Labshake search
Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for Anti PIGR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
-
bioRxiv - Microbiology 2022Quote: ... was loaded alongside the pulldown samples which were resolved on 10% SDS polyacrylamide gels and subject to western blotting using anti-GST antibody (Sigma G7781). Membranes were developed with an ECL plus Chemiluminescent kit (GE Healthcare ...
-
bioRxiv - Plant Biology 2022Quote: ... 15 cycles of 30 s on and 30 s off) and immunoprecipitated using an anti-GFP antibody (Sigma-Aldrich; Merck KGaA). The immunoprecipitated and purified DNA was used for quantitative real-time PCR ...
-
bioRxiv - Neuroscience 2022Quote: ... brains were first cut into 50 um slices and store at 4C in .1% sodium azide PBS before undergoing NeuN staining procedures using Alexa Fluor 488 Conjugate ABN78A4 Anti-NeuN (rabbit) antibody (Sigma-Aldrich). Slices were washed 3 times for 10 minutes with 1x PBS and pre-incubated in 10% Horse Serum and 0.3% Triton-X-100-PBS with 1% BSA for 1 hour ...
-
bioRxiv - Plant Biology 2022Quote: ... Total NUC/GEN polypeptide abundance was detected by probing duplicate blots with a mouse anti-GFP antibody (#11814460001; Sigma Aldrich (Roche), Darmstadt ...
-
bioRxiv - Neuroscience 2023Quote: ... Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, 1:10.000 dilution) for 1 h at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... was used as a substrate for in situ hybridization and immunolabeling was carried out with primary antibodies anti-GABA (1:500, Sigma–Aldrich), anti-GFP (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed and subjected to Anti-DIG-AP antibody by using DIG Wash and Block Buffer Set (Sigma, #11585762001) and immonological detection of the membrane was conducted by CDP-Star chemiluminescent substrate included in the northern blotting kit.
-
bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
-
bioRxiv - Biochemistry 2022Quote: ... 2.5 μL time points were taken and quenched with 2 mM EDTA then brought to 20 μL final volume for detection using 1:500 dilution anti-H3K4me3 antibody (05-745R, EMD Millipore), 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082) ...
-
bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissue was permeabilized/blocked in a solution containing 1% Triton-X and 10% NGS in PBS for 30 min and then incubated with an anti-HNE-Michael adducts rabbit polyclonal antibody (1:150 dilution, EMD Millipore) in a 10% NGS ...
-
bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
-
bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS and incubated with secondary antibodies at 1:500 dilution (goat anti-rabbit Alexa 594, Sigma Aldrich,) for 1h at room temperature ...
-
bioRxiv - Plant Biology 2024Quote: ... and incubated overnight in the same solution at 4°C with a 6000 X dilution of anti-HA-peroxidase antibodies (Sigma-Aldrich) in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Systems Biology 2024Quote: The anti-FLAG HRP antibody (clone M2, cat#: A8592) and anti-FLAG affinity gel (clone M2, cat#: A2220) were purchased from Sigma-Aldrich. The anti-β-Actin antibody (clone# ...
-
bioRxiv - Plant Biology 2024Quote: ... 2003) and a goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (1:4000, Chemicon®, Sigma-Aldrich, www.sigmaaldrich.com, #AP307P). Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... Probed membrane was washed with TBS-T thrice before incubating with alkaline phosphatase-conjugated anti-mouse secondary antibody (1:10000 dilution, Sigma, USA) or goat anti-rabbit IgG (H+L ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bound NS5A antibody was detected after two washes with PBS-T by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse antibody (A4416, Sigma-Aldrich) diluted 1:300 in PBS-T ...
-
bioRxiv - Microbiology 2024Quote: ... correct production of the protein of interest was checked by Western Blot of HEK293T-transfected cells using an anti-Flag antibody (Sigma-Aldrich).
-
bioRxiv - Molecular Biology 2024Quote: Antibodies used for immunoblotting in this study include rabbit polyclonal anti-GFP (ab290, Abcam, 1:2,500 or Sigma, G1544, 1:1000), mouse monoclonal anti-GFP (JL8 ...
-
bioRxiv - Neuroscience 2024Quote: ... for one hour before overnight incubation at 4℃ with one of the following primary antibodies: anti-NG2 (1:200; Millipore AB5320), anti-Laminin (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... Potential contamination among subcellular fractions was inspected by the presence of nucleus-specific marker Histone H3 on Western blot with human anti-Histone H3 antibody (ab1791) and mitochondria-specific activity of succinate dehydrogenase using the Succinate dehydrogenase activity colorimetric Assay (Sigma Aldrich), both performed according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
-
bioRxiv - Neuroscience 2024Quote: ... iBECs were cultured in Transwell inserts with 3.0 µm pores and exposed to FITC-conjugated 150 kDa dextran (0.5 mg/ml) or anti-TDP-43 antibody (1 µM, Sigma, Cat#T1705) and 10 µl of phospholipid-shelled microbubbles with octafluoropropane gas core prepared in-house as described in (40) ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in blocking solution (5% normal donkey serum and 0.3% Triton X-100 in PBS) for 30 minutes and then incubated in rabbit anti-TH polyclonal antibody (Millipore, Cat: AB152) diluted 1:1000 in 0.3% Triton X-100 in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... and 0.25% Triton X-100 for 30 min at RT and subsequently incubated in 0.1% Triton X-100 and 1% NS in PB with the following primary antibodies: mouse monoclonal anti-NeuN (1:1000; Millipore, #MAB377), anti-SMI32 (specific for non-phosphorylated neurofilament H ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
-
bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP ...
-
bioRxiv - Developmental Biology 2024Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed five times with five-minute incubations in PBST and then incubated overnight in primary antibody solution containing anti-acetylated alpha-tubulin (1:500; Sigma, T6793) in PBST plus 20% NGS ...
-
bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
-
bioRxiv - Immunology 2024Quote: ... Viral nucleoprotein (NP) was detected by ELISA as described using a 1/1,000 dilution of the anti-NP antibody (Millipore cat. #MAB8257) and a 1/2,000 dilution of the horseradish peroxidase-labeled goat anti-mouse IgG (SeraCare cat ...
-
bioRxiv - Microbiology 2024Quote: ... Filters were scanned by densitometry and the relative phosphorylation level of each MAPK member was determined relative to anti α-tubulin antibody T9026 (Sigma) loading control ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates (13,000 rpm, 10 min, 4°C) were incubated with 35 µl anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) for 30 min at 4°C with rotation ...
-
bioRxiv - Cell Biology 2023Quote: ... Reaction samples were then resolved in 10% SDS-PAGE followed by Western blot analysis using anti-acetylated lysine antibody (Sigma, USA).
-
bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Serial sections were stained for neurons using NeuN (anti-NeuN antibody, clone A60, biotin conjugated, Millipore Cat. No. MAB377B, 1500 dilution) and visualized using 3,3′-Diaminobenzidine (DAB) ...
-
bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
-
bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated for 1h at room temperature with the secondary antibody anti-mouse IgG peroxidase-conjugated (1/500; A5906, Sigma-Aldrich). The revelation was performed using Pierce™ ECL Plus Western Blotting Substrate (32312 ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C: mouse anti-FLAG M2 (1:500; Sigma; F1804), rabbit anti-PEX14 (1:500 ...
-
bioRxiv - Immunology 2023Quote: ... Tapasin was detected in the lysates following 12% SDS-PAGE and western transfer using anti-tapasin antibody (MABF249 from Sigma-Aldrich).
-
bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
-
bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...