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Citations for Millipore Sigma :
6801 - 6850 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and 20 µg total protein was denatured for 5 min at 70°C in 1X Laemmli Buffer (Sigma Cat # S3401-10VL), resolved on SDS-acrylamide gels and electroblotted to nitrocellulose ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to an Immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The membranes were incubated with primary antibodies overnight at 4⁰C ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged SMOCtail and IFT38 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 mg of total proteins from whole cell extracts were immunoprecipitated overnight at 4°C using GFP-conjugated magnetic beads (Sigma-Aldrich), then beads were washed three times with immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... 1-5ug of antibody was incubated overnight with chromatin and the complex antibody-chromatin was then captured with G protein sepharose beads (Sigma Aldrich) 1h at 4°C ...
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bioRxiv - Genetics 2021Quote: ... purified as previously described18) or HiFi-3xNLS-SpCas9 protein (100 pmol) and sgRNA (300 pmol, IDT) with glycerol (2% of final concentration, Sigma, G2025) and incubating for 15 min at room temperature immediately before electroporation ...
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bioRxiv - Immunology 2021Quote: Egg white immunoglobulins (300 μl) were purified using a commercially available protein A affinity chromatography kit (PURE-1A, Sigma Aldrich Co.). The manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2021Quote: ... the cell lysate proteins were mixed in the indicated combinations and incubated with the ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich) or the Anti-HA Affinity Matrix from rat immunoglobulin G (IgG)1 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Samples were electrophoresed on 7.5% SDS-polyacrylamide gels and proteins were transferred onto the Immobilon-P membrane (Millipore, New Bedford, MA). Blots were probed with anti-fibrinogen polyclonal antibody (1:100,000 dilution) ...
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bioRxiv - Developmental Biology 2022Quote: ... GST and GST-Rab11DN were purified from the supernatant with Glutathione Sepharose 4B GST-tagged protein purification resin (GE17-0756-01, Sigma-Aldrich) using batch method ...
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bioRxiv - Neuroscience 2021Quote: Cortices from P15 Lhx6-Cre;Erbb4+/+;Rpl22HA/HA and Lhx6-Cre;Erbb4F/F;Rpl22HA/HA mice were rapidly dissected in ice-cold RNase-free PBS and immediately homogenized in SynPER Synaptic Protein Extraction Reagent (ThermoScientific) supplemented with 1x cOmplete EDTA-free protease inhibitors (Sigma-Aldrich), 1 mg/ml Heparin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: Cortices from both brain hemispheres were rapidly dissected in ice-cold PBS and immediately homogenized in SynPER Synaptic Protein Extraction Reagent (ThermoScientific) complemented with cOmplete protease inhibitors (Sigma-Aldrich) and phosSTOP phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were electrophoresed on 8% (LRRK2) or 15% (α-synuclein) SDS-PAGE gels and transferred overnight onto PVDF membranes (Millipore). Blots were either blocked with Everyblot blocking reagent (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... 150 μl of lysate from GRloxP/loxP male striata were precleared with 20 μl of protein-A sepharose beads (Sigma, 82506) by a 4 h incubation at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: HPG labeled proteins in supernatant and pellet fractions (∼1 mg) were click-conjugated with 50 µM azide-PEG3-biotin (Sigma-Aldrich) by a Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Following the incubation with a secondary antibody with a linked horse radish peroxidase the proteins were visualized by applying Immobilon™ Chemiluminescent Substrate (Millipore) and detection on an X-ray film.
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bioRxiv - Cell Biology 2022Quote: ... The final Cas9 protein was concentrated to 10 μg/μL using Amicon® Ultra 10 kDa centrifugal filter unit (Millipore UFC801024), aliquoted ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted peak fractions were loaded onto SDS-PAGE to check the proteins and concentrated with Amicon Ultra-4 centrifuge filter (Millipore, UFC3080) and used for next step ...
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bioRxiv - Systems Biology 2019Quote: ... we harvested cells at indicated time points and isolated proteins by lysis in the presence of protease and phosphatase inhibitors (Roth and Sigma-Aldrich), Trichostatin A (APExBio ...
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bioRxiv - Developmental Biology 2019Quote: Somite extract (200μg protein for each condition) was incubated at 37°C for 1h in the presence of 300μM D-NAME (Sigma, Lot#BCBM7105V) or L-NAME (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 nl of 12.5 ng/µl of gRNA and 1 nl of Cas9 protein (300 ng/ul) (Jinek et al., 2012) along with phenol red (Sigma Aldrich) were co-injected at one-cell stage ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Immunology 2019Quote: RNAs were prepared similarly to proteins (collection of paws, slicing) but tissues were homogenized in 2mL of Trizol (TRI-Reagent, Sigma-Aldrich) instead of NP40 lysis buffer ...
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bioRxiv - Synthetic Biology 2019Quote: ... A Bradford colorimetric assay was used to normalize protein concentrations and 35ug lysate in 25uL volume was analyzed in the Luminex Multiplex Assay (EMD Millipore) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2019Quote: ... 10 µL protein G Dynabeads® were washed twice in ChIP dilution buffer and incubated either with 2 µg of mouse IgG (Sigma), anti-H3K27me3 (Abcam ab6002 ...
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bioRxiv - Biophysics 2020Quote: ... and trypsinized at an enzyme to protein mole ratio of 1:50 at 37 °C in a 3M spin-filter device (Millipore Inc.,). Peptides were then desalted using PepClean spin filters (Pierce Inc. ...
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bioRxiv - Genetics 2019Quote: Cell extracts were prepared by TCA precipitation and proteins were separated by 10% SDS-PAGE followed by immunoblotting with anti-HA antibody (Sigma-Aldrich, ref ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were harvested between 24 and 48 h post-transfection and lysed with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology) containing a mammalian protease inhibitor cocktail (SIGMA P8340, Sigma-Aldrich) according to the protocol of the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... and the fluorescence of remaining surface-bound protein was measured using flow cytometry using a Guava EasyCyte 8HT instrument (EMD Millipore). Raw data were processed using FlowJo software (TreeStar Inc.).
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bioRxiv - Biochemistry 2019Quote: ... Protein samples (40 μg/lane) were resolved by 10% SDS-PAGE and transferred to Immobilon-P membranes (Millipore, Billierica, MA, USA) by wet transfer (Trans-Blot ...
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bioRxiv - Neuroscience 2019Quote: ... The protein-DNA complexes of the frontal cortical tissue were crosslinked through incubation with 1% formaldehyde (Sigma-Aldrich, St. Louis, MO) on a rotator for 12 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Beads were washed 5 times in NP40 buffer and precipitated proteins were eluted by boiling with SDS sample loading buffer or elution with 3X FLAG peptide (Sigma-Aldrich). Whole cell lysates and immunoprecipitated samples were analyzed by western blot.
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bioRxiv - Cell Biology 2019Quote: ... The column was washed with KIF3A purification buffer and the protein was eluted in KIF3A purification buffer supplemented with 500 mM imidazole (Sigma#I2399). The eluate was concentrated with a Vivaspin concentrator (Sartorius ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of proteins were further separated using SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Shanghai, China). Membranes were blocked to avoid non-specific bindings in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with primary antibodies for overnight at 4°C and then protein A/G magnetic beads (Millipore, USA) were added for 2 h at 4°C ...
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bioRxiv - Immunology 2019Quote: ... equal amounts of protein obtained from the lysed cells were loaded onto 12% SDS-PAGE gels and transferred onto PVDF membranes (Millipore, USA). After blocking with 5% BSA for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Then cell lysates (500 µg total protein) were incubated with 40 µl of PBS-washed anti-FLAG M2 beads (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: Stable shNT and shGOT1 cells were cultured with or without dox media and protein lysates were collected after five days using RIPA buffer (Sigma, R0278) containing protease inhibitor cocktail (Sigma/Roche ...
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bioRxiv - Biophysics 2019Quote: ... The other was buffer exchanged into SEC buffer using a PD-10 column (GE Healthcare The protein was then treated with 0.4 units of bovine kidney α-L-fucosidase (Sigma) at 18 °C ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore ...
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bioRxiv - Neuroscience 2019Quote: ... After protein transfer blots were blocked in 5% BSA/TBS for 1 h and stained for Sox2 (1:100, Millipore AB5603), Sox9 (1:250 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysate was heat-treated at 70 °C for 5 minutes to denature proteins after which one 20 μl aliquot was mixed with 20 μl Triglyceride Reagent (Sigma T2449) and one 20 μl aliquot was mixed with 20 μl of PBS and incubated at 37 °C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Thirty micrograms (30µg) of total protein from each sample was then reduced with 10mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA) at 55°C for 1h and subsequently alkylated with 18mM IAA (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Thirty micrograms (30µg) of total protein from each sample was then reduced with 10mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA) at 55°C for 1h and subsequently alkylated with 18mM IAA (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Native myoglobin bands were identified by their distinct red-brown colour before general protein staining with EZBlue™ (G104, Sigma-Aldrich). Electrophoretic mobility was assessed on digital gel images and expressed as distance travelled relative to the grey seal myoglobin ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation and target proteins were incubated with 2ml ANTI-FLAG® M2 Affinity gel (A2220, Sigma) at 4°C for 4 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...