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Citations for Millipore Sigma :
701 - 750 of 946 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Intestinal permeability was assessed by the fluorescein isothiocyanate conjugated dextran (FITC-dextran) tracer (4 kDa, Sigma-Aldrich, Cat# 68059). Mice were fasted overnight and then administered with 0.5 ml of FITC-dextran (0.5 mg·g-1 body weight ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed three times with warm PBS and incubated with fluorescein diacetate (8 μg/mL, Sigma-Aldrich) and propidium iodide (20 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The permeability was measured exploiting the bovine serum albumin-fluorescein isothiocyanate conjugate (FITC-BSA) (Sigma, Saint Louis, Missouri, USA) [36] ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated overnight in 10 μg/mL wheat germ agglutinin-fluorescein isothiocyanate (WGA-FITC; Sigma-Aldrich, Milan, Italy) to label the fungal wall ...
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bioRxiv - Neuroscience 2023Quote: ... and then received 0.1mL of either 1.5-3% Fluorescein isothiocyanate–dextran (FITC) or Texas Red dextran (70kDa, Sigma-Aldrich #46945 and Thermofisher D1830 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized and administered fluorescein isothiocyanate (FITC)–dextran (0.1 ml, 2 M MW, 5% w/v, Sigma-Aldrich) via tail veil to label blood vessels ...
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bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
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bioRxiv - Microbiology 2022Quote: ... Cellular cortical actin and nuclei were labeled for 30 min with fluorescein isothiocyanate (FITC)-phalloidin (2 μg/ml) (Sigma) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Bioengineering 2022Quote: ... PLL labeled with fluorescein 5-isothiocyanate (PLLFITC) and 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (Rhod) were purchased from Sigma.
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... the transcription reaction was assembled as specified with the addition of 0.5 μl fluorescein-labelled-UTP Solution (Sigma-Aldrich). The reaction was incubated at 37°C for 4 h and the RNA products were annealed by heating the reaction to 75°C for 5 min and subsequently left to cool at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Mice were orally infused with 0.2 ml 40 mg ml−1 4 kDa fluorescein isothiocyanate-dextran (Sigma-Aldrich, USA) 4 hours before sampling ...
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bioRxiv - Physiology 2024Quote: ... the right jugular vein was cannulated for intravenous infusion of physiological saline (100 mM NaCl, 15 mM NaHCO3, 5 mM KCl, 0.25% fluorescein isothiocyanate (FITC)-inulin (Sigma), 2% bovine serum albumin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... we administered 500 mg/kg of 4 kDa fluorescein isothiocyanate– dextran (FITC-Dextran; Sigma-Aldrich, St. Louis, MO, USA) per os to mice after a 4-hour fast ...
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bioRxiv - Cell Biology 2021Quote: ... Rango nuclear import assays were carried out with or without 2.5 mM ATP (pH 7.5, Sigma-Aldrich) and HEK whole cell lysate (2.5 mg/mL in TRB) ...
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bioRxiv - Neuroscience 2021Quote: ... Mitochondria-derived ATP was measured after treatment with 300 mM iodoacetic acid (IAA, Sigma-Aldrich, MO, USA). IAA was added to half of the wells and the cells were then incubated at 37 °C and 5% CO2 for 60 min ...
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bioRxiv - Plant Biology 2024Quote: ... then added to the reaction along with 18mM adenosine 5’-triphosphate (ATP) magnesium salt (A9187; Sigma Aldrich) for plus ATP samples ...
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bioRxiv - Cell Biology 2024Quote: ADP/ATP ratio was determined in cell seeded into poly-D lysine (5 µg/mL, Sigma, P6407) pre-coated 96-Well Flat-Bottom White Microplates (Nunc ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mini-tablet of EDTA-free protease inhibitor) in the presence or absence of ATP (Sigma-Aldrich). The enriched CoA-agarose was washed three times in the buffer and ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mM Tris-HCl (pH7.4)] with ATP detection system containing 5 mM α-ketoglutarate (Sigma-Aldrich 75892), 0.01 mM Ap5A (Sigma D6392) ...
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bioRxiv - Cell Biology 2023Quote: ... for 15 minutes at 37°C in the presence of 10 nm ATP (A1852-1VL, Sigma Aldrich). The mixture was denatured using LDS sample buffer (NP0007 ...
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bioRxiv - Biophysics 2023Quote: ... and relaxing and activating solutions made with 5.95 mM deoxy-ATP (a.k.a. dATP, Millipore Sigma, Burlington, MA) instead of ATP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and compound were preincubated for 5 mins at room temperature before addition of 10□M ATP(Sigma) in 100mM Hepes pH7.4 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... and the apical chamber loaded with 0.5 mL of 300 μg/mL (CA) of sterile sodium fluorescein (NaFlu) (Sigma F6377) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cell Biology 2020Quote: ... mice were stopped serving food for 4 hours and then gavaged with fluorescein isothiocyanate (FITC)-dextran (Sigma, Cat #: F-7250) as previously reported [6].
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bioRxiv - Developmental Biology 2022Quote: Mouse was orally administrated with 15 mg fluorescein isothiocyanate isomer-dextran 4 kDa (FD4, Sigma-Aldrich, St. Louis, MO, USA), serum was collected 4 hours later ...
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bioRxiv - Neuroscience 2022Quote: ... and then administrated 14 ml/kg body weight of phosphate-buffered saline (pH 7.4) containing 22 mg/ml fluorescein isothiocyanate conjugated dextran (FITC-dextran, molecular mass 4.4 kDa; Sigma Chemical, St.) by gavage ...
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bioRxiv - Molecular Biology 2021Quote: ... Poly(deoxyinosinic-deoxycytidylic) acid sodium salt P4929 and Fluorescein-12-UTP RNA Labelling Mix 11685619910 was obtained from Sigma Aldrich, USA ...
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bioRxiv - Molecular Biology 2020Quote: ... and then modified with 1:1 volume ratio with 1 mg/ml biotin-5-fluorescein conjugate (Sigma-Aldrich, cat #53608) followed by three washes with PBS ...
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bioRxiv - Plant Biology 2020Quote: ... followed by incubation in a 1:200 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 0.25μM testosterone 3-(O-carboxymethyl) oxime:BSA-fluorescein isothiocyanate (T-BSA-FITC) conjugate (T5771, Sigma-Aldrich, Munich, Germany) diluted in Tris-Buffer (pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... fumigatus was grown for 5 days at 37 °C and conidia were harvested and labeled fluorescein isothiocyanate (FITC; Sigma-Aldrich) or calcofluor white (CFW ...
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bioRxiv - Genetics 2022Quote: ... Cell death analysis was performed using the ApopTag® Fluorescein in situ Apoptosis Detection Kit (Sigma-Aldrich, Cat. No. S7110) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... FaDu and HDF cells were incubated with different concentrations of fluorescein isothiocyanate (FITC)-labelled lectin from Lycopersicon esculentum (Sigma L0401) for 30 min at 37C ...
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bioRxiv - Microbiology 2022Quote: Mice were fasted for 4hrs before oral gavage with 200uL of fluorescein isothiocyanate (FITC)-dextran (3-5 kDa, Sigma-Aldrich) dissolved in sterile PBS (60mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... Hybridized probes were detected using alkaline phosphatase-conjugated antibodies (Anti-Digoxigenin-AP, #11093274910, and Anti-Fluorescein-AP, #11426338910, Sigma-Aldrich) and visualized by staining with 4-nitro blue tetrazolium (NBT ...
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bioRxiv - Bioengineering 2021Quote: Viability of hMSCs in the photocrosslinked 3D printed constructs was investigated using Live/Dead staining comprised of fluorescein diacetate (FDA, Sigma) and ethidium bromide (EB ...
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bioRxiv - Bioengineering 2021Quote: ... particles were first labeled with AlexaFluor™ 568 streptavidin and suspended in PBS containing 2 mg/mL fluorescein isothiocyanate-dextran (500,000 MW, Sigma). For 35 and 55 µm nanovials the same procedure was used except the nanovials were first diluted 10-fold to reduce fraction of aggregates formed.
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized and the carotid artery was cannulated for the application of fluorescein isothiocyanate (FITC)–dextran (Sigma-Aldrich, 150kD) as described elsewhere (57) ...
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bioRxiv - Cell Biology 2020Quote: ... cell-surface mannoproteins were stained by 1 mg/mL fluorescein isothiocyanate (FITC)-conjugated concanavalin A (Sigma-Aldrich, St. Louis, MO) in P buffer (10 mM sodium phosphate and 150 mM NaCl [pH 7.2] ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the fixed cells were incubated in the staining solution [0.2 mM fluorescein di- β -D-galactopyranoside (FDG, Sigma-Aldrich F2756), 0.2 M citric acid ...
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bioRxiv - Physiology 2020Quote: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Fluorescein In Situ Apoptosis Detection Kit; Millipore) as previously described (Lambert et al. ...
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bioRxiv - Immunology 2021Quote: ... 1×106 yeast cells were incubated in staining buffer (phosphate buffered saline + 0.5% BSA + 2mM EDTA) containing anti-Flag fluorescein isothiocyanate antibody (2 μg/mL; clone M2, Sigma-Aldrich) and the probes for 30 minutes at RT in darkness prior to washing 2 times in ice cold staining buffer ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then incubated for 30 min in EMM5S medium containing 20 μM fluorescein (Sigma-Aldrich 46960-25G-F) or fluorescein-dextran 3,000 MW (ThermoFisher D3305) ...
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... a fluorescein (FITC) labelled 4.4 kDa dextran fraction (negative overall charge conferred to dextran by fluorophore charge) (Sigma-Alrich, FD4) or a FITC labelled 4.4 kDa dextran fraction modified with diethylaminoethyl (DEAE ...
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bioRxiv - Cell Biology 2021Quote: ... A DNA probe against the NST1 region within the rDNA was synthesized using the Fluorescein-High Prime kit (Sigma-Aldrich). The fluorescein-labelled probe hybridization was carried out overnight at 68 °C ...
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bioRxiv - Bioengineering 2022Quote: ... CUBE filled with 1.5% agarose was placed in the gradient chip with 10 μM 40kDa fluorescein isothiocyanate (FITC)-dextran (Sigma, FD40S) in DPBS at one end of the CUBE ...
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...
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bioRxiv - Plant Biology 2022Quote: ... followed by incubation in a 1:10000 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The parasites were supplemented with 10µM DNA-cholesterol nanoparticles and 5mg/mL fluorescein isothiocyanate (FITC)-dextran with a mean molecular mass of 10kDa (Sigma-Aldrich) as a fluid phase marker ...