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Citations for Millipore Sigma :
6751 - 6800 of 7005 citations for Caspr Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with 5% BSA in 0.1% TBS-Tween for 1 hr at room temperature for blocking step and incubated with 1:1000 anti-RFC1 antibody produced in rabbit (Sigma-Aldrich; AV44167) at +4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... was purchased from Enzo Life Sciences and mouse monoclonal anti-β-actin (A5441), along with anti-mouse and anti-rabbit antibodies coupled with HRP (A4416, A6154) were obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA probe anti-mouse PLUS and anti-rabbit MINUS (Duolink®, Sigma-Aldrich). The incubation with all antibodies was accomplished in a humidified chamber for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with primary antibody (15 µg/mL in 3% BSA in PBS): rabbit anti-KCNN4 antibody (IgG, clone HPA053841, Sigma Aldrich®) or control rabbit antibody (IgG ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 38-h incubation in TBS with 0.05% Tween with rabbit polyclonal anti-KCC2 (1:500; EMD Millipore; 07-432) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and FITC-labelled rabbit polyclonal antibody against wheat- germ-agglutinin (WGA) to visualize and quantify the myocyte cross-sectional area (1:100, Sigma Aldrich T4144). Cell surface areas and fibrotic areas were determined using ImageJ imaging software (http://rsb.info.nih.gov/ij/).
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bioRxiv - Developmental Biology 2023Quote: ... and blotting on a polyvinylidene difluoride (PVDF) membrane probed with rabbit monoclonal anti-histone H3 (Cat# 07690, EMD Millipore, 1:25000-50000), mouse monoclonal anti-alpha tubulin (Cat# NB100-690 ...
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bioRxiv - Genetics 2023Quote: ... Duolink probes (mouse plus and rabbit minus) were prepared and applied as described by manufacturer’s instructions for the Duolink® PLA kit Cy5 (Millipore-Sigma DUO92013).
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assays were performed in liver cryosections from frozen tissue using Duolink® In situ Red Starter Kit Mouse/Rabbit from Sigma-Aldrich, according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... sections were stained for ciliated cells (rabbit anti-RFX3, 1:100, Cat# HPA035689,Sigma-Aldrich, and mouse anti-AceTUB, 1:100, Cat# T6793, Sigma-Aldrich), club cells (rabbit anti-SCGB1A1 ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used with indicated dilution into 1×PBS containing 0.5% BSA and 0.1% Triton X-100: rabbit anti-PH3 (Millipore 06-570, 1:1000), mouse anti-FLAG (Sigma F1804 ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used for immunofluorescence were: anti- cleaved Caspase-3 (Asp175) (1:400, rabbit, CST, #9661) and anti-FLAG (1:400, mouse, Sigma-Aldrich, #F3165) for detection of overexpressing proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody solution was prepared with a dilution of 1:100 of both rabbit anti-human anti-ZO-1 (Sigma-Aldrich, AB2272) and mouse anti-human anti-Vimentin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... followed by a two-hour incubation at room temperature in darkness with FITC-coupled α-rabbit antibodies (diluted at 1:200 in BSA-PBS from Sigma Aldrich). After subsequent washing and drying ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with the primary antibodies (anti-aSyn Syn-1 mouse, BD Transduction Laboratories 1:1000; anti-pS129-α-syn Rabbit Abcam Ab51253; mouse anti-β-actin, 1:10.000, Sigma Aldrich) was performed overnight at 4 °C in 5% Albumin Bovine Fraction V (BSA)/TBS-Tween ...
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bioRxiv - Microbiology 2024Quote: ... and subjected to immunoblot analysis using as primary antibodies either the polyclonal produced in rabbits or anti-FLAG® antibody (Millipore Sigma). Rabbit polyclonal antibodies against the S ...
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bioRxiv - Neuroscience 2024Quote: ... sections were treated with 1% H2O2 for 15 min followed by incubation with rabbit-anti-Iba1 antibody (1:1000, FUJIFILM Wako Pure Chemical Corporation, 019-19741) or rabbit-anti-GFAP antibody (1:3000, Sigma-Aldrich, HPA056030) in blocking solution overnight at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with rabbit-anti-Iba-1 primary antibody (1:1000, Wako Chemicals; Cat. No) and WFA (1:400; Sigma; Cat. No) for 23 hours at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated in secondary antibody (Alexa Fluor 488 Goat anti-Rabbit, 1:500, Invitrogen #11008; DAPI, 1:1200, Sigma- Aldrich #D9564) at 4°C in the dark overnight with rocking ...
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bioRxiv - Cell Biology 2019Quote: ... SAF-A:RNA complexes were detected in BrU-labeled cell populations using the rabbit α-SAF-A and mouse α-BrU antibodies in conjunction with the Duolink Proximity Ligation Assay kit (Sigma, DUO92102).
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bioRxiv - Cell Biology 2020Quote: ... Western blots were probed using the following primary and secondary antibodies (incubated in PBS, 0.1% Tween20 and 5% milk): rabbit anti-Eps8L2 (Sigma-Aldrich #HPA041143, 1:500), mouse anti-Flag (Sigma-Aldrich #F1804 ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were incubated overnight at 4°C in primary antibody diluted in 5% BSA/PBS using the following concentrations: 1:500 rabbit-α- PfACP48 (kind gift from S. Prigge) and 1:100 rat-α-HA 3F10 (Sigma 11867423001). Coverslips were washed 3 times in PBS ...
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bioRxiv - Genetics 2021Quote: ... Brains were then incubated with secondary antibody (goat anti-mouse Alexa 488 or 555, or goat anti-rabbit Alexa 488 or 555, Sigma Aldrich, 1:500) and mounted with Antifade ...
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bioRxiv - Plant Biology 2021Quote: ... and subjected to a one-step immuno-affinity reaction using the rabbit peroxidase-anti-peroxidase soluble complex antibody (Sigma-Aldrich, St. Louis, USA). The membrane was developed ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Neuroscience 2021Quote: ... then blocked with 5% normal goat serum in 0.5% PBS-T for 2 hr at RT and stained with pre-absorbed rabbit anti-PER (1:1000) 50 and mouse anti-GFP (Sigma G6519, 1:200) in 5% goat serum in 0.5% PBST for at least 48 hr at 4°C ...
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bioRxiv - Zoology 2019Quote: ... then washed in PBS with 0,1% Triton X100 and incubated in the secondary anti-rabbit CF488 antibody (SAB4600044, Sigma-Aldrich, diluted 1:1000) for 8 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated overnight with primary antibodies (rabbit phospho-AURKA Thr288, 1:50; mouse anti-γ-tubulin GTU-88, Sigma-Aldrich; 1:1000) diluted in blocking buffer at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were the following: rabbit polyclonal anti-ERGIC-53/p58 and mouse anti-MAP2A purchased from Sigma-Aldrich (St. Louis, MO, USA) (code ...
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bioRxiv - Bioengineering 2020Quote: The following primary antibodies were incubated for 1 hour at room temperature or for 12-18 hours at 4°C for the following markers: rabbit PCM-1 (1:100 dilution, Sigma-Aldrich, HPA023370-100UL), mouse PCM-1 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... Live-labelling of surface CNIH was performed at 48 hours post-transfection by incubation with rabbit anti-HA (Sigma, Cat# H6908; 1:200) for 20 min at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies and dilutions used in this study were: mouse anti-PV (1/4000; Swant, Marly, Switzerland) and rabbit anti-GABA (1/200; Sigma-Aldrich, Dorset, UK). Upon completion of the primary incubation step ...
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bioRxiv - Microbiology 2021Quote: ... The blot was washed with TBST (4x 5 min) and incubated with a goat anti-rabbit alkaline phosphatase secondary antibody (Sigma-Aldrich, Steinheim, Germany) diluted 1:30,000 in TBST containing 3 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Microbiology 2020Quote: ... The anti-Psa antibodies (see below) were used at 1:5,000 dilution and the Anti-Rabbit IgG-Peroxidase antibody (Sigma-Aldrich, St Louis, MO) was used at 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: In situ Proximity Ligation Assay (PLA) was performed with Duolink in situ Red Starter kit Mouse/Rabbit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate, EMD Millipore – AP160P; Rabbit IgG-HRP linked, EMD Millipore – 12–348) at room temperature for 1 hr and visualized with Immobilon Western chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Physiology 2022Quote: ... and with peroxidase- conjugated secondary antibodies (Anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P; Anti-rabbit IgG, HRP-linked, EMD Millipore, 12-348) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-FLAG rabbit polyclonal and mouse monoclonal (Cat# F7425, RRID: AB_439687 and Cat# F1804, RRID: AB_262044, respectively, Sigma-Aldrich, St. Louis, MO, USA), anti-GFP rabbit polyclonal (Cat# A-11122 ...
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bioRxiv - Genetics 2022Quote: ... in PBS and incubated with rabbit polyclonal vault antibodies (N2-B15, provided by Leonard Rome, dilution 1:1000) and mouse anti-NeuN antibodies (MAB377, Millipore, dilution 1:1000) in 0.1%NDS/PBS at 4 °C overnight under agitation ...