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Citations for Millipore Sigma :
6701 - 6750 of 7005 citations for Caspr Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink® In Situ PLA Probe Anti-Rabbit MINUS and Anti-Mouse PLUS and Duolink® In Situ Detection Reagents (Sigma-Aldrich) were used ...
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bioRxiv - Neuroscience 2020Quote: Immunostaining for ChAT or TH was performed using the following antibodies: rabbit anti-TH 1:200 (Pel-Freez cat # P4010-0) or goat anti-ChAT 1:25 (Millipore cat # AB144P). Sections were fixed in an acetone/methanol mixture (1:1 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies used were rabbit polyclonal against Arl13b (kindly provided by Dr. Tamara Caspary, Emory University) and a mouse monoclonal against acetylated α-tubulin (Sigma-Aldrich). Cells were stained with secondary antibodies along with DAPI diluted in dilution buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... Antibody solution consisted of blocking solution with rabbit anti-Iba1-635 (Wako, cat. # 013-26471) at 1:400 and DAPI (Sigma, cat. # D9542) at 10 µg/mL.
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bioRxiv - Microbiology 2021Quote: ... (80) and α-PilC (1:5000 dilution) (81) antibodies were used together with horseradish-conjugated goat anti-rabbit immunoglobulin G (Sigma-Aldrich) or anti-mouse sheep IgG antibody (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight followed by goat anti-rabbit secondary antibody conjugated with Cy3 for 1 hr at room temperature (1:500, Millipore, Cat. #AP132C). Since the purpose of this staining was to evaluate the presence of extracellular HMGB1 in the tendon ...
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bioRxiv - Cell Biology 2021Quote: ... tissue sections were washed 3 times with PBS and incubated at room temperature for 2 hrs with Cy3-conjugated goat anti-rabbit IgG antibody (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Genetics 2020Quote: ... using 1:1000 rabbit anti-Bestrophin1 antibody (LAgen Laboratories; Cat# 016-Best1-01) and 1:1000 mouse anti-Actin antibody (Millipore; Cat# MAB1501) as primary antibodies.
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated in PBS-T-milk for 1 h using 1:10000 dilutions of anti-rabbit or anti-mouse HRP-linked secondary antibodies (A0545 or A9044 Sigma antibodies, respectively) as required ...
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Neuroscience 2022Quote: ... We combined this immunohistochemistry with the one for serotonin (5-HT) using the rabbit IgG anti-5-HT (1/500, Sigma-Aldrich, 49545) and the goat anti-rabbit Fluor Alexa 568 (1/500 ...
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bioRxiv - Neuroscience 2022Quote: ... a second set of tissue from each rat was incubated in a cocktail of rabbit anti-cFos (1:1000) and a mouse monoclonal antiserum against dopamine beta-hydroxylase (DbH; 1:5000; Millipore, MAB308; AB_2245740), followed by a cocktail of Cy3-conjugated donkey anti-rabbit IgG (red ...
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit anti-TgTom40 (van Dooren et al., 2016, 1:2000 dilution) and rat anti-HA high affinity (Sigma, 11867431001, 1:200 dilution) primary antibodies were used ...
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bioRxiv - Neuroscience 2023Quote: ... and stained according to the immunohistochemistry protocol above (primary antibodies: anti-ZnT3, rabbit polyclonal, 1:500, Synaptic Systems; anti-puromycin 1:1000, EMD Millipore) and imaged on the Zeiss LSM 880 with Airyscan using a LD LCI Plan-Apochromat 25x/0.8 mm Korr DIC M27 and 1.8x zoom ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the coverslips were washed three times in PBS and incubated with donkey anti-rabbit CF 647 (Sigma, SAB4600177, 1:2000 dilution) and goat anti-rat AlexaFluor 488 (Thermo Fisher Scientific ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the slide was washed three times with the wash buffer and stained sequentially with the Immunol Fluorence Staining Kit with Alexa Fluor 647-Labeled Goat Anti-Rabbit IgG (P0180, Beyotime, China) and 4’,6-diamidino-2-phenylindole (DAPI, MBD0015, Sigma-Aldrich, USA) as per the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-myosin 7a (Myo7a; 1:100, Proteus Bioscience, PAB 25–6790) or Phalloidin Alexa Fluor 647(1:100, Millipore Sigma A22287). A 40X z-series with 1-μm steps were collected with a confocal microscope (Stellaris ...
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bioRxiv - Molecular Biology 2024Quote: ... duttoni (NIH Rocky Mountain Laboratories, Hamilton, MT) were grown in BSK-H medium with 6% rabbit serum (Sigma Aldrich, St. Louis, MO) until the culture reached log phase (∼4 days) ...
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bioRxiv - Neuroscience 2024Quote: ... The primary and secondary antibodies used for GABA-IHC were a rabbit anti-GABA antibody (1:200; Sigma Aldrich, Burlington, MA; RRID:AB_477652) and an Alexa Fluor® 647 AffiniPure™ Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Genetics 2024Quote: ... and secondary antibodies (anti-mouse HRP [1:2,000; Thermo Fisher Scientific] for TARS1 and anti-rabbit HRP [1:5,000; EMD Millipore] for actin) were applied in 2% milk solution ...
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bioRxiv - Genetics 2023Quote: ... Duolink probes (mouse plus and rabbit minus) were prepared and applied as described by manufacturer’s instructions for the Duolink® PLA kit Cy5 (Millipore-Sigma DUO92013).
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bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assays were performed in liver cryosections from frozen tissue using Duolink® In situ Red Starter Kit Mouse/Rabbit from Sigma-Aldrich, according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... sections were stained for ciliated cells (rabbit anti-RFX3, 1:100, Cat# HPA035689,Sigma-Aldrich, and mouse anti-AceTUB, 1:100, Cat# T6793, Sigma-Aldrich), club cells (rabbit anti-SCGB1A1 ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed again and incubated for 1 hour min with secondary FITC-conjugated goat anti-rabbit antibody (Sigma-Aldrich; 1:100) and TRITC-conjugated phalloidin ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were diluted in 10% goat serum and incubated for 18 hours at 4°C with laminin rabbit anti-mouse antibody (1:250, Sigma-Aldrich, L9393). Sections were then incubated for 1 hour at room temperature in their corresponding goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: AKT:FBP1:ALDOB:PP2A-C interactions in liver tissue were detected with an in situ PLA kit (Duolink® In Situ Red Starter Kit Mouse/Rabbit, Sigma, DUO92101) (Su et al. ...
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bioRxiv - Microbiology 2023Quote: ... the treated cells were performed using anti-chicken IgY (IgG) (whole molecule)-FITC antibody produced in rabbit (Sigma–Aldrich, dilution 1:640) as the secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were purchased: rabbit anti-PITPNB polyclonal antibody (pAb) and sheep anti-TGN46 pAb from Sigma-Aldrich (St. Louis, MO); rabbit anti-β-tubulin pAb from cell signaling (Danvers ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 38-h incubation in TBS with 0.05% Tween with rabbit polyclonal anti-KCC2 (1:500; EMD Millipore; 07-432) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: The cardiac sections were processed via the indirect immunofluorescence method using rabbit polyclonal antibodies against tyrosine hydroxylase (TH, Millipore, #AB152; 1:100) to stain adrenergic nerve fibers and growth association protein 43 (GAP-43 ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 µl wash buffer containing 10 µl 1 µg/µl anti-rabbit secondary antibody raised in guinea pig (Sigma-Aldrich, SAB3700889) and 1 U/µl RNase inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... the first incubation was performed with mouse IgG2a anti-RGS14 (1:50, UC Davis/NIH NeuroMab Facility, #73-170) and rabbit anti-PCP4 (1:400, Sigma-Aldrich; #HPA005792). The secondary incubation was performed with anti-mouse IgG2a or anti-rabbit conjugated to Alexa 488 (ThermoFisher ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-VMAT2 (1:2000, gift of Dr. Gary Miller, Colombia University) and mouse anti-MAP2 (1:2000, Millipore Cat# MAB3418, RRID:AB_94856). These were subsequently detected using goat anti mouse or rabbit Alexa Fluor-488-conjugated (A-11001 RRID:AB_2534069 ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated for 48-72 hours with the following primary antibodies at 1:400: rabbit anti-Iba1 (Wako, 019-19741) and mouse anti-PSD-95 (Sigma-Aldrich, MAB1598). Sections were then washed 3x with PBS containing 0.05% Tween 20 and incubated with Alexa Fluorophore conjugated goat anti-rabbit IgG (H+L ...