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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Hyaluronic acid/hyaluronan (HA) was stained overnight using biotinylated HA binding protein (Millipore, Burlington, MA; 385911). All sections were washed and incubated with VECTASTAIN® ABC-HRP kit (Vector laboratories ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... nonspecific binding was achieved by using 0.3 M glycine (Sigma-Aldrich®, St. Louis, MO, USA) in 5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... plates were washed in PBS-Tw and non-specific binding blocked with 3% BSA (Sigma-Aldrich) in PBS for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Unspecific binding sites were blocked by incubation with 3 % goat serum (Sigma-Aldrich, cat.no. G9023-10ML) in PBS at RT for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Non-specific binding was blocked with 300 µL of 5% goat serum (EMD Millipore, Burlington MA) for 1 hour and then incubated with a 1:250 dilution of monoclonal rabbit F4/80 or 1:150 of rabbit Ly-6G (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Biophysics 2024Quote: ... one for binding condition where we added 5 mM ATP (disodium salt, Sigma Product number: A3377) and the other L - buffer was not altered for control condition ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 105-2×106 Ter119+ erythroid cells lysed in TRI reagent (Sigma-Aldrich: T9424) using a Direct-zol RNA MiniPrep kit (Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μg of extracted RNA was used to generate cDNA using ReadyScript cDNA synthesis mix (Sigma; RDRT-100RXN) and the obtained cDNA were diluted 1:3 times with nuclease-free water for performing further experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich, Cat #F1804) or 8 μg anti-U2AF2 antibody (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-RNA polymerase II clone CTD4H8 (Rpb1) antibody was purchased from EMD Millipore (Temecula, CA, USA). Anti-Schizosaccharomyces pombe histone H2B (ab188271 ...
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bioRxiv - Molecular Biology 2022Quote: R loops were stained with an S9.6 antibody that recognizes DNA-RNA hybrid (MABE1095, Sigma-Aldrich) by using the same staining and confocal images procedure as RAD-51 antibody staining ...
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bioRxiv - Plant Biology 2023Quote: ... Fragmented RNA was incubated with 5 mg of anti-N6-methyladenosine antibody (Sigma-Aldrich, Cat. # MABE1006), 5 μl of RNase inhibitor (Lucigen ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2021Quote: Paddle motif at a concentration of 300 μM was prepared with and without 5-doxyl stearate (Sigma-Aldrich, St. Louis, MO) or 16-doxyl stearate (SigmaAldrich ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Genomics 2019Quote: ... cells were incubated for 2 hours in primary antibody (Nestin: Millipore, MAB353, Oct4: Santa Cruz sc-8629), washed in 0.1% PBS/Tween and then incubated for 2 hours in secondary antibody (Goat anti-mouse 488 Life Technologies ab150113 ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Pulldowns were performed using 2 µL of monoclonal anti-FLAG M2 antibodies produced in mice (F3165, Sigma) per reaction and 50 µL of magnetic protein G-coated Dynabeads according to the supplier’s instructions (Dynabeads Protein G for Immunoprecipitation ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution; Millipore). Detection was performed using the SuperSignal West Femto Detection Kit for anti-GFP ...
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bioRxiv - Cell Biology 2021Quote: ... MTs were labeled with rat monoclonal antibody YL 1/2 directed against tyrosinated -tubulin (1:1000, Millipore), or rabbit polyclonal antibody against detyrosinated -tubulin (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation for 2 hours at room temperature with primary antibody (SOX2 [1:200, EMD Millipore AB5603] ...
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bioRxiv - Immunology 2020Quote: ... and the supernatant was taken for immunoprecipitation with 2 μg of anti-ubiquitin antibody (FK2, Merck-Millipore) for 12 hours at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... then incubated for 2 hours with secondary antibody - horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma, UK) diluted 1:1,500 with PBS ...
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bioRxiv - Biochemistry 2020Quote: ... incubated for 2 hours at RT with primary monoclonal mouse anti-FLAG M2 antibody (F1804, Sigma Aldrich) to track migration of 3 x FLAG-tagged eIF2B complex ...
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bioRxiv - Neuroscience 2022Quote: ... All antibodies were diluted in 2% normal goat serum and 0.5% Triton-X (No: T9284, Sigma-Aldrich) in 0.01M PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Following collection of the lysates and isolation of the crude synaptosomal fraction anti-Magi-2 antibody (Sigma) was added to half of the lysate and incubated at 4° C overnight with end over ending rocking ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were incubated in antibody blocking solution with a solution containing 2% fish gelatin (Sigma Aldrich #7765), 5% donkey serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... The DNA-binding domain of human Timeless (DBD; residues 816-954) was cloned into pRSF-Duet1 (Novagen) with an N-terminal His14-SUMO tag and expressed in E ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding was blocked with PBS containing 1% bovine serum albumin (BSA; Sigma, St. Louis, MO, USA) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... incorporating an AAG GAG ATA TAC AT ribosome binding site (RBS) sequence derived from pET-11a (Novagen), and cloned it into the EcoRI and HindIII sites of plasmid pBAD18 (46 ...
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bioRxiv - Biochemistry 2022Quote: ... Binding Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 1% SDS[Sigma-Aldrich]), 2 M Hydroxylamine ...
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bioRxiv - Biochemistry 2022Quote: ... Binding Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 1% SDS[Sigma-Aldrich]), 2 M Hydroxylamine ...
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An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... Non-specific binding sites were blocked by incubation with 3% (v/v) normal donkey serum (Sigma #D9663) in 1X Tris buffered saline (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was filtered through a 0.2 µm low-protein binding syringe filter (Millex Durapore; EMD Millipore) and applied to glutathione Sepharose 4b resin (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... and binding detected using 1 in 25,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was incubated with a mono ADP-ribose binding reagent (MABE 1076, Millipore Sigma, 1:2500) and next treated with a secondary antibody (goat anti-rabbit conjugated to HRP ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 0.05% and non-specific binding sites were blocked with a permeabilization/blocking solution containing PBS (Sigma-Aldrich), 0.5% BSA (Roche ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... PLG binding was revealed by adding 1.5 mM of the chromogenic substrate ortho-phenylene-diamine (OPD) (Sigma) diluted in substrate buffer (25 mM citric acid ...
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Immunology 2023Quote: ... Medium was collected after this period and filtered through 0.45μm ultra-low protein binding filter (Merck Millipore) to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected and transferred to 0.22 μm hydrophilic low protein binding 96-well filter plates (Millipore) and centrifuged at 4000 rpm to remove residual cells ...
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bioRxiv - Microbiology 2023Quote: ... 5% glycerol and 20 mM imidazole (binding buffer) in the presence of protease inhibitor cocktail (Sigma-Aldrich). Cells were lysed using an Emulsiflex B15 high pressure disruptor (Avestin ...
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bioRxiv - Immunology 2023Quote: ... the binding control anti-HLA class I (clone W6/32, 10 mg/ml Sigma, St. Louis, MO), an anti-human MHC class II (HLA DR ...