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Citations for Millipore Sigma :
7201 - 7250 of 10000+ citations for Anti PIGR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... DivK protein separated based on its phosphorylation state was transferred to a PVDF membrane and detected using a chemiluminescent substrate (SuperSignal West PICO PLUS, Thermo Pierce) after incubation with mouse anti-Flag (1:10000) primary antibody (Sigma, F1804) and a polyclonal goat anti-mouse HRP (1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitations of 3XF-PPIA and 3XF-Mutant PPIA transiently transfected cells were performed with a mouse monoclonal anti-FLAG antibody (clone M2, Millipore Sigma) in 293T cells ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Neuroscience 2021Quote: ... After three washes in PBS sections were incubated with secondary antibodies (anti-Rabbit IgG CF™ 568, 1:1000, Sigma Aldrich, SAB4600084 ...
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bioRxiv - Bioengineering 2020Quote: ... the primary antibody that was used was mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend (EMD Millipore, MAB8251, 1:250). Images were taken at 10x magnification using a Zeiss Axio Observer and Axiocam 503 mono camera (Zeiss).
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with a goat anti-choline acetyltransferase antibody (ChAT; 1:1000; Millipore Cat# AB144P, RRID:AB_2079751) in Tris-Triton buffer containing 2% donkey serum and 0.2% Triton X100 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with a 1:2000 dilution of anti-phospho-Histone H2A.X (Ser139) clone JBW301 antibody (Millipore, 05-636-1) for an hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A594-conjugated secondary donkey anti-goat antibody (1:500, EMD Millipore). Sections were then washed in PBS and blocked with 10% normal goat serum (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Biochemistry 2021Quote: Anti-FLAG immunoprecipitation was performed using 20 μL of packed agarose-conjugated mouse anti-FLAG antibody (M2 Affinity gel, A2220 Sigma-Aldrich) and were targeted either against the exogenously expressed transgenes (HMR+ ...
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bioRxiv - Biochemistry 2021Quote: ... mouse APC-conjugated antibody directed against dsRNA J2 (IF 1:200, Scicons, 10010500) mouse anti-3xFlag M2 (WB 1:25000, Sigma, F1804), rabbit anti-Tubulin (WB 1:4000 ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was washed with TBS-T and incubated for 90 min with anti-rabbit IgG antibody conjugated to alkaline phosphatase (Sigma Alrich), diluted 1:5.000 in TBS-T solution containing 0.75 % BSA ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed in DPBS (5 minutes each wash) and incubated with 2.5 µg/ml an FITC-labeled anti-HA antibody (Sigma, Product # H7411) diluted with 5% BSA in DPBS for 1.5 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich). Catalytically inactive Mt2 (Mt2 C78A ...
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bioRxiv - Plant Biology 2020Quote: ... the FLAG-tagged proteins were immunoprecipitated by incubation of the extracts with 5 μL of anti-FLAG antibody agarose beads (Sigma-Aldrich) for 1 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2020Quote: ... Remaining cell lysate was incubated with pre-washed (three times in 1 ml lysis buffer) 20 μl of antibody-conjugated beads/resin (FLAG: EZview Red ANTI-FLAG M2 Affinity Gel, Sigma-Aldrich; HA ...
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bioRxiv - Plant Biology 2020Quote: ChIP was carried out on four-day-old dark-grown PIF1-OX and wild type seedlings on MS medium using an anti-MYC polyclonal antibody (Sigma-Aldrich) as described (Pfeiffer et al. ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with 1:10,000 vol vol−1 secondary antibody anti-mouse horseradish peroxidase (HRP) conjugate (A5278, Sigma–Aldrich, USA) in 2% weight vol−1 BSA-TTBS ...
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bioRxiv - Microbiology 2020Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) (Fig 4A) ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight with primary antibody against tyrosine hydroxylase diluted in 2% NDS in PBS/TX (1:1000 - rabbit anti-TH, Millipore AB152). The sections were rinsed with PBS (3×10min ...
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bioRxiv - Neuroscience 2020Quote: Cell type specific imaging using fluorescent markers: dopaminergic neurons were specifically labelled using the anti-Tyrosine Hydroxylase (TH) antibody (1:250, rabbit polyclonal, AB152, Sigma Aldrich). This was done on 50 μm thick coronal sections in the midbrain area specifically for the neurons in the ventral tegmental area (VTA ...
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bioRxiv - Neuroscience 2020Quote: ... Position in the retina was determined by staining with a primary antibody sensitive to S-opsin (rabbit-anti-s-opsin, 1:500, Millipore ABN1660) (Sondereker et al. ...
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... in Triton PBS containing 1% v/v NDS and a mixture of between two and four of the following primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500 dilution; AB144P, Millipore, RRID:AB_2079751); goat anti-forkhead box protein P2 (FoxP2 ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Neuroscience 2019Quote: ... brain slices were blocked in PBS-BTA (4% BSA+ 0.25% TritonX100+ 0.02% sodium azide prepared in 1XPBS) for 1 hr followed by incubation with primary antibody-anti-Parvalbumin (1:1000, P3088 Sigma-Aldrich) at RT for 4 hrs with gentle shaking ...
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bioRxiv - Physiology 2021Quote: ... Sections were subsequently incubated with the following primary antibodies for 1 h at room temperature: polyclonal rabbit anti-laminin IgG (L9393, 1:700; Sigma-Aldrich), monoclonal mouse anti-MHCI IgG2b (BA-F8 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were washed and incubated for 1 h at room temperature with primary antibody (anti-HA, mouse, 1:500, Sigma-Aldrich) diluted in PBS containing 1 % bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of the dCasRX-YTHDC1 fusion protein was confirmed by transfection of the plasmid followed by Western Blot with anti-FLAG M2 mouse monoclonal antibody (F1804, Sigma-Aldrich) and anti-YTHDC1 (14392-1-AP ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... and immunoblotting was performed using the following antibodies: anti-K63 ubiquitin (1:4,000; EMD Millipore, cat. no. 05-1308, clone apu3), anti-actin (1:6,000 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... TA muscles were carefully removed and then frozen in liquid nitrogen and stored (−80 °C) for immunoblotting analysis using a mouse IgG2a monoclonal anti-puromycin antibody (clone 12D10, 1:2500; Millipore # MABE343).
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...