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Citations for Millipore Sigma :
651 - 700 of 946 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 6dpf zebrafish were gavaged with 1 or 10mM of ATP (Millipore Sigma, St. Louis, MO), dissolved in embryo media ...
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bioRxiv - Cell Biology 2021Quote: ... Equal volumes of cell ghosts and reactivation buffer containing 1 mM ATP (A6559; Sigma-Aldrich) were mixed to induce ATP-dependent actomyosin ring contraction ...
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bioRxiv - Molecular Biology 2022Quote: ... S1C lane 1 where buffer lacked ATP and contained 0.02 units of apyrase (Sigma A6535). Reaction mixtures were incubated for 10 min at 30°C before performing the crosslinking at 4°C for 30 min using long-wave UV light (365nm) ...
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bioRxiv - Biochemistry 2024Quote: ... in PBS with 10 mM MgCl2 and either 1 mM ATP (Sigma Aldrich, A1852-1VL) or 1 mM ATP-γ-thiophosphate (Sigma Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... 0.1 mM ATP and 1 mM EGTA) supplemented with Complete EDTA free protease inhibitor (Sigma) and incubated on ice for 15 min ...
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bioRxiv - Biophysics 2023Quote: ATP stocks were prepared by dissolving adenosine 5’-triphosphate sodium salt (Sigma, St. Louis, MO) in Milli-Q water and adjusting the pH to 7.0 using NaOH and concentrations were determined spectrophotometrically using ε260 = 1.54 × 104 M−1cm−1 [70].
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bioRxiv - Biophysics 2022Quote: ... 100 μl of a solution containing ATP (Adenosine 5’-triphosphate disodium salt hydrate, A2383, Sigma), 3-PG (D-(−)-3-Phosphoglyceric acid disodium salt ...
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bioRxiv - Biochemistry 2022Quote: ... The reaction was depleted of ATP by incubating with 2 U apyrase (Sigma-Aldrich A6410) per 100 μl reaction at room temperature for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... we carried out luciferin-luciferase reactions with an ADP/ATP ratio assay kit74 (Sigma-Aldrich). To release the bound nucleotide from TRiC for measurement ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial ATP synthesis was inhibited with the antibiotic oligomycin (Sigma Aldrich, Catalog No. 75351- 5MG) which was stored at -20°C at a stock concentration of 1 mM (5 mg lyophilized powder in 31.6 mL dH2O) ...
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bioRxiv - Biochemistry 2024Quote: ... To prepare Thorase filaments 1.0 µl of fresh 0.1 M ATP-γ-S (Millipore Sigma) dissolved in running buffer was added to 20 µl of protein having concentration of 0.15 mg/ml for Negative staining EM and 1.20 mg/ml for cryo-EM ...
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bioRxiv - Biochemistry 2024Quote: The ATP hydrolysis activity was determined by using the ADP Colorimetric Assay Kit (Millipore Sigma) following manufacturer’s instruction with some modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... 2µL of a (4mg/mL) solution of the ATP synthase inhibitor oligomycin (Sigma Aldrich, Germany) was added to determine the oligomycin-insensitive reduction in oxygen consumption (leak state) ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were stained with Fluorescein isothiocyanate (FITC)-conjugated cholera toxin B subunit (CTX, 1:1000, Sigma-Aldrich) at 1 μg mL−1 in flow buffer for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The TUNEL protocol was performed using a commercial kit (ApopTag® fluorescein in situ apoptosis detection kit, Millipore). Apoptotic cells were detected as localized bright green cells in a blue background (DAPI ...
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were washed with PBS and incubated in goat anti-rabbit Fluorescein-conjugated secondary antibody (1:500, Millipore) for 2 hours at 25°C ...
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bioRxiv - Neuroscience 2021Quote: ... Approximately 1.5 nl of solution containing 2mg/ml of fluorescein isothiocyanate-dextran 2000kDa (FITC-Dextran 2000, Sigma 52471) was injected into the sinus venosa of anesthetized embryos (Schmitt et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescein isothiocyanate (FITC)-dextran 70 kDa (FTICD) (1 mg/25 g mouse, 0.5% solution in saline, Sigma-Aldrich) was injected into the tail vein and allowed to circulate for 2 min ...
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bioRxiv - Plant Biology 2021Quote: ... aralioides SDX protoplasts were stained with a working concentration of 0.008 µg/µL fluorescein diacetate (Sigma-Aldrich, USA). The samples were analyzed and sorted by a Beckman Coulter MoFlo XDP (Beckman ...
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bioRxiv - Bioengineering 2022Quote: ... the σ2 of the neutral form of the chromophore was also measured versus fluorescein (Millipore Sigma, Darmstadt, Germany) in 10 mM NaOH (pH 12 ...
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bioRxiv - Cell Biology 2020Quote: TUNEL staining was carried out by using an ApopTag® Fluorescein in Situ Apoptosis Detection Kit (S7110, Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were incubated in either Fluorescein Isothiocyanate (FITC)-conjugated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L2895-1MG ...
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bioRxiv - Molecular Biology 2021Quote: Detection of apoptotic cells was determined by using ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Sigma Aldrich). Paraffin sections were deparaffinized and hydrated as described and then treated with proteinase K (20 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... The amplification stage was done using 1 µg ml-1 FITC-tyramide (fluorescein-5-isothiocyanate –tyramide; Sigma-Aldrich) solution for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: Barrier integrity of 3D cultures were assessed using Fluorescein Isothiocyanate (FITC)-Dextran (MW 4,000, Sigma-Aldrich, United Kingdom). FITC-dextran solution (1mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... medium and culture conditions were replaced by 1mg/ml fluorescein isothiocyanate-labelled dextran of 4kDa (FITC-d4; Sigma) at the luminal site and PBS at the basal site ...
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bioRxiv - Plant Biology 2023Quote: Seed mucilage density was evaluated by using Fluorescein isothiocyanate (FITC)-dextran staining [FITC-Dextran 70 (Sigma-Aldrich; 46945) (Voiniciuc et al. ...
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bioRxiv - Neuroscience 2024Quote: Transport studies were performed by measuring the 4/70 kDa fluorescein isothiocyanate–dextran (FD4 - #46944, FD70, Sigma-Aldrich) permeability on cells at 37 °C for 2-72 h ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM Dextrose) containing 0.5% 70 kDa fluorescein isothiocyanate-dextran (FITC-Dextran; Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... These were incubated on ice for 5 min before reactions were initiated by addition ATP (Sigma) to a final concentration of 5 mM (1 µl ATP added to 12 µl protein DNA mix) ...
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bioRxiv - Cell Biology 2021Quote: ... 6 mM ATP or 6 mM ADP (conjugated with sodium salt; Sigma-Aldrich, St. Louis MO) were included along with the contents described above ...
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bioRxiv - Biophysics 2022Quote: ... The imaging buffer was supplemented with an ATP-regeneration system [60 mg/mL creatine phosphokinase (Sigma) and 20 mM phosphocreatine (Sigma)] ...
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bioRxiv - Genetics 2021Quote: ... for 4 hours at 37°C and subsequently treated with 5mM adenosine triphosphate (ATP) (Sigma; A2383) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... ATP in the supernatant was quantified with the “ADP Assay Kit” (MAK133, Sigma-Aldrich, Missouri, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 uM nucleotides (either UltraPure ATP (from the ADP-Glo kit) or AMP (Sigma, Cat#A1752)) were added ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL) in the presence of cMyc-tagged CK2α fusion protein (1 μM) ...
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bioRxiv - Biophysics 2024Quote: ... one for binding condition where we added 5 mM ATP (disodium salt, Sigma Product number: A3377) and the other L - buffer was not altered for control condition ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of MgtAS was incubated with 0.5 µL of 100 mM ATP (Sigma, Catalog # A2383) (for 2 min) ...
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bioRxiv - Microbiology 2023Quote: ... containing Queen7 μM* and a known concentration of ATP (Adenosine 5’-triphosphate magnesium salt, Sigma-Aldrich), was measured at 405 and 488 nm excitation and 528 nm emission wavelengths ...
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bioRxiv - Physiology 2024Quote: ... in the following concentrations: 100 mM adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich A2383), 20 mM L-Glutamate (Sigma-Aldrich G1251) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM ATP and 1% (v/v) pyruvate kinase/lactic dehydrogenase enzyme mix (Sigma-Aldrich P0294) in 1X buffer (50mM K·HEPES pH 7.4 at 25°C ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with a mixture of 8 isotope-labeled chemicals at 1 μM (ATP (Sigma, Cat# 710695), AMP (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Paracellular permeability was assessed by the addition of 0.5 mg/ml fluorescein isothiocyanate-conjugated 70-kDa dextran (dextran FITC, Sigma) to the upper chamber and incubated at 37°C for 30 mins ...
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 0,1 M sodium carbonate buffer (pH 9) and incubated with 0,1 mg/mL of fluorescein isothiocyanate (FITC, Sigma Aldrich) for 1h in the dark with shaking at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed according to manufacturer’s instructions using ApopTag Fluorescein In Situ Apoptosis Detection Kit (Millipore).
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bioRxiv - Biophysics 2021Quote: Assembled actin filaments were stabilized and fluorescently labeled via the addition of 4 µM fluorescein-isothiocyanate phalloidin (Sigma Aldrich). Following a 10-minute incubation ...
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bioRxiv - Bioengineering 2020Quote: ... NPs were synthesized by utilizing copolymers fluorescently-labeled by the conjugation of fluorescein isothiocyanate (FITC, green fluorescence, Sigma-Aldrich) or rhodamine isothiocyanate (RITC ...
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bioRxiv - Microbiology 2021Quote: ... YFP-dsRNA was labeled using the fluorescein RNA Labeling Mix Kit following the manufacturer’s instructions (Sigma, St. Louis, MO). For confocal microscopy examination of fluorescent dsRNA uptake by fungal mycelium ...
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bioRxiv - Cancer Biology 2021Quote: ... TUNEL Assay detecting apoptotic cell death were performed with the ApopTag Fluorescein in situ apoptosis detection kit (S7110, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... DsRed in vascular mural cells) were injected intravenously with 2.5% fluorescein isothiocyanate-dextran (70 kDa FITC-dextran; Sigma-Aldrich), allowing for visualisation of the vasculature ...