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Citations for Millipore Sigma :
6901 - 6950 of 7005 citations for Caspr Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... washed twice in PBS and incubated overnight at 4°C in primary anti-KCC2 raised in rabbit (1:1000, Millipore/Upstate, Cat. #07–432), anti-CGRP raised in mouse (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were pre-blocked with 5% low-fat dry milk in TBS-T and incubated with a rabbit polyclonal anti-KHSRP antibody (Sigma-Aldrich Cat# HPA034739, RRID:AB_10601582), or a monoclonal anti-c-Myc antibody (D84C12 ...
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bioRxiv - Neuroscience 2019Quote: ... PLA was performed using PLA PLUS or MINUS probes for rabbit or mouse anti-serum and Duolink In Situ Detection Reagents Red (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Plant Biology 2019Quote: ... In situ PLA detection was carried out using the appropriate DUOLINK in situ Orange Kit Goat/Rabbit obtained from Sigma-Aldrich (Saint Luis, USA) according to the protocol of the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... Blots were probed with the following antibodies diluted 1:5000 in 1X TBS-T containing 5% nonfat dry milk: rabbit anti-FLAG (Sigma-Aldrich Cat# F7425, RRID:AB_439687), mouse anti-FLAG (Sigma-Aldrich Cat# F1804 ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were washed with 1% BSA/1xPBS prior to addition of Pellets were incubated with 10 µl of mouse anti-rabbit IgG (Sigma RG-96; 1:100) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Western blots were treated with antibodies against the appropriate proteins followed by a second reaction with anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Sigma, St. Louis, MO, USA). The SuperSignal West Pico and Femto substrate kits (Pierce ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 1 hour at 37⁰C with Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma Millipore, Cat. DUO92002) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 1 hour at 37⁰C with Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma Millipore, Cat. DUO92002) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma Millipore ...
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bioRxiv - Microbiology 2021Quote: ... medium (HiMedia Laboratories Pvt Ltd.) was filter-sterilized with 0.2 μm filters and supplemented with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA) for the culturing of B ...
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bioRxiv - Microbiology 2020Quote: ... Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) were received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) was received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Neuroscience 2021Quote: ... One to three 12 μm sections were chosen randomly from both sides for staining as previously established6 using an anti-CGRP antibody (1:5000, Sigma C8189, anti-rabbit polyclonal), an anti-P2X3 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-c-Jun (Thr91, cell signal, 2303, 1:1000), rabbit anti-c-Jun (cell signal, 9165, 1:1000) and mouse anti-GAPDH (Sigma, Cat: G8795, 1:1000). Immunoreactivity was visualized using appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were purchased from the following sources: anti-HA rabbit polyclonal antibody (pAb; #NB600-345, Novus Biologicals, Englewood, CO; #F3165, Millipore-Sigma, St. Louis, MO), anti-DDK (M2 Flag ...
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bioRxiv - Cancer Biology 2022Quote: ... and rabbit anti-pSer502-NT5C2 (dilution 1:1000) (Covance) antibodies and β-actin with a mouse monoclonal anti-β-actin antibody (Sigma Aldrich, #A5441). We visualized the immunoblots using an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Plant Biology 2022Quote: ... and αTubulin levels were used as loading control (α_Tubulin antibody, Monoclonal Anti-α-Tubulin antibody produced in mouse, Sigma, Catalog number T5168; anti-Rabbit IgG, Sigma, Catalog number A0545).
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bioRxiv - Microbiology 2022Quote: ... anti-rabbit Alexa Flour 488 and anti-rabbit Alexa Flour 546 5-(N-ethyl-N-isopropyl) amiloride (EIPA) and chlorpromazine hydrochloride (CPZ) were from Sigma-Aldrich (St. Louis, MO). EIPA was diluted to 10 mM with dimethylsulfoxide ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with a mixture of rabbit anti-P2X7R (1:500) and goat anti-IBA-I (1:1000)/mouse anti-GFAP (1:2000, #G6171, Sigma-Aldrich, St. Louis, MO)/rabbit anti-NeuN (1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then blocked for 3 hours in PBST and 5% normal goat or donkey serum at room temperature after which they were incubated in primary antibodies (rabbit anti-GABA 1:500, Sigma-Aldrich Cat# A2052, RRID:AB_477652) for at least 72 hours at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and rabbit polyclonal anti-β-actin antibody (catalog number A2066, epitope: amino acids 365-375 of human β-actin) were obtained from Sigma (Madrid, Spain). Mouse monoclonal Anti-GOK/STIM1 antibody (Clone 44/GOK ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Sigma-Aldrich, A9044 and A5278), followed by incubation with the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... tissue sections were exposed to anti-Iba1 rabbit antibody (FUJIFILM Wacko, 013-27691, 1:300 dilution) and anti-GFAP mouse antibody (Sigma, G3893, 1:300 dilution) for overnight at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... primary antibody against α-tubulin (T5168) and secondary antibodies (A0545, anti-rabbit; A9044, anti-mouse IgG-peroxidase) were purchased from Sigma-Aldrich (Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore, C2581; Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary fluorescent antibodies were anti-mouse IgG (1:200, Chemicon, AP308F, the USA) conjugated with FITC and FITC anti-rabbit IgG (1:200, Sigma-Aldrich, F1262, the USA). To detect and measure the positive cells ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... Goat anti-rabbit (Alexa fluorTM 488 #A-11034) was added against the primary antibody and mounted with DAPI medium (#F6057, Sigma, St. Louis, MI, USA). Fluorescence was captured in an inverted microscope (Nikon Eclipse TE2000-U).
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST) buffer with 5% bovine serum albumin (BSA) and incubated with primary rabbit anti-β-actin antibody (Millipore Sigma, cat. #SAB5600204) at 1:20000 ...
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bioRxiv - Neuroscience 2023Quote: ... horseradish peroxidase (HRP) conjugated secondary anti-rabbit IgG (cat. A0545) and anti-mouse IgG (cat. A9044) were procured from Sigma-Aldrich (St. Louis, MO). Rabbit polyclonal antibody to Iba1 (cat ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Acetylated histone H4 and H2A were detected by immunoblotting with rabbit anti-acH4 (UBI, Catalog # 06–866, 1:1000 dilution) and anti-acH2A K9 (Millipore Sigma, Catalog # 07-289), respectively.
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies were applied (goat anti-rabbit IgG-Alkaline Phosphatase, A9919, Sigma Aldrich; goat anti-mouse IgG-Alkaline Phosphatase, A4312, Sigma Aldrich; 1:3000) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and then incubated for 1 hour at 37°C with secondary goat anti-rabbit antibodies conjugated with fluorescein isothiocyanate (FITC; 1:250; Sigma-Aldrich, Prague, Czech Republic). Fluorescence intensity was measured using the Synergy H1 microplate reader (BioTek ...
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bioRxiv - Neuroscience 2023Quote: ... then washed three times in PBT and incubated in secondary antibody mix (goat anti-chicken Alexa Fluor 488, Jackson Immunoresearch #103-545-155, 1:200, goat anti-rabbit ATTO 647N, Sigma #40839, 1:100) for 24 - 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS 1X and incubated during 1h at room temperature with Alexa FluorTM 594 donkey anti-rabbit IgG (H+L) (Sigma-Aldrich, cat. nr A21207) secondary antibody 1:500 was used ...
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bioRxiv - Neuroscience 2024Quote: ... Filters were washed three times with TTBS buffer and then incubated for 1 hour with secondary peroxidase-coupled anti-rabbit antibody (1:7000, Millipore, Cat #401393-2 ML). Immunostaining was revealed by enhanced chemiluminescence luminosity (Amersham Pharmacia Biotech ...
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bioRxiv - Neuroscience 2024Quote: ... the first incubation was performed with mouse anti-RFP and rabbit anti-PCP4(respectively: 1:500, Invitrogen, # 710530 and 1:300; Sigma-Aldrich, #HPA005792, RRID: AB_1855086). The secondary incubation was performed with anti-mouse conjugated to Alexa 488 and anti-rabbit IgG2a conjugated to Alexa 568 (respectively ...
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bioRxiv - Cell Biology 2024Quote: ... After overnight incubation the primary antibodies were washed away and PLA secondary probes were added: Anti-Rabbit MINUS (Sigma Aldrich, #DUO92005, dilution 1:5), Anti-Goat PLUS (Sigma Aldrich ...