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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS plus 0.1% triton (PBST) for 2 hours and then transferred to 1:1000 TH anti-rabbit primary antibody (Millipore) with 1% NDS in PBST overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with a poly-dT oligonucleotide coupled with Cy-2 and a murin anti-FUS primary antibody (Sigma Aldrich) as previously described.
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were applied for 2 hours at room temperature and counterstained with DAPI (Sigma Aldrich D9542, 1:10000) for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... sections adjacent to those used for IR measurements were stained with the anti-amyloid MOAB-2 antibody (Sigma-Aldrich) at the University of Alabama at Birmingham pathology core research lab ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated for 2 h at room temperature with a monoclonal anti-FLAG antibody (Sigma Aldrich, F1804) diluted 1:1,000 in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... the antibody-linker solution was washed once using a 30 kDa Amicon Ultra-2 centrifugal filter (UFC203024, Sigma-Aldrich). Annealed DNA oligonucleotides with a 5′ azide modification (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized by isofluorane (Piramal) or avertin (2, 2, 2-Tribomoethanol, Sigma) and transcardially perfused by phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (benzyl-GalNAc; Sigma), for three days ...
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bioRxiv - Cancer Biology 2022Quote: ... After 2 hours medium was replaced with 2 mL medium containing 2 % FBS and 2 mg/mL polybrene (Sigma). After an hour this medium was removed and replaced with 2mL of medium containing 5x 106 IU of lentiviral vector ...
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bioRxiv - Cell Biology 2020Quote: ... cells were blocked for nonspecific binding by using Block – a 5% solution of bovine serum albumin (BSA, Sigma A2153) in PBS – for 30 minutes at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... according to the following protocol: 600 µl of binding buffer and 5 µl 3M sodium acetate (Sigma-Aldrich, 71196) were added to the synthesis product ...
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bioRxiv - Cell Biology 2020Quote: ... Unspecific binding in frozen and paraffin sections was blocked with 5% normal horse serum in 0.05% Triton X-100 (Sigma) for 30 min RT ...
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bioRxiv - Biophysics 2019Quote: ... lyophilized protein was dissolved in sodium phosphate buffer (pH 7.4) and filtered using 0.22 μm low protein binding membranes (Millex-GP, Merck Millipore, Germany). The protein concentration was determined by the measurement of absorbance at 277 nm using the extinction coefficient 5960 cm-1M-1.
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bioRxiv - Immunology 2019Quote: ... The binding was revealed with 0.5 mg/mL o-phenylenediamine dihydrochloride substrate (OPD) (Sigma-Aldrich, St Louis, MO, USA), and Optical Density (OD ...
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bioRxiv - Biochemistry 2019Quote: ... The pure 50S pellet was dissolved in Buffer A and filtered using 0.22 µm low-binding Durapore PVDF filters (Millipore).
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bioRxiv - Microbiology 2020Quote: ... BBMV were centrifuged and resuspended in binding buffer (20 mM Tris, 150 mM NaCl, 1 mM MnCl2, pH 7.4, 0,04% Blocking reagent from Sigma Aldrich). Competition binding experiments were conducted by incubating 1.4 μg of BBMV protein with 0.65 nM 125I-Vip3Aa in a final volume of 0.1 ml of binding buffer for 90 min at 25°C in the presence of increasing amounts of unlabeled Vip3Aa ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Bioengineering 2020Quote: ... single-polymer and IPN hydrogels were incubated with an HA binding protein derived from bovine nasal cartilage (EMD Millipore) as previously described by Suri & Schmidt [31] ...
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bioRxiv - Neuroscience 2021Quote: ... Viral supernatant was transferred into a new sterile 50 mL tube and passed through a low protein binding 0.45 μm filter (SE1M003M00, Millipore) to exclude any cells still present in the supernatant ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding was blocked using 5% of normal goat serum (16210064, Life technology) with 2.5% BSA (10735078001, Sigma) for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding was blocked using 5% of normal goat serum (16210064, Life technology) with 2.5% BSA (10735078001, Sigma) for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... and posteriorly permeabilized and blocked against nonspecific binding using a solution of 3% bovine serum albumin (BSA) (Sigma-Aldrich) and 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifuged at 20000g for 15mins and filtered through a low-protein binding 0.45µm PVDF membrane syringe filter (Millipore, #SLHVX13NL), the extracts were incubated with 30µl mouse anti-HA agarose beads (clone HA-7 ...
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bioRxiv - Plant Biology 2019Quote: ... One hundred micro litter of supernatant was incubated with 1 μl of 0.1 mM coelenterazine-h in a 96-well plate for 30 min to facilitate binding between coelenterazine-h (Sigma) and aequorin ...
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bioRxiv - Genetics 2021Quote: ... Reversal of TetR fusion protein binding was achieved by addition of 1 μg/ml Doxycycline (final concentration / Sigma-D9891) to mESC culture medium.
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bioRxiv - Molecular Biology 2021Quote: The Toxoid sequence pltB_pltA(E133A)_cdtB(H160Q)-6xHis and its corresponding non-binding control pltB(S35A)_pltA_cdtB-6xHis in the bacterial expression vector pET28b (Novagen) was kindly provided by Jorge Galan and described elsewhere ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ng/mL of heparin-binding epidermal growth factor (HB-EGF) (SRP6050-10UG, Sigma-Aldrich, St Louis, MO, USA), 20 ng/mL of EGF and/or pre-treated with 2 μM gefitinib for 1h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatant was passed through a 1.2 µM filter before batch binding to ANTI-FLAG M2 affinity resin (Sigma) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were collected at 42-48 h after transfection and filtered through a 0.45 um low-protein binding filter (Millipore). Patient-derived TCL were infected with lentiviral supernatants containing polybrene (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Neuroscience 2024Quote: ... Non-specific binding was defined by competition with non-radioactive GTPγS (10 µM) (Sigma-Aldrich, St. Louis, MO, USA) in another section ...
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bioRxiv - Physiology 2024Quote: Cells were stained with the DNA-binding dyes propidium iodide (PI) and Hoechst 33342 (10 µg/mL, Sigma-Aldrich) to count apoptotic cells by fluorescent microscopy ...
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bioRxiv - Cancer Biology 2024Quote: ... conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Immunology 2020Quote: ... in a bead-to-cell ratio of 0.5:1 in simultaneous presence or absence of 2 mM 2-Fluoro-2-deoxy-D-glucose (2-FDG) (Sigma, F5006) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with avertin (2, 2, 2-tribromoethanol, Sigma Aldrich, St. Louis, MO) or isoflurane for stereotaxic surgery (Model 1900 Stereotaxic Alignment System ...
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bioRxiv - Immunology 2023Quote: ... After general anesthesia with i.p.-administered Avertin (2, 2, 2-tribromoethanol, Sigma-Aldrich #T48402) the blood was collected with heparinized capillaries (Fisher Scientific™ #22-260950 ...
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bioRxiv - Immunology 2023Quote: ... 10 µM N-acetylcysteine (NAC) or 2 mM 2-desoxyglucose (2-DG, both Sigma) were added during cultivation ...
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bioRxiv - Biochemistry 2023Quote: ... Irgacure 2959 (2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone) were purchased from Sigma Aldrich for preparation of crosslinked polymer.
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...