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Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for Anti PIGR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... For KIF18A localization analyses the following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (MAB1864; Sigma Aldrich), rabbit anti-KIF18A 1:100 (A301-080A ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Genetics 2019Quote: ... 0.1%SDS and 1% Tween 20 and re-stained for the loading using the anti-glucose-6-phosphate dehydrogenase antibody (Millipore, cat #A9521). Blots were again imaged on ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog # 11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) or 5ug Normal Mouse IgG Polyclonal Antibody (MERCK ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were analysed using 4-12% Bis-Tris polyacrylamide gels in MOPS buffer followed by Coomassie staining or western blot using the following antibodies: Mouse anti-FLAG M2 (Sigma, 1:10,000). RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... ovaries were incubated overnight at 4°C under agitation with the primary antibody (anti-pTyr (4G10) (05-321, Millipore Sigma, 1:40), anti-pERM (3141S ...
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bioRxiv - Developmental Biology 2022Quote: ... and cultures were incubated overnight at 4°C with primary antibodies diluted in TBS/10% goat serum: mouse anti-β-tubulin-III (Sigma #T8660) or mouse anti-MAP2 (Sigma ...
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Each strain to be tested was then washed twice with sterile 1x PBS and adjusted to a concentration of ~2×105 cells/mL in macrophage growth media containing 1 μg/mL of the anti-GXM antibody 18B7 (Sigma Cat# MABF2069). Cells were opsonized in this media for 1h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Sixty μl of Dynabeads Protein G were incubated for 1h30 at 4°C under agitation with 16 μg of the anti-Ago2 antibody (Sigma-Aldrich, SAB4200085) in 1X PBS containing 0.1% of Tween 20 ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were then rinsed 3 times in 0.3% triton X-100/PBS and incubated for one hour at room temperature with the secondary fluorescent antibody (Donkey, anti-goat, Alexa fluor 488 secondary, Sigma-Aldrich - USA) concentration (1:1000) ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore, MAB1574, 1:5,000).
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bioRxiv - Neuroscience 2022Quote: ... Larvae were washed three times in 0.1% PBST and incubated with secondary antibodies: anti-mouse Alexa Flour 647, 1:100 (Invitrogen, #28181. Stained larvae were mounted using DAPI Fluoroshield (Sigma-Aldrich, #F6182). Images were collected on a Nikon A1 eclipse Ti confocal microscope.
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bioRxiv - Molecular Biology 2023Quote: ... ChIP experiments were performed basically in accordance with the above procedure using 1 µg of an anti-H4K16ac antibody (Millipore, 07-329) and 2.4 µg of an anti-H2A.Z antibody (Kudo et al ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated for 1 hour at room temperature with a goat anti-mouse alkaline phosphatase-conjugated secondary antibody (dilution 1: 5000; Sigma-Aldrich, USA) and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skim milk in TBST and target proteins were detected with ANTI-FLAG® M2 antibody (Sigma-Aldrich) and anti-α-tubulin (SantaCruz).
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bioRxiv - Neuroscience 2022Quote: ... immunohistochemical examination of all surgical brain specimens was performed using the following panel of antibodies: mouse monoclonal anti-NeuN (clone A-60, Millipore Cat#MAB377), mouse monoclonal anti-GFAP (clone 6F2 ...
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... mid-sagittal cerebellar vibratome sections were blocked and permeabilized with 10% normal goat serum in PBS containing 0.2% Triton X-100 and immunostained with a mouse anti-Calbindin D28k antibody (Sigma-Aldrich, 1:1000) followed by incubation with Alexa Fluor 555-labeled goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog #11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution: anti-smooth muscle actin (SMA) 1:500 (Sigma, A2547), anti-GFP 1:200 (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... Triton-X 100 in 1x PBS) for 10 min after which it was incubated with primary antibody solution (anti-alpha-tubulin, #T5168, Sigma-Aldrich, Germany) in IF buffer diluted 1:500 for 1.5 h at room temperature while shaking followed by washing thrice with 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated overnight at 4°C with mouse anti-TH antibody (1:8000 dilution, T2928, Sigma-Aldrich, St. Louise, MO, USA), 1% NHS ...
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bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and developed by detecting bound IgG or IgA with appropriate fluorochrome-labelled secondary antibodies: 1 µg/ml goat anti-human IgG-Cy3 (Sigma, Cat# C2571) or goat anti-human IgA-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... The membranes that were loaded with fetal membrane and uterine tissue lysates were re-probed for 1 h at room temperature with a mouse anti-β-actin (ACTB) monoclonal antibody (Cat# A5441, Sigma-Aldrich). Then ...
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 20 000 rpm for 30 min at 4 °C and supernatants were analyzed by Western Blot using monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody (Sigma-Aldrich, A8592).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed again and incubated for 1 hour min with secondary FITC-conjugated goat anti-rabbit antibody (Sigma-Aldrich; 1:100) and TRITC-conjugated phalloidin ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells on coverslips were blocked with blocking solution (2% BSA/PBST) for 30 min and incubated with the primary antibodies anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were diluted in 10% goat serum and incubated for 18 hours at 4°C with laminin rabbit anti-mouse antibody (1:250, Sigma-Aldrich, L9393). Sections were then incubated for 1 hour at room temperature in their corresponding goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... the treated cells were performed using anti-chicken IgY (IgG) (whole molecule)-FITC antibody produced in rabbit (Sigma–Aldrich, dilution 1:640) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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Characterization of pediatric porcine pulmonary valves as a model for tissue engineered heart valvesbioRxiv - Bioengineering 2023Quote: ... The co-stain dye was prepared from a 1:50 dilution of anti-α-SMA CY3 conjugated primary antibody (Sigma, cat #C6198) and 1:50 dilution of anti-vimentin Alexa Fluor™ 488 conjugated primary antibody (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 µl wash buffer containing 10 µl 1 µg/µl anti-rabbit secondary antibody raised in guinea pig (Sigma-Aldrich, SAB3700889) and 1 U/µl RNase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... The grid was blotted with filter paper and floated on a 20 µl drop of primary anti-HA antibody (Sigma-Aldrich #H6908) diluted 1:500 in blocking buffer for 1 h in a closed ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins of the Co-IP were analyzed by Western Blot with the Monoclonal ANTI-FALG® M2 antibody produced in mouse (Sigma Aldrich), proteins of inputs were analyzed by Western Blot with the Anti-GFP from mouse (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...