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Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred from the gels to Immobilon PVDF membranes (Merck Millipore Ltd.). The membranes were immersed in appropriate blocking buffer (Tris-buffered saline 0.05% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Purified proteins were concentrated using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore) or Microsep™ Advance 3kDa (PALL ...
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bioRxiv - Genetics 2022Quote: ... Protein bands were developed with Immobilon Western Chemiluminescent HRP Substrate solutions (Millipore Sigma #WBKLS0050) and visualized under UV light using BioRad’s ChemiDoc MP™ Imaging Platform ...
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bioRxiv - Developmental Biology 2022Quote: ... The proteins were transferred from polyacrylamide gel onto a polyvinylidene difluoride membrane (Millipore IPVH00010). After the transfer ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were eluted from pooled beads with 0.5mg/ml FLAG peptide (#F3290, Millipore Sigma) by shaking at 1000 rpm for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... and immunoprecipitated proteins were eluted 3 times with 150µL 3x-FLAG peptides (Sigma, F4799) for 25 min each at 25°C ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Immobilon-FL, EMD Millipore, Billerica, MA) and probed using antibodies against tau (Tau-5 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used included anti-rabbit glial fibrillary acidic protein (GFAP, Sigma, 1:400), mouse anti-Zrf1 (Gfap ...
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bioRxiv - Molecular Biology 2020Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Reactive proteins were detected with either the HRP-conjugated goat anti-mouse IgG (Sigma) or donkey anti-rabbit IgG (1:3000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were cleared by centrifugation and precleared with PureProteome Protein A magnetic beads (Millipore) for 2 hours at 4°C ...
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bioRxiv - Genetics 2019Quote: ... filtered through a Millex-HV 0.45 μm low-protein-binding membrane (Millipore, Darmstadt, Germany), divided into aliquots ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-P; Merck Millipore, MA, USA) using a Trans-Blot® SD semi-dry transfer cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were immunoprecipitated overnight at 4 °C with PKM2 antibody (SAB4200105, Sigma) or normal rabbit IgG (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Ten milligrams of protein was incubated with 4 μL α-myc antibody (Sigma M4439) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Twelve milligrams of protein was incubated with 4 μL α-myc antibody (Sigma M4439) at 4°C overnight while rotating ...
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bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of GFP-SGS3 protein was induced in Escherichia coli Rosetta (DE3) (Novagen) by adding 0.1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2019Quote: ... followed by an overnight incubation with PureProteome Protein A/G mix magnetic beads (Millipore). Samples were washed as indicated by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... Green fluorescent protein (GFP) expression was monitored by flow cytometry (FACSCalibur Guava 8HT [Millipore]) starting on day 12 ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were then transferred to Polyvinylidene difluoride (PVDF) membrane (Immobilon®-P, Millipore, IPVH100010). Membranes were blocked with 5% non-fat dry milk in TBS-T (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole cellular protein was extracted with RIPA buffer containing 1 mM Na3VO4 (Sigma-Aldrich) and protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected by Western blot using HRP-conjugated mouse anti-FLAG (Sigma, A8592) and rabbit polyclonal anti-GFP (Chen et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Protein-containing fractions were pooled and concentrated using Amicon centrifugal units (MWCO 30,000, Millipore), centrifuged (17,000xg ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were then resolved with SDS-PAGE and blotted to PVDF transfer membrane (Millipore). The presence of the proteins of interest was tested by immunodetection using rat anti-HA-peroxidase (3F10 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (pore size, 0.45μm; Millipore Sigma, USA) and blocked with 5% nonfat dry milk in Tris-buffered saline (20mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... Protein was concentrated using a Amicon Ultra 15mL 3K NMWL centrifugal filter (Millipore Sigma).
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bioRxiv - Physiology 2019Quote: Proteins were extracted from tissues using Ripa buffer and protease inhibitor cocktail (Sigma-Aldrich). Tissues homogenates were centrifuged twice for 20 min at 12700 rpm and supernatants were quantified with BCA pierce kit (ThermoScientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Captured protein complexes were eluted with 30μl of 1N Ammonium Hydroxide (#320145, Sigma-Aldrich), 5mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated in a 10k MWCO Amicon-Ultra centrifugal filter (Millipore Sigma) and loaded onto a Superdex 200 HiLoad 16/60 gel filtration column equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... We induce protein expression by adding 1 mM isopropyl β-d-1-thiogalactopyranoside (Sigma) when the cell density reaches an ODλ=600nm of 0.6 ~ 0.7 ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2021Quote: ... the specific proteins were visualized with a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins for size-exclusion column calibration were purchased from Sigma-Aldrich (St. Louis, MO). 5-aminolevulinic acid was purchased from Carbosynth (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were concentrated by centrifugation with a 10 kDa cut-off membrane (Merck Millipore) to a volume of 300 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were transferred to PVDF (polyvinylidene difluoride) membranes (0.2 μm pore size, Millipore) by immersion transfer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Protein lysates were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Millipore). After the membranes were blocked using 5% skimmed milk (Megmilk Snow Brand ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was concentrated to 5 mg·ml−1 in an Amicon ultracentrifugation device (Millipore) with 10 kDa cutoff and directly used for further studies or supplemented with 40% glycerol ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted with Wash Buffer containing 0.2 mg/ml 3xFLAG peptide (Sigma). CSB proteins were further purified by gel filtration (Superdex 200 Increase ...
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bioRxiv - Neuroscience 2020Quote: ... A pre-clear step was performed by adding protein A-agarose beads (Merck Millipore) to the lysates ...
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bioRxiv - Neuroscience 2020Quote: Mouse anti-Myelin Basic Protein (MBP-SMI-99, Millipore, reference NE1019, 1/1000 dilution), rat anti-Myelin Basic Protein (BIO-RAD ...
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bioRxiv - Biochemistry 2020Quote: Solutions of intact and deglycosylated proteins were passed through the ZipTip C18 microcolumns (Millipore), washed and eluted according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... the target proteins were transferred onto PVDF membranes (Millipore Durapore, 0.45 μm pore size). The membranes were washed ...
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bioRxiv - Neuroscience 2019Quote: ... The antibody was captured using 30 μl of 50% Protein G Sepharose beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...