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Citations for Millipore Sigma :
7201 - 7250 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated by Amicon Ultra Centrifugal Filter 50 kDa (Merck Millipore) and purified on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: We conducted SEC on the SEC protein standards (molecular weights 12.4-200 kDa; Sigma) including β-amylase from sweet potato (200 kDa) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was concentrated by centrifugal filtration (Amicon, EMD Millipore, 10-kDa MW cutoff) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein recovery was maximized by adjusting the concentration of imidazole (Sigma cat# l5513-5G) as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were transferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Sigma-Aldrich) at 100V for 1 hour on ice in NuPAGE Transfer Buffer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-FL Transfer Membrane (Millipore). The membranes were blocked in PBS-T (PBS containing 0.05% Tween 20 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Total cell protein was extracted with lysis buffer (RIPA, Sigma-Aldrich, catalog no. R0278) containing 1% protease-phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Proteins separated by SDS–PAGE were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100 V for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein ubiquitination was tested by immunoblots and FLAG-HRP antibody (Sigma, Catalog number A8592).
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bioRxiv - Microbiology 2022Quote: ... the total protein concentration of each sample was measured via Bradford assay (Sigma Aldrich). Samples were subsequently diluted to a final concentration of 0.5 µg total protein/µl ...
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bioRxiv - Microbiology 2022Quote: ... The TS fraction was concentrated using Amicon protein concentrators with a 10KDa cutoff (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Not protein depletions were obtained by addition of auxin (3-indoleacetic acid, Sigma-Aldrich I2886 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed with 50 μL of 1 × BugBuster Protein Extraction Reagent (Sigma). Lysates clarified at 21 ...
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bioRxiv - Neuroscience 2022Quote: ... and 15 μL of Protein-G Sepharose (Millipore/Cytiva Cat#GE-17-0618-05), washed three times in ice-cold wash buffer (0.1% Triton X-100 in 150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the Bradford reagent (Sigma Aldrich, St. Louis, MO, USA). Equal amounts of proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by using Trans-Blot Turbo™ Transfer Pack (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The DNA protein complex was immunoprecipitated with either anti-FLAG monoclonal antibody M2 (Sigma) or anti-σ70 antibody (BioLegend ...
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bioRxiv - Plant Biology 2022Quote: For NR activity measurements with the purified protein the procedure was adapted from Sigma quality control tests for product N7265 based on (Gilliam et al. ...
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bioRxiv - Physiology 2023Quote: ... protein concentrations of pancreatic samples were quantified using a Bradford assay (Sigma-Aldrich, B6916). Insulin and SST values were then normalized to protein concentration ...
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bioRxiv - Plant Biology 2023Quote: ... The peak fraction of each BE protein was desalted on PD10 desalting column (Sigma) equilibrated in 20 mM Tris/HCl pH 7 ...
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bioRxiv - Genomics 2022Quote: HC protein fragments were cleaved by thrombin (40 mU/μL) (EMD Millipore, 605157-1KU) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... the specific proteins were visualized using a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) and LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein complex was eluted in Strep Buffer supplemented with 2.5 mM desthiobiotin (Sigma) at 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were then digested with 10 µg/mL proteinase K (Sigma-Aldrich) at 55 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins on gels were transferred on PVDF membrane (Immobilon-P, Millipore, Cat no. IPVH00010) and blocked with 5% non-fat dry milk (Scientific ...
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bioRxiv - Pathology 2022Quote: ... the incubated protein mixture was collected and centrifuged using centrifugal filters Utracel-100K (Millipore) at 500 × g for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... The total ubiquitinated proteins were detected by anti-FLAG (F-3165, 1:5,000, Sigma) and α-MYC (ab32 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were resolved by and blotted on Transfer membrane (Immobilon-P, Merck Millipore IPVH00010). Membrane blocking (5% BSA/TBS ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using 10 kDa cutoff Amicon Ultra Centrifugal Filters (Merck Millipore) and stored in 50% v/v glycerol at -20 °C until further use.
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bioRxiv - Pathology 2024Quote: ... the resolved protein samples were transferred onto a PVDF membrane (Merck Millipore Ltd., USA) through a Bio-Rad electrophoretic blotting apparatus ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore). The protein concentration was measured on a Nanodrop 1000 spectrophotometer at 280 nm using the extinction coefficient of Trp and Tyr residues in the GST-LC3B sequence (48820 cm-1M-1).
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Bioengineering 2024Quote: ... iFluor 647-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE178-100UG); iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake ...
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bioRxiv - Bioengineering 2024Quote: ... iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE176-100UG); IRDye 680RD Donkey anti-goat (Western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were semi-dry blotted onto a 0.2 µM nitrocellulose membrane (Sigma cat GE10600001) and protein levels were detected by specific antibodies directed against ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30K MWCO concentrator (EMD Millipore) to 1 ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore). All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... 80 μl agarose protein G with salmon sperm DNA slurry (Millipore Cat. 16-201) was added for pre-clearance ...
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bioRxiv - Microbiology 2024Quote: ... 80 μl agarose protein G with salmon sperm DNA slurry (Millipore Cat. 16-201) was added for pre-clearance ...
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bioRxiv - Cell Biology 2024Quote: mESC protein samples were isolated from mESC pellets using ice-cold RIPA buffer (Sigma) in the presence of freshly added 1% protease and phosphatase inhibitor cocktail (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 protein was extracted from cells using RIPA buffer (Sigma-Aldrich, UK. Cat # R0278) using the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... biotinylated proteins bound to streptavidin beads were resuspended in 100 mM ammonium bicarbonate (Sigma) and digested with 2 µg Trypsin (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...