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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... They were then differentiated into cardiac mesoderm using RPMI B27-medium (RPMI1640 GlutaMAX+ 2% B27 supplement without insulin, 200 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate [Sigma] and 1% Penicillin/Streptomycin [all ThermoFisher Scientific unless otherwise indicated] ...
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bioRxiv - Cell Biology 2019Quote: ... Central channels were loaded with cells and 2 mg/mL fibrinogen after addition of bovine thrombin (2 U/ml) (Sigma). After polymerization ...
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bioRxiv - Cell Biology 2021Quote: ... and re-suspended in equal volume of 2×Native lysis buffer (200mM Tris pH 7.5, 150mM NaCl, 2mM PMSF, 2×Protease Inhibitor Cocktails (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2021Quote: ... of 2 in the presence of 2.2 mM ferrihydrite (Schwertmann and Fischer, 1973; Stookey, 1970) and 2 mM ferrozine (Sigma-Aldrich). Where indicated ...
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bioRxiv - Neuroscience 2021Quote: ... NEL-MG were treated with 2 μL of red fluorescent latex beads (2 μm, Sigma-Aldrich, St. Louis, MO, USA), HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (2 μL) ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Plant Biology 2022Quote: ... 100 mM ammonium bicarbonate [AB], 10 mM Tris[2-carboxyethyl]phosphine hydrochloride, 50 mM 2-chloroacetamide, 0.2% protease inhibitor [Sigma, P8340]), and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were placed into 2 ml cold EZ lysis buffer in a 2 ml glass dounce tissue grinder (Sigma, D8938) and homogenized by hand 25 times with pestle A followed by 25 times with pestle B while incorporating a 180-degree twist ...
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bioRxiv - Neuroscience 2022Quote: ... mice were injected with the noradrenergic neuron specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4; Sigma, C8417) once at a dose of 50 mg/kg (i.p.) ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: ... acr-2(gf) double mutants were washed into a 2 mL solution of M9 media with 47 mM ethyl methanesulfonate (Sigma) and incubated at 22°C on a spinning wheel for 4 hours ...
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bioRxiv - Immunology 2023Quote: Duodenal biopsies were collected in ice-cold RPMI-1640 and processed into single- cell suspensions by treatment with 2 mM EDTA in 2% (v/v) FBS/PBS followed by digestion with collagenase (Sigma) and cryopreservation as previously described.22 PBMCs were isolated from peripheral blood by density gradient centrifugation followed by cryopreservation ...
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bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was stopped by FBS then cell were washed 2 time with PBS-based staining buffer containing 2% fetal bovine serum (FBS) and 0.01% NaN3 (Sigma-Aldrich), blocked Fc IgG receptors by pre-incubated with CD16/32(93 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown overnight in SC-URA medium with 2% dextrose and were plated in SC-URA plates in the presence of 2% dextrose (Sigma) or 2% galactose (Difco) ...
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bioRxiv - Biochemistry 2022Quote: Phosphate buffer saline solution (Ca 2+, Mg 2+ free) was prepared using Phosphate Buffer Saline (PBS) powder (Sigma Aldrich, USA.). 9.6 grams of the powdered reagent was added to 990 milliliters of ultra-purified milli-Q water (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... (Catalog No: PCT0809, Himedia), 10 mM of 2-Naphthaleneacetic acid (2-NAA, inactive form of auxin) (Catalog No: N4002, Sigma) and 20 nM of synthetic auxin 2,4-Dichlorophenoxyacetic acid (2,4-D ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected with 2 µg of scrambled siRNA (MISSION siRNA Universal Negative Control #2 Sigma-Aldrich SIC002-5x1nmol) or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG ...
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bioRxiv - Biochemistry 2023Quote: ... Standard amino acids and 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) were purchased from Sigma-Aldrich (St Louis, MO). JPH203 ((S)-2-amino-3-(4-((5-amino-2-phenylbenzo [d]oxazol-7-yl)methoxy)-3,5-dichlorophenyl ...
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bioRxiv - Neuroscience 2023Quote: ... pH 9.5) before being transferred to the staining buffer (2% NBT-BCIP stock solution [Sigma-Aldrich, 11681451001] in Buffer 2). Samples were allowed to be stained for 30 min in the dark and were then dehydrated through a series of increasing methanol concentration (20% ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma] ...
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bioRxiv - Genomics 2024Quote: ... the tissue was transferred into a 15 ml conical tube with 2 ml CM and 2 ml extracellular matrix digestion media (ECM) containing collagenase (Sigma), DNase (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX, 2% B27 supplement without insulin, 200 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Retinas were manually dissected with gentle up and down passes in ice-cold Ca+2–Mg+2-free Tyrode’s buffer and treated with papain (P3125, Sigma–Aldrich) 20 min and deoxyribonuclease I (18047-019 ...
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bioRxiv - Cell Biology 2024Quote: ... To assess glycolysis-related ECAR an additional injection of 100 mM 2-deoxy-d-glucose (2-DG, from Sigma-Aldrich), an inhibitor of glycolysis ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were sequentially treated with short (2 s) applications (separated by 2 min BS washout) of 100 μM GABA (Sigma), 100 μM glutamate (together with 30 μM NMDA receptor co-agonist glycine ...
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bioRxiv - Cell Biology 2024Quote: ... Phenotypical reversal of T4-2 cells was performed by adding 10 µM Tyrphostin AG 1478 (Sigma, cat; # 175178-82-2) to the medium on day zero of 3D culture as previously described (Fiore et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The phosphatase assay was carried out using the Ser/Thr Phosphatase Assay Kit 1 (Millipore Sigma (MA, USA)) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Nuclear and cytoplasmic extracts were prepared from differentiated THP-1 cells using Nuclei EZ Prep kit (Sigma-NUC101) both from un-stimulated and MprA stimulated cells ...
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bioRxiv - Genetics 2020Quote: Cells were crosslinked with 1% formaldehyde for 10min before chromatin fragmentation with EZ-enzyme kit 17-375 (Millipore) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of the supernatant was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of each sample was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from 1 million cells in triplicates using GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μM forskolin (Sigma, Cat No. F3917). Medium was changed every day throughout this protocol ...
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bioRxiv - Cell Biology 2020Quote: ... with tunicamycin (2 μg/g Cat: T7765, Sigma) which was prepared by dissolving the compound in a small volume of DMSO (100μL ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM tris(2-carboxyethyl)phosphine hydrochloride (Sigma), 1X cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... 2-phospho-L-ascorbic acid (AA2P – Sigma-Aldrich) 50 μg/μL ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg/ml trypsin (Dutcher Dominique or Sigma), 5 μg/ml insulin (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM cyclooctatetraene (COT, #138924, Millipore Sigma). The buffer was prepared fresh by mixing stock solutions right before imaging and replaced every 90 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µg/mL β-estradiol (Sigma cat # E2758), 50 μg/mL gentamicin sulfate ...
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bioRxiv - Microbiology 2020Quote: ... and 2 mg/ml L-cysteine (Sigma-Aldrich) (Referred to now as SAB+).
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bioRxiv - Immunology 2021Quote: ... 2 mM L-Glutamine (Sigma-Aldrich; Merck KGaA) and 100 μM 2-mercaptoethanol (Sigma-Aldrich ...