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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... RPMI 1640 complemented with indicated inhibitors was replaced daily for 3 days before overnight fixation with formalin at 4°C (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Molecular Biology 2024Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous or 3-4 days administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... diluted in corn oil (CO, used as vehicle) at 3 mg/kg for 4 days while CO mice received corn oil only (Sigma Aldrich). One cage of n=6 mice per group was used ...
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bioRxiv - Cell Biology 2023Quote: ... The AAV was concentrated by centrifugation at 2000g for 3-5min at 4°C using an 100k Amicon Ultra Centrifugal Filter Unit (Millipore #UFC810024). After centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... Sub culturing was done once in 3-4 days and involved pelleting and washing cells with PBS followed by incubation with accutase (Sigma-Aldrich) and repeated pipetting in NSM medium to dissociate the spheroids ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves were harvested and fixed for 1 h in 4% paraformaldehyde (PFA) followed by 15 min immersion in a 3% sodium fluorescein (NaFlu, molecular weight: 376 Da; Sigma Chemicals) in saline solution 2 ml of Evans blue albumin (EBA ...
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bioRxiv - Systems Biology 2024Quote: ... morphine paradigm were communally housed (3-4 mice per cage) and had access only to water supplemented with either sodium butyrate (Sigma-Aldrich) or pH-matched (9.30 - 9.50 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dried samples were first resuspended in a 1:1 v/v mixture of a 1% solution of N,N-diisopropylethylamine (Sigma) and a 1% solution of pentaflurobenzylbromine (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... BubR1 5’GAUGGUGAAUUGUGGAAUAdTdT3’) or luciferase (5’ CGUACGCGGAAUACUUCGAdTdT 3’) was synthesized from Sigma and used for the RNAi.
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bioRxiv - Developmental Biology 2021Quote: ... cytochalasin D (Sigma, 0.5 µM for 1h), monastrol (SelleckChem ...
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bioRxiv - Neuroscience 2022Quote: ... 1h with DAPI and TRITC-phalloidin (Sigma), then mounted with Vectashield mounting medium (Vector Laboratories) ...
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bioRxiv - Developmental Biology 2021Quote: ... At the relevant timepoint embryos were fixed overnight at 4°C using 4 % (w/v) paraformaldehyde (Sigma-Aldrich, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of WT LcαE7 and the Gly137Asp LcαE7 variant was determined by a competition assay between the native-substrate analogue 4-nitrophenol butyrate (4-NPB, Sigma) and the boronic acid compounds ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 4% methanol-free formaldehyde (Thermo, 28906) and permeabilized in .4% Triton X-100 (Sigma, T8787). The Click-iT Cell Reaction Buffer Kit was then used to “click” the biotin alkyne (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... the cell culture media was replaced with fresh media and supplemented with 1μM 4-OHT (4-hydroxytamoxifen, Sigma, H7904) for both control and mutant cells ...
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Cell Biology 2021Quote: β-catenin knock-out in βfl/- cells was induced through the addition of 0.1 μg/mL 4-hydroxy-tamoxifen (4-HT, cat. num. H6278, Sigma) to the media every 24 hours for 72 hours ...
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bioRxiv - Neuroscience 2022Quote: ... data was separated into pre-defined frequency bins (Delta 0.5-4 Hz, Theta 4-8 Hz, Alpha 8-13 Hz, Sigma 12-15 Hz ...
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bioRxiv - Cell Biology 2023Quote: ... and fumaric acid-1,4-13C2,2,3-2H2 (≥99 atom% 13C, ≥98 atom %2H, CAS 100858-52-4, cat. no. 752576) from Sigma Aldrich. All chemicals were used as received.
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bioRxiv - Neuroscience 2024Quote: ... followed by transcardial perfusion with 1X phosphate-buffered saline (PBS) and then 4% paraformaldehyde (Sigma-Aldrich, 30525-89-4) diluted in 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stored for up to 4 months at 4°C in 0.5% sodium azide (Sigma-Aldrich, Cat#S2002) in 1x PBS to prevent bacterial growth ...
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bioRxiv - Cancer Biology 2024Quote: ... Topical 4-hydroxytamoxifen (4-OHT; 25 mg/mL or 75 mg/mL in DMSO; 98% Z-isomer, Sigma-Aldrich) was administered to pups on their back at ages P2-P4 ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old Col-0 and lnccobra1-2 seedlings were crosslinked in PBS with 1% formaldehyde (v/v) (Sigma-Aldrich; St. Louis, MO, USA) added and placed under vacuum for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... for 2 h at room temperature and then blocked for 1 h at room temperature with 5 % neonatal calf serum (Millipore Sigma, St. Louis, MO) in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... frozen leaf samples were ground in liquid nitrogen and incubated in 4 ml lysis buffer (50 mM Tris-HCl pH7.5, 150 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT, 2% PVPP) at 4 °C for 15 min using a rotator to form a homogeneous suspension ...
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bioRxiv - Microbiology 2020Quote: ... Strains were routinely grown under yeast conditions (media at 30°C) in YPG (1% yeast extract, 2% Bacto peptone, 2% glucose) or under filament-inducing conditions using RPMI medium (Sigma, St. Louis, MO) with MOPS (morpholinepropanesulfonic acid ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were stained with DAPI (2 μg/ml, room temperature, 5 min, Sigma, St. Louis, Missouri, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Microbiology 2021Quote: 20 μg of fraction 19 were reduced with 5 mM Tris(2-carboxyethyl)phosphine (646547, Sigma Aldrich) for 60 min at 65 °C ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... larvae were placed on nematode growth medium (NGM) plates containing 5-fluoro-2’deoxy-uridine (FUDR, Sigma) (75 μM ...
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bioRxiv - Microbiology 2020Quote: ... Continuous culture was typically carried at 2-5%-hematocrit in RPMI-1640 (Sigma Aldrich, Cat. No. R6504) supplemented with HEPES ...
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bioRxiv - Immunology 2022Quote: Total RNA was collected from cultured cells treated with 100 nM 5-aza-2⍰-deoxycytidine (decitabine; Sigma) or splenocytes from a naïve C57BL/6 mouse using NucleoSpin RNA Plus (Takara) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were combined with NuPage LDS sample buffer 4x (#2083421) and 5% 2-Mercaptoethanol (Sigma Aldrich #M6250) and heated for 10 minutes at 70°C ...
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bioRxiv - Synthetic Biology 2019Quote: Replica-plated cell populations were treated with varying concentrations of 5-aza-2’-deoxycytidine (Sigma-Aldrich, A3656) or Trichostatin A (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... GluR inhibition was performed using 40 µM D(-)-2-amino-5-phosphonopentanoic acid (APV, #A8054, Sigma Aldrich) and 2 µM 6,7-dinitroquinoxaline-2,3-dione (DNQX ...
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bioRxiv - Cell Biology 2021Quote: ... before fixation for 5 hrs in humid conditions at 37 °C in 1.4% / 2% formaldehyde (F8775 Sigma) / acrylamide (A4058 Sigma) ...
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bioRxiv - Neuroscience 2020Quote: All birds received intramuscular injections of 5-Bromo-2’-deoxyuridine (BrdU; 74 μg/g, pH 7.4, Sigma) 3x/day for three days to label mitotically active cells ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked for 2☐h in 5% bovine serum albumin (BSA, Sigma-Aldrich, A7906) in 0.01☐M PBS and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... the cells were spun down and moved to “Phase 2” IMDM media containing 5% human serum (Sigma), 330µg/mL transferrin ...
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bioRxiv - Cancer Biology 2022Quote: ... S-phase populations were identified by incorporation of 10 μM 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 45 min before fixation ...
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bioRxiv - Cell Biology 2022Quote: ... A small piece of frozen BAT (2 – 5 mg) was transferred into a BeadBug tube (Sigma Aldrich) containing 1.0 mm zirconium beads (Merck ...