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Citations for Millipore Sigma :
7251 - 7300 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Ovary of both medaka and trout were fixed overnight at 4°C by 4% paraformaldehyde (PFA) in 0,01 M phosphate buffer saline pH 7.4 (PBS, Sigma-Aldrich P4417). Ovaries were washed in PBS and conserved in PBS + 0.5% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 4% paraformaldehyde in 0.1 M phosphate buffer (4% PFA-PB; pH 7.4; Sigma-Aldrich, St Louis, MO, USA). Lumbar spinal cords were collected and placed into 4% PFA-PB for 2 hours at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sma-CreERT2 or Cx40-CreERT2 males were crossed with R26-Confetti females and low doses of 4-Hydroxytamoxifen (4-OHT H6278, Sigma) was injected intraperitoneally to pregnant females in a single injection ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were subsequently fixed in paraformaldehyde (PFA) 4% with 4% sucrose for 10 min and permeabilized with 0,1% Triton X100 (Sigma-Aldrich) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: 4% hydrogel monomer solution (HMS) was prepared for perfusion by mixing 40% (w/v) acrylamide (4% final concentration; V900845, Sigma), 2% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated at 37°C for 30 minutes prior to fixation with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: Cells were washed with phosphate buffered saline (PBS) and fixed for 30 min at room temperature with 4% paraformaldehyde (containing 4% sucrose; Sigma). Cells were permeabilized in 0.5% NP40 in PBS for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... NestinCreERT2 - /+ TDT +/+ IR fl/fl generated secondary spheres were allowed to grow for 4 DIV and then 0.5 µM of 4-hydroxy tamoxifen (Sigma-Aldrich) or vehicle (ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... Induction of recombination in Ai9 mice electroporated with Cre inducible plasmids was achieved by intraperitoneal injection of pregnant dams with the faster-acting and shorter-lived tamoxifen metabolite 4-hydroxy-tamoxifentamoxifen (4-OHT) (Sigma) (1 mg/20 g of body weight ...
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bioRxiv - Immunology 2022Quote: ... 0.6μL was deposited on a thin layer of an air-dried saturated solution (0.6μL) of the matrix alpha-cyano-4-hydroxycinnamic Acid (4-HCCA, Sigma Aldrich, France) in pure acetone ...
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bioRxiv - Biochemistry 2019Quote: ... The selected fractions of the SEC peak corresponding to the purified Dispatched were concentrated to 4 mg/ml using an Amicon Ultra-4 concentrator (100 kDa cut-off, Millipore).
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bioRxiv - Microbiology 2019Quote: ... aliquots of 100 μl of the supernatants were mixed with an equal volume of 4 % w/v Ehrlich’s reagent 4-(dimethylamino) benzaldehyde (156477, Sigma-Aldrich) in glacial acetic acid (Fisher Chemical A/0360/PB15) ...
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bioRxiv - Cancer Biology 2019Quote: ... The vDISCO pipeline was started immediately or whole mouse bodies were stored in PBS at 4 °C for up to 4 weeks or in PBS containing 0.05% sodium azide (Sigma, 71290) for up to 6 months.
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bioRxiv - Microbiology 2019Quote: ... The cells were cultured for 24 hrs post transfection before addition of fresh media containing 100 µM 4-thiouridine (4-SU, Sigma), a photo-crosslinkable ribonucleoside analogue ...
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bioRxiv - Bioengineering 2020Quote: ... the brain was kept in 4% PFA overnight at 4□°C and then placed into PBS containing 0.02% sodium azide (Sigma S8032). The brain was serially sectioned into 50 μm transverse slices on a Campden Instruments 5100mz vibratome ...
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bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and then 4 fractions of 4mL were concentrated 8 times using 10 kDa Amicon Ultra-4 10K Centrifugal filters (Millipore) after centrifugation (20 min ...
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bioRxiv - Microbiology 2020Quote: ... Flagella preparations were incubated at 70°C for 15 min then filtered by centrifugation at 5000 × g at 4°C for 30 min using 100 kDa 4 ml filter units (Millipore). Filtrates were confirmed by size-exclusion HPLC to be exclusively monomeric.
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bioRxiv - Microbiology 2021Quote: ... GM130 (12480, cell signaling), PI(4)P (Z-P004), PI(4)KB (06-578, Milipore) and anti-actin (A5316, Sigma). Secondary antibodies were as followed ...
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bioRxiv - Neuroscience 2020Quote: ... Srff/f and Srff/fCaMKCreERT2 pups were intraperitoneally injected every other day with three doses of 0.25 mg 4-hydroxytamoxifen (4-OHT; Sigma, #7904) or one dose of 0.75 mg of this compound in sunflower oil ...
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bioRxiv - Cell Biology 2021Quote: Oocyte live cell imaging was done in 4-well Labtek chambers (ref. 155383) in 4 μl drops of M16 medium covered with mineral oil (Sigma) in a 5% CO2 environmental microscope incubator at 37 °C (Pecon) ...
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bioRxiv - Cell Biology 2021Quote: Lgr5-EGFP-IRES-creERT2/RCL-tdT small intestinal organoids were treated in vitro with 100 nM of 4-Hydroxitamoxifen (4-HT) (Sigma) for 48 h to induce the expression of tdTomato in Lgr5+ cells ...
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bioRxiv - Neuroscience 2022Quote: ... Ai14 double transgenic mice were generated and received virus injection of rAAV9-N-RAM- d2tTA-eYFP with an identical schedule as described above except of 4-OHT injection (50 mg/kg, 4-Hydroxytamoxifen, H7904, Sigma) immediately after fear conditioning ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Microbiology 2024Quote: ... Tissues were fixed in 4% paraformaldehyde for 24 hours at 4 °C and washed with Dulbecco’s phosphate-buffered saline (DPBS, Sigma, USA). After dehydrated through a graded ethanol series to absolute ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were fixated at DIV42 with 4% paraformaldehyde/4% sucrose for 15 min and permeabilized with 0.2% Triton X-100 (Sigma Aldrich) in PBS for 10 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Rosa-Tomato mice in diestrus cycle were injected intraperitoneally with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich, #H6278, St. Louis, MO) to induce CreER recombinase activity ...
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bioRxiv - Immunology 2024Quote: ... or alternatively plus IL-4 (4ng/ml) or IL-4 and lipopolysaccharide (LPS) (10μg/ml) (Sigma-Aldrich, St. Louis, MO) for 2.5 days.
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bioRxiv - Microbiology 2023Quote: ... cells were fixed overnight in 4% paraformaldehyde in PBS at 4°C and plated onto 0.1% poly-L-lysine (Sigma-Aldrich) coated cell slide ...
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bioRxiv - Cancer Biology 2023Quote: ... in vitro culturing was continued in fresh medium containing 500 nM 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich, St. Louis, MO), which was changed daily ...
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bioRxiv - Bioengineering 2023Quote: ... solution for 4 h at 60°C and then dialyzed at 4°C against pure water for 4 days using cellulose dialysis tubes (MWCO 3.5 kDa, Sigma Aldrich). Subsequently ...
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bioRxiv - Developmental Biology 2023Quote: ... and in vitro assays on SK-MEL-28 cells were as follows: 4-Hydroxyanisole (4-HA, Sigma-Aldrich, MO, USA), the canonical Wnt pathway antagonist IWR-1 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were incubated overnight at 4°C with primary antibodies diluted in antibody solution containing 4% NDS (Sigma-Aldrich) and 0.1% Triton-X (Sigma- Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... This cocktail was allowed to rotate at 4 °C for 4 hours before adding 50 μL of streptavidin agarose resin (Millipore) overnight with continued rotation at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich), α-ZC3HAV1 (GTX120134 ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich) or with isotype control (12-370 ...
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bioRxiv - Biochemistry 2023Quote: ... TEV-digested histones were then concentrated up to 4 mg/mL with ultrafiltration using Amicon YM50 membrane (MWCO 50 kDa) at 4 °C (Millipore). The concentrated sample was then injected onto a Superdex 200 10/300GL column ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were postfixed in 4% PFA and stored at 4°C and sections were counterstained with Nuclear Fast Red (Sigma). Embryos and sections were imaged using a Nikon SMZ800 stereo microscope.
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bioRxiv - Systems Biology 2024Quote: ... and kept at 4°C for a maximum of 4 hours until plasma was separated via centrifugation (2600g for 10 minutes at 4°C) (Sigma 3-16KL ...
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bioRxiv - Cancer Biology 2023Quote: ... for 30 minutes at 4 degrees before fixation and permeabilisation with 4% PFA and 0.1% TritonTM X-100 (Sigma-Aldrich) respectively ...
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bioRxiv - Biophysics 2024Quote: ... HaCaT cells with around 50% confluency were infected by culturing in the adenoviral conditioned media with 4 μg/mL polybrene (28728-55-4, Sigma) for 7h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT) ...
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type ICR females (3-4 weeks old) were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a tip resistance of 3 to 4 MΩ were filled with (in mM): K-gluconate (135 mM, Sigma; G4500-100G), EGTA (0.2 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Surgical specimens were washed twice in phosphate buffered saline (PBS) and fixed for approximately 3 hours in 4% paraformaldehyde (Sigma, St. Louis). Tissue was washed two times in PBS and incubated at 4°C for 3-5 days in 30% sucrose ...
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bioRxiv - Immunology 2019Quote: ... 24 and 48 hours and then fixed with 4°C methanol for 3 minutes and stained with safranin (Sigma-Aldrich, Gilligham, UK) for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: Immunoprecipitations were set up with 100 µl solubilised chromatin (roughly equivalent to 4 × 106 cells) and 3 µg of an anti-acetylated histone H3 antibody (Millipore, 06-599) in a total of 1 ml Native ChIP Incubation Buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 3 days at 37°C before overlay medium was removed and cells were fixed with 4% PFA (Sigma-Aldrich, MA) for 30 minutes at room temperature ...