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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... for 10 mins and incubated in blocking PBS solution containing 2% BSA (AppliChem, 9048-46-8) and 0.2% Triton™ X-100 (Sigma-Aldrich, 9002-93-1) for 30 mins to permeabilize cell membrane and block unspecific antigen binding ...
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bioRxiv - Cell Biology 2021Quote: ... and were then freed from any remaining cumulus cells by a 1–2 min treatment of 0.1 % hyaluronidase (Sigma-Aldrich, St. Louis, MO, ISA) dissolved in M2 medium (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1 mM EDTA, 10 mM KCl, 1x Complete® [Roche, 1697498], 1 mM DTT, 1x phosphatase inhibitor cocktails 2 and 3 [Sigma Aldrich, P5726/P0044]). After incubation on ice for 15 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 500 and 1000 μg/mL in 1 mL of extraction solution consisting of propan-2-ol with heptadecane at purity of 99% (Sigma Aldrich, cod. 128503-100G) at concentration of 50 μg/L.
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bioRxiv - Immunology 2022Quote: ... and 50 μM 2-mercaptoethanol (D10 medium) in 12 well plates in the presence of adenosine (0–1 mM) (Sigma, St. Louis, MO, USA); in the presence of each adenosine receptor agonist (0–10 μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... airway mechanics were measured by challenging pigs to increasing concentrations of methacholine hydrochloride (MeCh) (0, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) via an Aeroneb™ nebulizer (Aerogen Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... The EV pellets were resuspended in 1 ml of 20 mM Tris-HCl pH 7.5 and mixed with 2 ml of 60% Optiprep™ (Sigma-Aldrich, St. Louis, MO) to create a 40% layer ...
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bioRxiv - Bioengineering 2022Quote: ... 90°C), incubation time (1 min, 2 min) and permeabilization status (with or without incubation in 0.05% Triton-X [Sigma] in PBS for 10 min) were tested (Figure 2B) ...
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bioRxiv - Biophysics 2022Quote: ... the assembled structure was coated with 100 µg.mL−1 poly-L-lysine (20 kDa) grafted with polyethylene glycol (2 kDa) (PLL-PEG) (Sigma-Aldrich, St Louis, MO, USA) for 30 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old Col-0 and lnccobra1-2 seedlings were crosslinked in PBS with 1% formaldehyde (v/v) (Sigma-Aldrich; St. Louis, MO, USA) added and placed under vacuum for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Lipids were extracted using 4 ml Chloroform (CHCl3)/methanol (CH3OH) (2:1, v/v) and shaken with an orbital shaker (IKA® VX® basic Vibrax®, Sigma-Aldrich) at 1,500 rpm for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The thawing and culturing medium (NB Plus +) consisted of Neurobasal Plus Medium containing 2% B-27 Plus Supplement and 1% penicillin–streptomycin (PS; Sigma−Aldrich, cat. no. P0781). After thawing ...
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bioRxiv - Immunology 2022Quote: ... were cultured in complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies], and 50mM 2-mercapto-ethanol [Sigma-Aldrich]) on plate-bound anti-CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... for 2 h at room temperature and then blocked for 1 h at room temperature with 5 % neonatal calf serum (Millipore Sigma, St. Louis, MO) in PBS ...
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bioRxiv - Bioengineering 2020Quote: HDFs and hMSCs were cultured in their appropriate media compositions supplemented with 1% L-ascorbic acid 2-phosphate (AsAP, Sigma-Aldrich, A8960, the Netherlands), 15% FBS and 1% PS ...
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bioRxiv - Plant Biology 2020Quote: ... frozen leaf samples were ground in liquid nitrogen and incubated in 4 ml lysis buffer (50 mM Tris-HCl pH7.5, 150 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT, 2% PVPP) at 4 °C for 15 min using a rotator to form a homogeneous suspension ...
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bioRxiv - Neuroscience 2020Quote: ... one containing 20% ethanol adulterated with 100 μM (Days 1 and 2) or 250 μM (Day 3) quinine hemisulfate (Sigma-Aldrich, St. Louis, MO) and the other containing water ...
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bioRxiv - Neuroscience 2019Quote: ... for 12 hrs at room temperature and finally incubation with the refractive index matching solution BABB (benzyl alcohol + benzyl benzoate 1:2; Sigma-Aldrich, 24122 and W213802), for at least 24 hrs at room temperature until transparency was achieved ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μM FeSO4) were added in the form of crystals to D-depleted water (≤1 ppm deuterium oxide; Sigma-Aldrich, St. Louis, MO, USA), followed by the carbon source (glucose crystals or 99 % glycerol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 500 and 1000 μg/mL in 1 milliliter of extraction solution consisting to propan-2-ol with heptadecane at purity of 99% (Sigma Aldrich, cod. 128503-100G) at concentration of 50 μg/L.
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bioRxiv - Developmental Biology 2020Quote: ... 10% fetal bovine serum in PBS) for 2 hours in room temperature and stained with anti α-Tubulin (DM1A; Sigma-Aldrich, 1:1000, overnight) followed by secondary antibody staining (Alexa Flour 488 anti-mouse 1:1000,2 hours in room temperature).
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bioRxiv - Neuroscience 2023Quote: ... light was applied for at least 2 seconds during periods of increased IPSC activity induced by a 500 ms puff of acetylcholine (1 μM, Millipore Sigma, St. Louis, MO) through a picospritzer ...
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bioRxiv - Neuroscience 2022Quote: Brain punches were homogenized in radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase inhibitor cocktail 1 and 2 and protease inhibitor cocktail (Sigma Aldrich, Saint Louis, MO) followed by centrifugation at 16,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for at least 20 hours at room temperature with a combination of the following primary antibodies in PBS-TX with 2% NGS or NDS: guinea pig anti-VGLUT1 (1:1000; AB5905, Sigma-Aldrich, St. Louis, USA), guinea pig anti-VGLUT2 (1:20,000 ...
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bioRxiv - Microbiology 2024Quote: ... pylori culture was adjusted to a concentration of 2*108 bacteria/ml in BHI supplemented with 20% FCS and was stained with TAMRA (Sigma, 1 µl TAMRA/ml) for 30 min shaking at 120 rpm in microaerobic conditions at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... the growth medium was changed to a fusion medium (DMEM supplemented with 2% heat inactivated horse serum, insulin [10 μg/ml, Sigma-Aldrich] and 1% antibiotics). Experiments were performed at the myotube stage ...
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bioRxiv - Neuroscience 2024Quote: ... The iL3 was then transferred in a single droplet to a 2% Nematode Growth Media (NGM) agar plate [67] and paralyzed with nicotine (Sigma N3876, 1% in dH2O). The iL3 was then scored for activation based on the presence (activated ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibody were diluted in Blocking Buffer as described below and incubated with samples for 2 hours: γ-H2AX monoclonal mouse antibody JBW301 (Millipore Sigma, 1:1000). Samples were washed with Blocking Buffer twice for 5 minutes each then treated with secondary antibody diluted 1:200 for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by the addition of 28 μl of a stop reaction mixture: 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... des-Arg9-Leu8]-BK (B1R antagonist) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-Bromoguanosine 3′ ...
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bioRxiv - Microbiology 2023Quote: ... To improve DNA recovery and avoid the interference of carbon with the extraction, 25 µL of 20 g·l−1 autoclaved (20 min, 121° Celsius, 2 bar) skimmed milk solution (Sigma Aldrich, Saint Louis, MO, USA) were added to the extraction tube ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of the resulting supernatant was mixed with 115 µL of buffer 2 (1 mM Tris, 150 mM NaCl) both with and without 0.8 mM terbium chloride (Sigma-Aldrich, St. Louis, MI, USA). Following a 15-minute incubation ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were thawed at 37 ºC and incubated in darkness with 4 μg/ml Hoechst (HO33342; 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate, Sigma-Aldrich, St Louis, MO, USA) DNA stain in solution buffer (1 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 1,3(d5)-dipentadecanoyl-2-(9Z-octadecenoyl)-glycerol (15:0/18:1/15:0-d5 TG) were obtained from Sigma Aldrich (St Quentin-Fallavier, France). QReSS kit containing labelled metabolites (stable isotope-labelled molecules was obtained from Cambridge Isotope Laboratories Inc ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The solution in each well was changed every 1-2 days with FSW containing 60□μg/mL penicillin and 50□μg/mL streptomycin (Sigma-Aldrich # P3032 and #S6501).
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bioRxiv - Neuroscience 2024Quote: ... Cortex samples were placed in a 2 mL Dounce homogenizer on ice with 1 mL ice cold Nuclei EZ lysis buffer (Sigma, #EZ PREP NUC-101) and homogenized 15-20 times with pestle A and then 15-20 times with pestle B ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
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bioRxiv - Cell Biology 2020Quote: Anesthetised Cx3cr1GFP/+ and Cx3cr1GFP/GFP animals (n = 5, 6 respectively) were injected intraperitoneally with rhodamine B (Sigma-Aldrich) to label blood vessels ...
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bioRxiv - Molecular Biology 2020Quote: ... here the pH of SM or IMF were adjusted to pH4.6 using 6 N HCl (Sigma-Aldrich, Ireland) to remove caseins ...
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bioRxiv - Molecular Biology 2021Quote: ... Cultures were grown in 6-well plates containing 40 sterile 5 mm glass beads per well (Sigma, 18406). Two replicates were set up ...
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bioRxiv - Immunology 2019Quote: ... 6-mm skin biopsy punch samples were digested for 30 minutes at 37°C using Collagenase VIII (Sigma). At the end of the digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were cut at a 6 μm thickness and stained with Haematoxylin (Electron Miscroscopy Sciences) and Eosin (Sigma). For Alcian blue PAS staining ...
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bioRxiv - Bioengineering 2019Quote: ... or standard 6-well tissue culture plastic dishes were incubated with human plasma fibronectin (Millipore Sigma, Catalog #FC010) at a concentration of 10 μg/ml diluted in PBS (3 ml/well of ECM protein solution added ...
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bioRxiv - Cell Biology 2019Quote: ... 6 × 104 cells grown on glass coverslips for 24 h were labelled with 5 µM SBFI-AM (Sigma) and 0.1 % v/v Pluronic F-127 (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... the remaining mesenchymal fraction was incubated with 6 mg/ml Dispase II/0.05% trypsin solution (Sigma-Aldrich, 04942078001) supplemented with 1 U/ml DNaseI (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/ml hyper interleukin-6 (kindly provided by S. Rose-John) and 0.2% Methylcellulose (Sigma-Aldrich, Germany). Cultures were monitored weekly for sphere growth.
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Systems Biology 2021Quote: ... C57Bl/6 mice were administered phosphate-buffered saline (PBS) or LPS (Escherichia coli O55:B5, Sigma-Aldrich, UK) via i.p ...