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Citations for Millipore Sigma :
7251 - 7300 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2021Quote: ... 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46, T9450, mouse, 1:1000, Sigma-Aldrich).
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... TA muscles were carefully removed and then frozen in liquid nitrogen and stored (−80 °C) for immunoblotting analysis using a mouse IgG2a monoclonal anti-puromycin antibody (clone 12D10, 1:2500; Millipore # MABE343).
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...
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bioRxiv - Biophysics 2020Quote: ... permeabilized cells were blocked with 3 % BSA in PBS for 30 min at room temperature and then incubated with mouse monoclonal anti-alpha-tubulin antibody (T6199, clone DM1A, Sigma-Aldrich) diluted at 1:200 in 3 % (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibody staining was carried out by placing coverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma-Aldrich, diluted 1:300 in PBS containing 2% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with horseradish peroxidase (HRP)-conjugated goat polyclonal anti-mouse IgG (1:5,000 dilution, EMD Millipore Etobicoke, Ontario, Canada). Supersignal West Femto enhanced chemiluminescence substrate (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Neural dendrites were detected with the neurofilament marker anti-SMI 311 mouse monoclonal antibody (1:600, Millipore Bioscience Research Reagents, USA). The day after ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: The following antibodies and dilutions for Western blot (WB) and immuno-fluorescence (IF) were used in this study: mouse monoclonal anti α-tubulin (clone DM1A, Sigma, T9026 ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-rabbit IgG, 1:10,000, Jackson ImmunoResearch, cat# 111-035-144; 80-25 kDa: goat anti-mouse IgG, 1:4,000, Millipore, 12-349) diluted in TBST for 2 h at 25 °C ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Neuroscience 2020Quote: ... in TBS with 0.4 % Triton X-100 and incubated with a mouse anti-GFAP antibody (1:500, clone G-A-G, Sigma: G 3893) in blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at RT with a combination of two primary antibodies: a mouse monoclonal anti-PCNA (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Microbiology 2021Quote: ... Membrane was then washed 3x for 10 min with TBST and subsequently incubated with the alginate antibody (Sigma, monoclonal, anti-mouse) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Molecular Biology 2022Quote: Blots were washed three times for 15 minutes with PBS-T and probed 45 minutes with secondary antibodies (rabbit anti-mouse (Sigma A4312), 1:10000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Treg cells were stimulated in complete RPMI-1640 culture medium at 37 °C for 5 days with plate-bound 0.2 or 1 μg/mL anti-human CD3 mAb (clone HIT3α) and either plate-bound 30 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich). The culture supernatants were harvested ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were washed with TBST buffer for three times and subsequently incubated in 1:10,000 dilution of secondary antibody (anti-mouse IgG whole molecule conjugated with alkaline phosphatase) (A3562, Sigma-Aldrich, USA). The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Cell Biology 2019Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4 °C with mouse anti-SMI31/32 (to label neurofilament proteins; Millipore NE1022/NE1023; 1:1000) and chicken anti-myelin basic protein (to label myelin ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... and mouse monoclonal anti-DNA/Histona H1 antibody (1:100; catalog number 05-457/clone AE-4; Merck Millipore, MA, EUA). Alternatively ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... β-actin was also detected as a loading control using a 1:10,000 dilution of mouse anti-β-actin (Sigma, A5441). A 1:3,000 dilution of goat anti-mouse (Dako ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Developmental Biology 2020Quote: ... terminus or c.terminus anti-SPAG17 antibodies (previously described by Zhang and colleague, Zhang, Jones et al. 2005) and 1:1000 Mouse anti-Tubulin (Sigma #T6199) antibodies) ...
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bioRxiv - Genetics 2019Quote: ... Membranes were incubated overnight with agitation at 4 °C with 1:2000 monoclonal mouse anti-alpha-tubulin (Sigma-Aldrich; clone DM1A) and 1:1000 monoclonal mouse anti-Cas9 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ChIP experiments were performed in AtT-20 cells as previously described (29) using 5 μg of antibody (anti-FLAG-M2, mouse monoclonal, Sigma F3165). ATACseq was performed as previously described (4) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was removed entirely and cells were suspended in 100 μl of the same TBS solution containing mouse monoclonal ANTI-FLAG M1 antibodies (Sigma, F3040) at a 1:500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
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bioRxiv - Molecular Biology 2020Quote: Antibodies and dilutions for IF and Duolink were the following: mouse monoclonal anti-RPAP3 at 1:250 dilution (Sigma-Aldrich, SAB407956); polyclonal rabbit anti-TRBP at 1:100 dilution (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
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bioRxiv - Neuroscience 2019Quote: ... Robert Froemke and Moses Chao at NYU (1:200) and mouse anti-TH primary antiserum (MAB5986, Millipore, Burlington, MA; 1:2000) for 48 hrs at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: Six-milliliter lysate of BL21 Star (DE3) expressing pET28a-FLAG-dDYNLL1 was incubated with 200µl mouse anti-FLAG magnetic beads (clone M2, Sigma-Aldrich, #M8823) at 4°C for 3 hours ...
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bioRxiv - Microbiology 2019Quote: ... alkaline phosphatase-conjugated (1:10,000) or goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (1:10,000) secondary antibody (Sigma, St. Louis, MO). Immunoreactive bands were detected by the addition of NBT/BCIP (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated overnight at 4°C in PBST-NGS containing mouse monoclonal anti-α-Tubulin-FITC antibody (Sigma-Aldrich) at a dilution of 1:200 ...
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bioRxiv - Genetics 2021Quote: ... was used for staining DNA and mouse anti-a tubulin monoclonal antibody DM1A (1:50) conjugated with FITC (Sigma, St. Louis) was used for staining microtubules ...
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bioRxiv - Physiology 2020Quote: ... we incubated them with anti-rabbit PLUS and anti-mouse MINUS PLA probes (1 hour at 37°C) diluted in Duolink® incubation buffer (1:200; Sigma), followed by three washes with PBS ...