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Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... isolated heart mitochondria were solubilized with digitonin (Sigma Aldrich, 6g/g digitonin/protein ratio) and resolved on a 4-12% Bis-Tris polyacrylamide gradient gel (Genscript) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted by adding 100 µl of 200 µg/ml 3xFLAG peptide (Sigma) in TBS and incubating with rotation at 4°C for 30 min.
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bioRxiv - Genetics 2021Quote: ... antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma) or mouse anti-Flag (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was incubated with Protein G-coupled magnetic beads (Sigma, MO, USA) that captured with anti-GFP for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... Protein quantification was carried out using BCA (Bicinchoninic Acid, Sigma-Aldrich, St. Louis, MO) method taking bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were expressed in Sf9 insect cells and purified on M2 agarose (Sigma Aldrich) as described (J ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Molecular Biology 2022Quote: Crude protein extracts were mixed with 1% Sodium Dodecyl Sulphate (SDS; Sigma-Aldrich, USA) and boiled for 1 min ...
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bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
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bioRxiv - Cell Biology 2022Quote: ... were coated with the following ECM proteins when noted: gelatin (Sigma Aldrich, catalog # G2500) (0.1% (w/v ...
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bioRxiv - Genetics 2022Quote: ... Immune complexes were collected by centrifugation and cleared using Protein A agarose beads (Millipore) and incubated for 1.5 h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein extracts were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). Tubulin was used as a loading control (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated using a 10 kDa molecular weight cut-off concentrator (Millipore) and loaded onto a HiLoad 16/600 Superdex 75 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of Magna ChIP protein A magnetic beads (16–661, Millipore, Molsheim, France) were added for 1h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was concentrated with a 30-kDa molecular mass cut-off concentrator (Millipore) by centrifugation for 5 min at 2,500 x g ...
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bioRxiv - Developmental Biology 2022Quote: ... and separated proteins were transfer to polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Fisher Scientific). Total lane protein was then determined via Ponceau stain (0.1% Ponceau-S in 5% acetic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Small molecules/ proteins were added to extracts as follows: 1 µM MLN8237 (Sigma-Aldrich) (or 0.5% DMSO ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was concentrated by ultrafiltration using an Amicon 50K filter unit (Millipore). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were analyzed on SDS-PAGE and concentrated with Amicon Ultra concentrators (Millipore), aliquoted and stored at −80 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated by a 30kDa centrifugal filter unit (Millipore Sigma Cat# ufc903024), and stored in 50% glycerol PBS at −20°C.
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bioRxiv - Immunology 2019Quote: ... mouse anti-MAP2 (microtubules associated protein 2) antibody (Cat. MAB378, Millipore, Boston, MA, USA), anti-ZIKV-E antibody (Cat ...
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bioRxiv - Physiology 2020Quote: ... The protein concentration was assessed using bicinchoninic acid assay (BCA, BCA1-1KT, Sigma Aldrich). A total of 10-40 μg of protein was loaded on 8-14% acrylamide gel ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was then transferred on to a polyvinylidene fluoride membrane (PVDF) (Millipore, USA) using a semi-dry transfer system (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Variants were subcloned from the pEDA3 background into protein expression plasmid pET-29b(+) (Novagen) or into the constitutive expression plasmid pEDA2 (Wrenbeck et al ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins in the gels were either visualized by silver staining (ProteoSilver; Sigma Aldrich) or transferred from the gel to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... 20µg of digested protein were desalted using 8µg C18 ZipTip (Millipore, Bedford, MA, USA). Approximately 1.5µg protein was injected into Acquity M-Class UPLC (Waters) ...
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bioRxiv - Plant Biology 2019Quote: ... 1 mL of protein extraction was applied to M5 anti-FLAG magnetic beads (Sigma). The beads were then boiled and the resulting fraction was run on a 12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2019Quote: For protein half-life analysis cells were treated with 10μg/ml cyclohexamide (Sigma Aldrich) for 2 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were then transferred onto a PVDF membrane (Immobilon®-P Transfer Membrane, Millipore) via wet transfer and blocked in 5% skim milk/Tris-buffered saline (TBST ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were transferred on to a membrane (Millipore IVD HYBOND–C Extra membrane) by slot transfer under vacuum ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted the soluble protein from the damselflies using 0.1 M NaOH (Sigma-Aldrich) as a lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred from SDS-PAGE gels to PVDF-membranes (Immobilon-P, Millipore 0.45µm) in transfer buffer (per 1 l ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing cleaved proteins were concentrated using a 50K MWCO concentrator (EMD Millipore) to 1 mL ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Neuroscience 2019Quote: ... Protein synthesis was inhibited by including 20 μg/mL cycloheximide (Sigma-Aldrich, Cat #C4859) in the imaging medium ...
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bioRxiv - Biophysics 2019Quote: ... 15 μL diluted proteins were mixed with 1 μL 100x Sypro Orange (Sigma-Aldrich) and loaded in LightCycler 480 Multiwell Plate 96 (Roche ...
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bioRxiv - Plant Biology 2019Quote: ... Purified proteins were then concentrated using Amicon Ultra concentrators (molecular-weight cutoff 10,000; Millipore) and purity and structural integrity was assessed by SDS-PAGE and Right Angle Light Scattering (RALS) ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred onto a PVDF membrane (0.45 μm pore size, Immobilon-P, Millipore) using semidry transfer (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins were incubated with 50 µL agarose conjugated with anti-Myc antibody (Sigma) for 2 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein content was determined using the Bradford reagent (Serva) with BSA (Sigma-Aldrich) as a standard (Bradford ...
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bioRxiv - Cell Biology 2019Quote: ... total protein content was stained using 0.1% (w/v) Ponceau S (Sigma-Aldrich, P3504) in 1% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... The delipidated NPC2 proteins were incubated with increasing concentrations of cholesterol (>99%) (Sigma Aldrich), in DMSO ...
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were resolved by 10% SDS-PAGE and electrotransferred onto polyvinylidene fluoride membrane (Millipore) in transfer buffer (10 mM CAPS ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...