Labshake search
Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... and incubated with a Duolink® Anti-mouse plus PLA probe (Sigma-Aldrich) together with a Duolink® Anti-rabbit minus PLA probe (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... horseradish peroxidase (HRP)-conjugated goat α-mouse (Sigma Aldrich A4416; 1:10 000) and HRP-conjugated goat α-rabbit (Sigma Aldrich A0545 ...
-
bioRxiv - Bioengineering 2023Quote: ... anti-Mouse IgG (whole molecule)– Peroxidase antibody produced in goat (A4416; Sigma-Aldrich) in 1:18,000 dilution ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary antibodies included mouse monoclonal anti-NeuN (1:500, Millipore Sigma, CAT# ABN78) and secondary antibodies included goat anti-rabbit 488 (1:500 ...
-
bioRxiv - Immunology 2024Quote: Mouse neutrophils were isolated using Histopaque-1077 and Histopaque-1119 (Sigma-Aldrich, #11191). Cells were then stimulated with Phorbol 12-myristate 13-acetate (PMA ...
-
bioRxiv - Microbiology 2024Quote: ... Plates were coated with goat anti-mouse IgG (1 µg/mL; Sigma-Aldrich) to quantify the concentration of total IgG or RSP (10 μg/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... Primary antibodies we used include mouse monoclonal antibody to dsRNA (Millipore Cat# MABE1134); Rabbit polyclonal to Iba1 (Abcam Cat# ab108539) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Slides were incubated with mouse anti-γH2A.X primary antibody (Sigma-Aldrich, 05-636) at 1:500 in blocking buffer for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used were as follows: anti-SMA (mouse, 1:200, Millipore, CBL171), anti-βIII tubulin (rabbit ...
-
bioRxiv - Neuroscience 2024Quote: ... mouse anti-glial fibrillary acidic protein (GFAP Cy3-conjugated Sigma-Aldrich, C9205, RRID:AB_476889), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba1 ...
-
bioRxiv - Neuroscience 2024Quote: ... and the plasma membrane protein NaK1 (mouse monoclonal, Millipore, 05-369, 1:2000). Membranes were developed using the ECL Prime Western Blotting System (Amersham Protran ...
-
bioRxiv - Neuroscience 2024Quote: ... and mouse anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (1:10,000, MAB374, Sigma-Aldrich). Secondary antibodies used were ...
-
bioRxiv - Cancer Biology 2024Quote: ... Immunocomplexes were detected with horseradish peroxidase-conjugated rabbit or mouse secondary antibodies (Sigma) followed by enhanced chemiluminescence reaction (Millipore ...
-
bioRxiv - Immunology 2024Quote: ... Goat Anti-Mouse IgG (H + L) (#71045, 1:5000; Sigma-Aldrich; RRID: AB_11211441) or VeriBlot Detection Reagent (#ab131366 ...
-
bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: Mouse anti-α-tubulin (1:1000 Sigma T6074); Rabbit anti-α-tubulin (1:2000 Invitrogen PA519489) ...
-
bioRxiv - Immunology 2024Quote: ... followed by crosslinking with 10 µg/ml of anti-mouse IgG (Sigma-Aldrich) at 37°C for the indicated incubation times ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by incubating with the secondary antibodies (anti-Mouse MINUS, DU092004, Sigma-Aldrich; anti-Rabbit PLUS ...
-
bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-INCENP (Millipore, 05-940, 1:250-IF 1:1000-WB) and Rat anti-Tubulin (EMD Millipore ...
-
bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal anti-HCoV-OC43 nucleocapsid protein (1:1000, Millipore Sigma, MAB9012). Secondary antibodies coupled to HRP were purchased from molecular probes and Jackson Immuno-research.
-
bioRxiv - Molecular Biology 2024Quote: ... and then incubated with goat anti-mouse conjugated with HRP (#AP127P, Millipore-Sigma) in 5% milk TBST for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... and then incubated with goat anti-mouse conjugated with HRP (#AP127P, Millipore-Sigma) in 5% milk TBST for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... inactivated Mouse Embryonic Fibroblasts (MEFs) or on gelatin-coated multiwell plates (Sigma, G1890) in mESC cell medium ...
-
bioRxiv - Neuroscience 2024Quote: ... mouse IgG1 anti-Pan Nav 1 (1:100; N419/78; Millipore, MA, USA), mouse IgG1 anti-Nav 1.6 (1:100 ...
-
bioRxiv - Microbiology 2024Quote: ... and probed with 1:5000 dilution of mouse monoclonal α-FLAG antibodies (Sigma) and a 1:12,000 dilution of mouse monoclonal α-RpoA (BioLegend ...
-
bioRxiv - Immunology 2024Quote: ... followed by detection with a goat anti-mouse IgG HRP (1/1000; Millipore) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: mouse anti-FLAG (1:5000, Sigma-Aldrich F1804), rabbit anti-Tor1 (1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... mouse monoclonal anti-Smooth Muscle Actin antibody (Clone 1A4) was from Sigma (A2547).
-
bioRxiv - Cell Biology 2024Quote: ... using the following primary antibodies: mouse anti-RAD2l (0S-908, Merck Millipore, l:S00), rabbit anti-SMC2 (abl04l2 ...
-
bioRxiv - Cell Biology 2024Quote: Mouse testes were harvested and fixed in 10% buffered formalin (Sigma-Aldrich, USA) overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Mouse anti-FLAG M2 affinity gel was obtained from Sigma Aldrich (A2220-1ML). Mouse anti-FLAG M1 was made in-house.
-
bioRxiv - Systems Biology 2024Quote: ... mouse anti-3R Tau monoclonal antibody-RD3 (1:800; Millipore sigma# 05-803); Rabbit anti-4RTau monoclonal antibody (1:1000 ...
-
bioRxiv - Biophysics 2020Quote: ... the following target sequences were used: 5’-GCTTCAGGATTCAATGCCATGG-’3 (#1) using the all-in-one CRISPR/Cas9 plasmid (Sigma-Aldrich) followed by cell-sorting for GFP expression to generate single cell clones with disrupted ANXA4 reading frame.
-
bioRxiv - Developmental Biology 2021Quote: ... Zebrafish lines expressing transgenic Spaca6 were generated by injecting the spaca6 expression construct with Tol2 mRNA into spaca6+/− zebrafish embryos (15 ng/μl of the plasmid in RNase-free water, 35 ng/μl Tol2 mRNA, 0.083% phenol red solution [Sigma-Aldrich]), following standard procedures ...
-
bioRxiv - Cell Biology 2020Quote: HeLa cells were co-transfected with each Rubicon plasmid and mRFP-Rab79 and stained with anti-FLAG M2 antibody (Sigma) at 48h post-transfection.
-
bioRxiv - Cell Biology 2020Quote: For purification of full-length Sar1, we used a previously reported plasmid (McMahon et al., 2012) using the pET21b (Novagen) backbone that expresses full-length Sar1 from Saccharomyces cerevisiae with a C-terminal hexahistidine tag ...
-
Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... one-cell-stage embryos were injected with purified plasmid DNA diluted in ddH2O supplemented with 0.05% phenol red (Sigma-Aldrich). Concentrations of individual constructs ranged from 10 to 25 ng/μL ...
-
bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
-
bioRxiv - Cell Biology 2022Quote: RAW-ASC and iBMDM cells were transfected with custom CRISPR gRNA plasmid DNA (U6-gRNA:CMV-Cas-9-2A-tGFP) (Sigma-Aldrich) using FuGENE HD transfection reagent (Promega) ...
-
bioRxiv - Biochemistry 2022Quote: ... were transformed with the plasmid and plated onto Luria broth (LB) agar supplemented with 50 μg mL−1 kanamycin (Sigma) and grown overnight at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reaction was stopped by the addition of 500 ng of plasmid (size > 10 kb) and 0.1 pmol of ATP-γ-S tetralithium salt (10102342001-Roche, Sigma) followed by incubation for 30 min at 30°C ...
-
DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
-
bioRxiv - Immunology 2019Quote: MCA205 and B16F10 mouse cell lines are transfected with the plasmid YFP-Globin-SL8-intron or with the PCDNA3 empty plasmid (negative control) with the transfection reagent jetPRIME (Ozyme) or GeneJuice (Millipore) respectively according to each manufacturer protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... Xa factor site and StreptagII sequence in its N-terminus are digested by HindIII and BamHI and then inserted into a pET-20b plasmid (Novagen). The over-expression in BL21 (DE3 ...
-
bioRxiv - Biochemistry 2019Quote: ... Xa cleavage site and StrepTagII sequence in its N-terminus part were digested with XbaI and Hind III enzymes and inserted into pET-20b plasmid (Novagen). The over-expression in Rosetta (DE3 ...
-
bioRxiv - Microbiology 2019Quote: ... These plasmids were transfected into Rossetta 2 cells and this system was used for expression according to manufacturer’s instructions (EMD Millipore) for expression ...
-
bioRxiv - Cancer Biology 2019Quote: ... Desired constructs were amplified from the complete RNF114 sequence with primers that contained 20 base pairs of homology to a pET24a plasmid (Novagen) that had been modified to contain a His8-MBP-TEV sequence between the Nde1 and BamH1 restriction sites ...
-
bioRxiv - Developmental Biology 2020Quote: ... The cDNA sequence corresponding to sim-PD (aa 361-672) was PCR amplified and cloned in-frame with a 6xHis-Tag into a modified pET-14b plasmid (Novagen). The expression plasmid was transformed into BL21 competent E ...
-
bioRxiv - Biochemistry 2020Quote: QnrB1/DNA binding competition assays were performed as follows: 90 bp Cy5-labelled oligonucleotide encompassing the strong gyrase binding site from plasmid pBR322 was ordered from Sigma and annealed with an antisense unlabelled oligo by heating to 99°C and gradual cooling ...
-
bioRxiv - Genetics 2021Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
-
bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 cells/ml were co-transfected by the linearised Defbac viral backbone and the plasmids encoding for exocyst subunits or PIP5K1C using ESCORT-IV (Sigma). After 5 days ...