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Citations for Millipore Sigma :
7501 - 7550 of 10000+ citations for Anti PIGR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-rabbit secondary antibody conjugated with horse radish peroxidase enzyme was used in the ratio of 1:10000 (Sigma Aldrich, USA) and blots were visualized using chemiluminescent substrate as described above.
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... in PBS (BSA/PBS-T) for 1 hour prior to washing with PBS and incubation with primary anti-TH rabbit polyclonal antibody (EMD Millipore) at 1:300 BSA/PBS-T overnight at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... After washing the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, A2179) in PBST ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Systems Biology 2024Quote: The anti-FLAG HRP antibody (clone M2, cat#: A8592) and anti-FLAG affinity gel (clone M2, cat#: A2220) were purchased from Sigma-Aldrich. The anti-β-Actin antibody (clone# ...
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bioRxiv - Neuroscience 2023Quote: ... Processing of brain and brain sections for TH immunohistochemistry was similar to the protocol described above except for primary (rabbit anti-TH antibody 1/200, Millipore ab152) and secondary (Alexa Fluor 555-conjugated donkey anti-rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were blocked with 5% BSA in 0.1% Triton X-100 for 2 hours and the same solution was used for overnight primary antibody dilution at 4°C with anti-FLAG M2 (Sigma, F1804) 1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were blocked with 5% BSA in 0.1% Triton X-100 for 2 hours and the same solution was used for overnight primary antibody dilution at 4°C with anti-FLAG M2 (Sigma, F1804) 1:300 ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation with an anti-EMILIN-1 rabbit polyclonal antibody (x400, HPA002822; Sigma Aldrich, Saint Louis, MO, USA) in Dako REAL Antibody Diluent at 4°C for 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Reaction samples were then resolved in 10% SDS-PAGE followed by Western blot analysis using anti-acetylated lysine antibody (Sigma, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates (13,000 rpm, 10 min, 4°C) were incubated with 35 µl anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) for 30 min at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 s ON/30-50 s OFF) and used for IP with anti-FLAG antibody (FLAG M2 magnetic beads, Sigma-Aldrich). For ChIP-seq ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated for 48 hr at 4°C with mouse anti-TH antibody (1:2,000; Millipore, Burlington, MA) in 0.1 M PBS containing 2% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Neuroscience 2023Quote: Single nuclei were isolated by fluorescence-activated nuclear sorting (FANS) using an anti-NeuN antibody as a neuronal nuclei marker (Millipore, MAB377). Nuclei were lysed on ice in alkaline conditions ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound NS5A antibody was detected after two washes with PBS-T by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse antibody (A4416, Sigma-Aldrich) diluted 1:300 in PBS-T ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C: mouse anti-FLAG M2 (1:500; Sigma; F1804), rabbit anti-PEX14 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated for 1h at room temperature with the secondary antibody anti-mouse IgG peroxidase-conjugated (1/500; A5906, Sigma-Aldrich). The revelation was performed using Pierce™ ECL Plus Western Blotting Substrate (32312 ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were stained for neurons using NeuN (anti-NeuN antibody, clone A60, biotin conjugated, Millipore Cat. No. MAB377B, 1500 dilution) and visualized using 3,3′-Diaminobenzidine (DAB) ...
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bioRxiv - Plant Biology 2024Quote: ... 2003) and a goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (1:4000, Chemicon®, Sigma-Aldrich, www.sigmaaldrich.com, #AP307P). Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Probed membrane was washed with TBS-T thrice before incubating with alkaline phosphatase-conjugated anti-mouse secondary antibody (1:10000 dilution, Sigma, USA) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... we blocked membranes with 5% milk in PBS for 1 hour at room temperature and probed overnight with rocking at 4°C with the following primary antibodies: mouse anti-FLAG clone M2 (1/1000 dilution, Sigma # F1804), mouse anti-HA clone 16B12 (1/3000 dilution for HEK-293 cell lysates ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with 5% BSA at room temperature for 1 hour and incubated with anti-FLAG M2-peroxidase antibody (Sigma-Aldrich) at room temperature for another hour ...
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bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
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bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
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bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Cell Biology 2024Quote: ... with 200 μl of 15 μg/ml anti-CD63 antibody (Cosmo Bio, SHI-EXO-M02) or mouse IgG2a isotype control (Millipore, MABF1080Z) in PBS containing 0.02% Tween 20 ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Biophysics 2024Quote: ... Immunostaining was performed as previously described [84] using the following antibodies: Mouse anti-Rac1 (Millipore Cat#05-389-25UG, 1:200), Rabbit anti-RhoA (Proteintech Cat#10749-1-AP ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were incubated overnight in a humidified chamber at room temperature with the following antibodies diluted with 10% normal goat serum as follows: anti-Flag (Sigma, F1804) 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Biophysics 2024Quote: ... isolated myofibrils were incubated for 1.5 hrs at 4°C on a rotator with 11 nM anti-α-actinin mouse antibody (A7811; Sigma-Aldrich), 5.5 nM Alexa-488 goat anti-mouse IgG (A11001 ...
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bioRxiv - Neuroscience 2024Quote: ... dorsal and ventral transverse slices were incubated with the following primary and secondary antibody combinations using the protocol described: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then rinsed 3 times for 10 minutes in PBS and incubated overnight at 4°C in a mix of the primary antibodies (mouse anti-S100β, Sigma Aldrich #S2532 ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...