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Citations for Millipore Sigma :
6951 - 7000 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... although the gel filtration step and second lyophilization were omitted and the temperature needed to elute the protein from Avicel (Sigma-Aldrich) ranged from 50 to 70 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were eluted with phosphate buffered saline containing 300 mM imidazole and 0.1% (v/v) protease inhibitor cocktail (Sigma, P-8849). The proteins were further purified using size-exclusion chromatography ...
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bioRxiv - Systems Biology 2020Quote: ... Urea concentration was subsequently reduced to 2M and proteins were digested overnight at 37°C after the addition of trypsin (Sigma-Aldrich) at a 1:100 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each sample and protein disulphide bonds were reduced with 5 µL of 200 mM Tris(2carboxyethyl) phosphine (TCEP, Sigma-Aldrich) solution and incubated at room temperature for 1.5 hours ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Isotopically labelled proteins were expressed using Escherichia coli BL21 DE3 cells in M9 minimal media supplemented with 15NH4Cl (Sigma-Aldrich ISOTEC). Chaetomium thermophilum Naa5082-289 containing a C-terminal His-tag was expressed using E ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (40 μg) were resolved by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore, MA, USA). Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... the following: 30 µg of each protein (PopB/PcrH, OprF/I, or both) mixed with curdlan (150 µg/dose) (Sigma-Aldrich) and suspended in saline ...
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bioRxiv - Cancer Biology 2022Quote: ... and sDCs were stimulated for 18 h with 1µg/ml of SIINFEKL peptide (OVA 257-264, Proimmune) or with 1mg/ml of low endotoxin soluble ovalbumin protein (sOVA; Sigma-Aldrich) in combination with NaCl or 5 ng/ml of IFNα ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The fractions containing protein were pooled and concentrated to 1 ml final volume using an Amicon Ultra-15 30 kDa concentrator (Millipore, Z717185). This was subjected to gel filtration purification on a Superdex 75 Increase 10/300GL column equilibrated in Equilibration Buffer [50 mM Tris/HCl pH7.5 ...
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bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica, Massachusetts, USA). Huh7.5-SGR cells were seeded in Petri dishes at 106 cells/dish for 24 h prior to transfection with pKSRP-HA plasmids ...
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bioRxiv - Biochemistry 2019Quote: ... and the flow-through containing cleaved protein was collected and concentrated to 2 mL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Genetics 2019Quote: FLAG-tagged proteins were immunoprecipitated from 2 mg of HEK293T or M17 cells using anti-FLAG M2 affinity gel (Sigma A2220). Lysates were prepared in NP40 buffer (see Western blotting) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4 °C with mouse anti-SMI31/32 (to label neurofilament proteins; Millipore NE1022/NE1023; 1:1000) and chicken anti-myelin basic protein (to label myelin ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to a 1 mg/mL protein concentration using 50 mM ammonium bicarbonate and incubated overnight with trypsin (1:200 v/v, Sigma #T6567). Digested samples were acidified and urea removed using a Sep-Pak C18 96-well plate (Waters #186002320) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to a PVDF membrane and incubated with one of the following primary antibodies: α-FLAG polyclonal rabbit (Sigma) or α-RpoA monoclonal mouse (Biolegend) ...
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bioRxiv - Systems Biology 2019Quote: ... The extracted proteins were analyzed by 5-20% gradient SDS-PAGE and transferred onto Immobilon transfer membranes (Millipore, Billerica, MA, USA) for immunoblotting analyses ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Physiology 2020Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Synthetic Biology 2020Quote: ... The loading solution consisted of 15 µL of the protein sample mixed with 1 µL DTT and 15 µL Laemmli 2× Concentrate Loading Buffer (Sigma-Aldrich), and denatured at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on 10% SDS-PAGE gels and electrotransferred onto Immobilon-polyvinylidene difluoride membranes (Millipore Sigma, Burlington, MA). After blocking with 5% non-fat milk ...
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bioRxiv - Immunology 2020Quote: ... 10-15 μg of proteins were loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Molecular Biology 2020Quote: ... The purity of the protein after each step was verified by 12 % SDS-PAGE stained with Coomassie Brilliant Blue R 250 (Sigma-Aldrich). First ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Molecular Biology 2019Quote: ... coli as a fusion protein with the ketosteroid isomerase (KSI) using the pET31 plasmid (Kuliopulos et al., 1994) (Novagen/EMD Biosciences). KSI fusion proteins are concentrated in inclusion bodies in E ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were mixed as indicated in the figure and rotated with either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: Protein samples were obtained by replacing cell culture medium with 1% sodium dodecyl sulfate containing 1X protease inhibitor cocktail (Sigma P8340). Separation was performed in 4-12% Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate containing 200 ug of protein was applied to each Microcon-30 Ultracel PL-30 Regenerated Cellulose 30000 NMWL (Millipore, MRCF0R030) filter unit (1 mg of protein per replicate using 5 filter units in total ...
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bioRxiv - Plant Biology 2020Quote: ... 30 confirmed transgenic plants were screened for dPPRrbcL expression by immunoblot on leaf total protein extracts using HA antibodies (H9658 clone, Sigma-Aldrich) and three lines expressing gradual levels of dPPRrbcL protein were chosen for phenotypic analyses.
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... the thiol groups of the above extracted protein samples from the culture supernatant of 3 groups were alkylated with 50 mM iodoacetamide (cat No. I6125, Sigma, USA) (30 min in dark at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... the protein stock is concentrated using a centrifuge-based filter unit (Ultra-15 Centrifugal Filter Units, Amicon, EMD Millipore, Burlington, MA). The final protein stock concentration is determined by measuring the absorption at 280 nm (Nanodrop ND-1000 ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Cell Biology 2020Quote: ... The EGFP-FLAG-tagged proteins were eluted from the beads with 0.1 mg/mL 3x FLAG peptide (Sigma-Aldrich, Cat. #F4799) in TBS at 4°C for more than 1 hr ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Aliquots (40 μg) of protein samples were subjected to 10% SDS-PAGE and transferred to PVDF membranes (Millipore, Bedford, MA, USA). After blocking with 5% nonfat milk (dissolved in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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bioRxiv - Physiology 2021Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP and Iba-1 protein analyses were generated from previously collected data and normalized to GAPDH (1:5000; cat# G9545, Sigma-Aldrich) [13] ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 8 - 20 mg/mL (Amicon Ultra 10 kDa cut off, Millipore Sigma). Protein samples were stored at 4°C until use.
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
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bioRxiv - Microbiology 2021Quote: The heat extracted proteins from OD600=2 culture was suspended in 1X Laemmli buffer with 250 U of benzonase nuclease (Sigma E0114) and incubated for 5 minutes at 95°C ...