified proteins and Bovine Serum Albumin (BSA) standards (Sigma-Aldrich; A2153) or by using a Nanodrop spectrophotometer (ND1000).
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bioRxiv - Cell Biology 2022Quote: ... Twenty micrograms of protein were separated on 10% SDS gels and then transferred to PVDF (Millipore) membranes ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 140μg of mitochondrial protein was incubated with 1:100 of 1M dithiothreitol (Sigma-Aldrich, SKU 43815), followed by 1:50 dark incubation in 1M iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich, #7345) as indicated rotating at 4 °C for 2 hrs ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.45 μm PVDF membrane (#IPVH85R; Sigma-Aldrich/Merck, Zwijndrecht, The Netherlands) using an electroblotting device (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Both the proteins were then desalted using HiTrap Desalting column (Sigma-Aldrich cat # GE17-1408-01) equilibrated with 50mM phosphate buffer pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentrations of the samples were determined by the BCA assay kit (Sigma-Aldrich, USA). Digitonin (Calbiochem ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were denatured by addition of 2X Laemmeli buffer/10% β-mercaptoethanol (Bio-Rad/Sigma, respectively) to samples and subjection of samples to boiling for 2 min at 95°C ...
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bioRxiv - Microbiology 2022Quote: ... 400 μg of total protein lysates were incubated overnight with Anti-FLAG M2 Magnetic Beads (Sigma) or control G-coupled magnetic beads ...
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bioRxiv - Molecular Biology 2022Quote: ... These protein samples were separated using 10% SDS–PAGE and transferred to nitrocellulose membranes (Millipore, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... 75 μg of protein was precipitated with an equal volume of 20% trichloroacetic acid (TCA, Sigma) for 16 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... to the hen’s egg protein ovalbumin (OVA) (20 mg/0.5 mL PBS; grade V; Sigma-Aldrich) using cholera toxin (CT ...
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bioRxiv - Plant Biology 2022Quote: ... Total protein loading was visualized by staining nitrocellulose membranes with Ponceau S solution (Sigma-Aldrich, P7170).
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Serum proteins were first precipitated by addition of 10% (w/v) trichloroacetic acid (TCA; Sigma, 49010) and incubated at -20°C for up to 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred (300 mA, 3h) onto ethanol-activated Immobilon-P PVDF membrane (Millipore) before blocking with 3% Bovine Serum Albumin in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred to Immobilon-P polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Burlington, MA, USA) using a semidry blotting system (Nihon Eido ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then transferred to a methanol (MeOH)-activated polyvinylidene fluoride (PVDF) membrane (Immobilon-P; Millipore) using a wet tank electro-blotting apparatus (Mini Trans-Blot ...
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bioRxiv - Plant Biology 2023Quote: Nuclear proteins were extracted from frozen poplar stem using the CellLytic™ PN kit (Sigma, USA) according to the manufacturer’s instruction ...