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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... A solution of graphene oxide (GO, 2 mg mL−1, Sigma-Aldrich) was bath sonicated to evenly resuspend it ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM MgCl2 and 2 mM CaCl2 (all chemicals from Sigma-Aldrich) at the flow rate of 10 ml/min for 2 min to remove blood ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was diluted 1:2 in nuclease free water (SIGMA-Aldrich) and real time quantitative PCR (RT- qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... After a saturation step with Gamma Immune (Sigma Aldrich, dilution 1 :2), cells were labeled with antibodies detecting the following cell-surface markers ...
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bioRxiv - Microbiology 2024Quote: ... 1% w/v sodium choleate and 2% w/v glucose (Millipore Sigma) was used [36] ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in a 2:1 solution of dichloromethane (DCM; Sigma 270997) and methanol for 3 h followed by 2× 15-min washes 100% DCM ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were washed 2 x 1 hour in 0.2% Tween-20 (Sigma) in 1X PBS with 10 µg/ml heparin (Fisher ...
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Coverslips were washed with PBS for 5 minutes and then fixed in 4% paraformaldehyde for 30 minutes and permeabilized using 0.5% TritonX-100 (Sigma-Aldrich) for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... They were fixed with a mixture of 4% paraformaldehyde and 5% sucrose for 10mins and permeabilised using 0.1% Triton X-100 in PBS (Sigma-Aldrich) for 5min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... 35.5 mM sucrose and 5 mM EGTA) and fixed in either 4% formaldehyde for 20 min or Bouin’s fixative (Sigma-Aldrich) for 2 min ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotted PDVF membranes were blocked in 5% (w/v) non-fat milk powder or 4% (w/v) Bovine Serum Albumin (BSA, Sigma) in PBS-T ...
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bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
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bioRxiv - Microbiology 2019Quote: ... J774 murine macrophage-like cells were cultured for a minimum of 4 passages in T75 tissue culture flasks at 37°C 5% CO2 in DMEM (High glucose, Sigma) supplemented with 10% Fetal Bovine Calf Serum (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... The spinal cords were then extracted and fixed in 4% paraformaldehyde (PFA) and 5% saturated picric acid (Sigma-Aldrich, P6744) in phosphate buffer saline (PBS ...
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bioRxiv - Bioengineering 2021Quote: ... a volume of 4 mL of top agar (TSB + 0.75% bacteriological agar, Scharlau) and 5 mM calcium chloride (Sigma-Aldrich) were added to the phage dispersion mixed with the bacteria ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µg/ml bovine insulin [Sigma], 4 µg/ml heparin [Sigma], 20 ng/ml basic fibroblast growth factor [bFGF, Sigma] ...
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bioRxiv - Cancer Biology 2020Quote: SUM159PT spheres were dissociated by TrypLE™ and plated in mammosphere media (DMEM-F12, 10 ml/500 ml B27 [Invitrogen], 5 µg/ml bovine insulin [Sigma], 4 µg/ml heparin [Sigma] ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 ppt APS, 1.5 ppt TEMED, and (optionally, for thick tissue slices) 15 ppm 4-hydroxy TEMPO (4HT, Sigma, 176141). Monomer solution was made by combining all components of gelation solution except APS ...
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bioRxiv - Genomics 2021Quote: ... Samples were then centrifugated at 500g for 5 minutes at 4°C and then were resuspended in 1mL Nuclei PURE Storage Buffer (Nuclei PURE storage buffer, Sigma). Sample washing was performed until the supernatant cleared ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with a cytometry buffer solution (PBS with 5 % FBS) then fixed with a 4 % formaldehyde (Sigma-Aldrich) for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Fat bodies were dissected from larvae at 5 or 12 days AEL fixed in 4% PFA and counterstained with Nile Red (Sigma), Phalloidin-488 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... adult DN mutant and Ctrl sibling zebrafish were bathed for 24 h in the presence of 5 μM 4-HT (H7904; Sigma) made from a 10 mM stock solution dissolved in 100% ethanol at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Plant Biology 2021Quote: ... Then the proteins were concentrated to 5∼10 mg/ml using the 30 kDa cut-off Amicon Ultra-4 centrifugal filter unit (Millipore), and stored at −80 °C for crystallization ...
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bioRxiv - Cancer Biology 2020Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Mixed peptides were subjected to a reverse phase clean-up step (OASIS HLB 96-well μElution Plate ...
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bioRxiv - Immunology 2020Quote: ... CD11c-DTR BM or CD11c-DTR Rag2−/− BM chimeric mice were injected (i.p.) with 4-5 ng/g body weight diphtheria toxin (DT, Sigma-Aldrich) every other day for the indicated time ...
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bioRxiv - Neuroscience 2020Quote: ... and were either incubated with primary antibodies in blocking solution (5% donkey serum and 0.5% Triton in PBS) or were embedded in 4% agar (low-gelling agarose, Sigma Aldrich) and sectioned at 120 μm in the vibratome ...
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bioRxiv - Immunology 2020Quote: 8 to 12-weeks old female C57BL/6j were inoculated subcutaneously with 5×105 B16-F10-OVA and conditioned 4 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Developmental Biology 2020Quote: ... After blocking overnight with pH 8.0 Tris-buffered saline with 0.1% Tween20 containing either 4% non-fat dry milk (Carnation) or 5% bovine serum albumin (Sigma-Aldrich), membranes were incubated with the appropriate primary antibody diluted in blocking buffer (4% milk or 5% BSA in 1X TBST ...
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bioRxiv - Microbiology 2022Quote: ... The fluid was centrifuged at 500xg for 5 min at 4°C and resuspended in RBC lysis buffer (Sigma, R7757) for 5 min at room temperature ...
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Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteinsbioRxiv - Biochemistry 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed ...
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bioRxiv - Biochemistry 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Immunology 2022Quote: ... 8-15-weeks-old female C57BL/6J/Ly5.2 were inoculated subcutaneously with 0.5 x 106 B16-F10-OVA and conditioned 4 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 7 ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were resuspended in 0.4 ml of sonication buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Sigma Aldrich]) and subjected to sonication at 4.5-micron amplitude for 7 cycles of 15 seconds on/15 seconds off ...
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bioRxiv - Immunology 2022Quote: ... the cells were firstly cultured for 4 h in a 37°C/5% CO2 incubator with 50 ng/ml PMA (Sigma) and 1 mM ionomycin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei and other debris were pelleted at 2,000 g for 5 min at 4°C and the supernatant was filtered through three layers of 100 μm pore nylon mesh (Millipore), and a 5 μm pore PVDF syringe filter (Millipore) ...
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bioRxiv - Genomics 2024Quote: ... Embryos were then incubated overnight at 37°C under 5% CO2 in humidified air in 4-well culture dishes containing KSOM media (Millipore). Experiments were approved by the SIMR IACUC and were performed following the committees’ guiding principles.
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tg(-3.5ubb:loxP-GFPloxP-mCherry)cz1701Tg embryos were incubated in embryo media containing 5 μM (Z)−4-Hydroxytamoxifen (Sigma-Aldrich H7904) starting at 4-4.5 hours post-fertilization (hpf ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were reconstituted in 100 µL and 2 µL of the sample was injected from a 4 °C autosampler into a ZIC-pHILIC 150 × 2.1 mm 5 µm particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 537-98-4] were added to the media from 25 mg/mL stock (in ethanol [Sigma Aldrich, 64-17-5]), and the final ethanol concentration was adjusted to 1.86% v/v for all conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in 1xPBS for 5 minutes and coverslipped with 75µl of mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma, 2370).
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bioRxiv - Immunology 2022Quote: ... RT complete IntestiCult containing 25μg/ml was added, and enteroids were incubated (37°C, 5% CO2) until fixation with 4% paraformaldehyde (PFA; Sigma Aldrich) at 4h p.i ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... 4- to 5-week-old donor rats were intraperitoneally injected with 30 units of equine chorionic gonadotropin (G4877, Sigma-Aldrich), followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 5 times in PB 0.1 M and mounted with a mounting solution (Mowiol 4-88, Sigma-Aldrich).