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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... with PBS +0.05% Tween20 and bound FLAG-ACE-2 was detected with HRP-conjugated anti-FLAG antibody (1:20,000, Sigma cat# A8592) and peroxidase substrate (KPL ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... After incubating in primary antibodies for two hours and DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride, Sigma Aldrich, 10,236,276,001 Roche) for the last ten minutes ...
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bioRxiv - Biophysics 2020Quote: ... both paraformaldehyde and methanol fixed cells are incubated with primary antibody diluted in the antibody dilution buffer (PBS supplemented with 5% FBS, 2% bovine serum albumin and 0.1% saponin (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 2 h with horseradish peroxidase (HRP)-conjugated secondary antibody (12-341 Rabbit anti-chicken RAC-HRP, Sigma-Aldrich) diluted 1:15000 in BSA-TBST ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then incubated with primary antibodies at RT for 2 hrs before proceeding with Duolink PLA (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... fiber type IIb) (hybridoma supernates, Developmental Studies Hybridoma Bank) supplemented with rabbit polyclonal anti-laminin antibody (2 μg/ml, Sigma) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were immunoprecipitated with 2 μg of IgG (negative control) or Smad3 antibody in coimmunoprecipitation reagents (17-195, Millipore). Semi-quantitative and quantitative PCR were performed to amplify α-SMA or Col1A promoter regions containing SBE.
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were washed 5 times in PBT and incubated for 2h at RT with secondary antibodies and 4’,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma) (1µg/mL) ...
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bioRxiv - Plant Biology 2020Quote: ... were isolated from 2-week-old dPPRrbcL-1 or Col-0 plants and incubated with monoclonal anti-HA antibodies (Sigma). IgGs were captured with Protein A DynaBeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 1×106 yeast cells were incubated in staining buffer (phosphate buffered saline + 0.5% BSA + 2mM EDTA) containing anti-Flag fluorescein isothiocyanate antibody (2 μg/mL; clone M2, Sigma-Aldrich) and the probes for 30 minutes at RT in darkness prior to washing 2 times in ice cold staining buffer ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-PP1α antibody (2 μg) was added to the supplied spin column (Catch and Release Immunoprecipitation Kit; cat no. 17-500; Millipore) along with the cell lysates (500 mg ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then washed in PBS and incubated with the appropriate secondary antibody diluted in PBS containing 2% horse serum and 0.1% triton X-100 (Sigma-Aldrich) with Donkey anti-rabbit Alexa488 (1:200 Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... The antibodies used in this study were as follows: laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), MYOD1 (mouse monoclonal ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with HRP-conjugated goat anti-rabbit antibody (1:10,000, Sigma-Aldrich, St. Louis, MO, USA). The signal was visualized by chemiluminescent detection using ECL.
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Molecular Biology 2023Quote: ... the elutions containing ORC (typically 2-5) were pooled and incubated with 40 µl of equilibrated Anti-V5-antibody-coupled Agarose Affinity Gel (Sigma) for 30 min at 4°C with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Goat anti-mouse IgG-HRP or Goat anti-rabbit IgG-HRP, Sigma) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were rinsed with PBS and nuclei were labeled using DAPI (4’6-diamidino-2-phenylindole; Sigma, St. Louis, MS) in PBS (1:10,000 of 3mg/mL DAPI stock ...
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by washes in PBST and incubated with secondary antibody for 2 hr at room temperature and nuclei were counter-stained with DAPI (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Immunology 2021Quote: ... was coupled to sepharose in the presence of binding buffer (150 mmol/L sodium chloride, 50 mmol/L sodium bicarbonate, pH 8.3; sodium chloride: Sigma-Aldrich, cat ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by two hours blocking of unspecific binding sites using blocking buffer composed of 10% normal goat serum (NGS, Sigma), 1% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing medium was collected 46 and 65 hours post transfection and was filtered through 0.45 μM Millipore low-protein binding filter units (Millipore SCHVU02RE). Viral supernatant was concentrated by first using low speed centrifugation through 100 kDa MW cutoff Millipore Centricon 70 Plus cartridges to condense volume to < 4mL ...
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bioRxiv - Cell Biology 2019Quote: ... and bound (50% of total binding) samples were resolved by SDS-PAGE and immunoblotted with FLAG (1:2,000; Sigma F1804), Myc (1:3,000 ...
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bioRxiv - Biochemistry 2020Quote: QnrB1/DNA binding competition assays were performed as follows: 90 bp Cy5-labelled oligonucleotide encompassing the strong gyrase binding site from plasmid pBR322 was ordered from Sigma and annealed with an antisense unlabelled oligo by heating to 99°C and gradual cooling ...
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bioRxiv - Microbiology 2020Quote: ... Loaded proteins were washed with 300 μL Binding Buffer (90% methanol and 10% triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich, #T7408), adjusted to pH 7.1 using phosphoric acid ...
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bioRxiv - Microbiology 2020Quote: The Congo red binding (CRB) ability of the APEC isolates was determined using agar plates supplemented with 0.003% CR dye (Sigma, USA) and 0.15% bile salts ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mg/L avidin and 6 mM MgCl2 were added to the supernatant to improve the Strep tag binding efficiency with Strep-Tactin beads (Novagen). Next ...
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bioRxiv - Microbiology 2020Quote: ... Elution of proteins was done by adding 100 µl of binding buffer supplemented with 20 mM IP6-sodium salt (Sigma) and then incubating overnight at 4°C under constant mixing ...
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bioRxiv - Cancer Biology 2020Quote: ... was coupled to sepharose in the presence of binding buffer (150 mmol/L sodium chloride, 50 mmol/L sodium bicarbonate, pH 8.3; sodium chloride: Sigma-Aldrich, cat ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were resuspended in 35 mL binding buffer (1X PBS, 1 mM EDTA, 1 mM DTT, Protease inhibitor) then 250 units of benzonase nuclease (Sigma) were added to the resuspension ...
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bioRxiv - Bioengineering 2022Quote: ... the column was pre-equilibrated with 50 mL binding buffer (50 mM NaH2PO4, 300 mM NaCl, 15 mM imidazole, pH 8.0, all from Sigma-Aldrich). The His-tagged Riboswitch-VLPs bound to the column were subsequently washed with 100 mL washing buffer (50 mM NaH2PO4 ...