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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 2-Deoxy-Glucose (2-DG, Sigma Aldrich).
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bioRxiv - Cancer Biology 2021Quote: Native ChIP-qPCR was performed on post-mortem tissue using antibodies against H3K27ac (2 µl, cat# 07360, Millipore Sigma), H3K27me3 (1 µg ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were permeabilised and blocked with a solution of 2% bovine serum albumin and 0.2% triton X-100 in PBS before incubation with primary antibodies (anti-HA, Sigma, 1:1000 and rabbit anti-TOM40 ...
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bioRxiv - Molecular Biology 2022Quote: ... equivalent to 20 μl of the original slurry were resuspended in PBS with 0.02 % Tween-20 and coupled with 2 μl of Anti-FLAG M2 antibody (Sigma) rotating for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and then incubated for 2 hours at room temperature in host-specific HRP-conjugated secondary antibody (1:10,000, Sigma) in TBS-T containing 5% skim milk ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... blocked with 0.5% bovine serum albumin and 0.2% triton X-100 for 2 hours and incubated with primary antibody (rabbit anti-Tyrosine hydroxylase, Millipore) overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... each of the anti-MuSK clones was combined with 1.3-1.4x molar excess of the b12 antibody and 75 mM 2-Mercaptoethylamine-HCl (Sigma-Aldrich) for five hours at 31°C (Labrijn et al. ...
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bioRxiv - Genomics 2021Quote: ... After washing nuclei-bound beads were resuspended in 50 µl Buffer 2 with 0.5 µl antibody (H3K4me3 from Millipore, 04-745 ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... purified resting B cells were cultured at a density of 1.5-2× 106/ml in B cell culture medium supplemented with 25mg anti-IgM antibody (Millipore) and 2ng/ml of IL-4 (PeproTech ...
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bioRxiv - Cell Biology 2022Quote: ... Postnuclear supernatants were incubated for 2 hours with pre-equilibrated 20ul anti-HA antibody magnetic beads slurry (Sigma-Aldrich). After three 10min-washes in 0.2% DMNG washing buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Postnuclear supernatants were incubated for 2 hours with pre-equilibrated 30ul anti-HA antibody magnetic beads slurry (Sigma-Aldrich). After three 15min-washes in RIPA buffer on a rotating wheel at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Every 10 μg of chromatin was incubated with 2 µg of mouse M2 anti-FLAG monoclonal antibodies (Sigma-Aldrich) overnight at 4° C on a nutator ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS plus 0.1% triton (PBST) for 2 hours and then transferred to 1:1000 TH anti-rabbit primary antibody (Millipore) with 1% NDS in PBST overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were applied for 2 hours at room temperature and counterstained with DAPI (Sigma Aldrich D9542, 1:10000) for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with a poly-dT oligonucleotide coupled with Cy-2 and a murin anti-FUS primary antibody (Sigma Aldrich) as previously described.
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... sections adjacent to those used for IR measurements were stained with the anti-amyloid MOAB-2 antibody (Sigma-Aldrich) at the University of Alabama at Birmingham pathology core research lab ...
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bioRxiv - Cancer Biology 2023Quote: ... the antibody-linker solution was washed once using a 30 kDa Amicon Ultra-2 centrifugal filter (UFC203024, Sigma-Aldrich). Annealed DNA oligonucleotides with a 5′ azide modification (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated for 2 h at room temperature with a monoclonal anti-FLAG antibody (Sigma Aldrich, F1804) diluted 1:1,000 in blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized by isofluorane (Piramal) or avertin (2, 2, 2-Tribomoethanol, Sigma) and transcardially perfused by phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (benzyl-GalNAc; Sigma), for three days ...
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bioRxiv - Cancer Biology 2022Quote: ... After 2 hours medium was replaced with 2 mL medium containing 2 % FBS and 2 mg/mL polybrene (Sigma). After an hour this medium was removed and replaced with 2mL of medium containing 5x 106 IU of lentiviral vector ...
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bioRxiv - Cell Biology 2020Quote: ... cells were blocked for nonspecific binding by using Block – a 5% solution of bovine serum albumin (BSA, Sigma A2153) in PBS – for 30 minutes at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... according to the following protocol: 600 µl of binding buffer and 5 µl 3M sodium acetate (Sigma-Aldrich, 71196) were added to the synthesis product ...
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bioRxiv - Cell Biology 2020Quote: ... Unspecific binding in frozen and paraffin sections was blocked with 5% normal horse serum in 0.05% Triton X-100 (Sigma) for 30 min RT ...
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bioRxiv - Biophysics 2019Quote: ... lyophilized protein was dissolved in sodium phosphate buffer (pH 7.4) and filtered using 0.22 μm low protein binding membranes (Millex-GP, Merck Millipore, Germany). The protein concentration was determined by the measurement of absorbance at 277 nm using the extinction coefficient 5960 cm-1M-1.
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bioRxiv - Immunology 2019Quote: ... The binding was revealed with 0.5 mg/mL o-phenylenediamine dihydrochloride substrate (OPD) (Sigma-Aldrich, St Louis, MO, USA), and Optical Density (OD ...
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bioRxiv - Biochemistry 2019Quote: ... The pure 50S pellet was dissolved in Buffer A and filtered using 0.22 µm low-binding Durapore PVDF filters (Millipore).
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bioRxiv - Microbiology 2020Quote: ... BBMV were centrifuged and resuspended in binding buffer (20 mM Tris, 150 mM NaCl, 1 mM MnCl2, pH 7.4, 0,04% Blocking reagent from Sigma Aldrich). Competition binding experiments were conducted by incubating 1.4 μg of BBMV protein with 0.65 nM 125I-Vip3Aa in a final volume of 0.1 ml of binding buffer for 90 min at 25°C in the presence of increasing amounts of unlabeled Vip3Aa ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Bioengineering 2020Quote: ... single-polymer and IPN hydrogels were incubated with an HA binding protein derived from bovine nasal cartilage (EMD Millipore) as previously described by Suri & Schmidt [31] ...
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bioRxiv - Neuroscience 2021Quote: ... Viral supernatant was transferred into a new sterile 50 mL tube and passed through a low protein binding 0.45 μm filter (SE1M003M00, Millipore) to exclude any cells still present in the supernatant ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding was blocked using 5% of normal goat serum (16210064, Life technology) with 2.5% BSA (10735078001, Sigma) for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding was blocked using 5% of normal goat serum (16210064, Life technology) with 2.5% BSA (10735078001, Sigma) for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... and posteriorly permeabilized and blocked against nonspecific binding using a solution of 3% bovine serum albumin (BSA) (Sigma-Aldrich) and 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifuged at 20000g for 15mins and filtered through a low-protein binding 0.45µm PVDF membrane syringe filter (Millipore, #SLHVX13NL), the extracts were incubated with 30µl mouse anti-HA agarose beads (clone HA-7 ...