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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
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bioRxiv - Molecular Biology 2023Quote: The cells were then incubated at 37°C for 4 h in the presence of 25 μM 5-iodo-2′-deoxyuridine (IdU; Sigma-Aldrich, St. Louis, MO). After washing cells with PBS ...
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bioRxiv - Plant Biology 2023Quote: ... and SIX6 with an internal TEV protease cleavage site for pro-domain removal were processed with 2 to 4 units of thrombin from bovine plasma (600-2,000 NIH units/mg protein) (Sigma-Aldrich Inc., St. Louis, USA) per mg of protein at 4°C or TEV protease (produced in-house ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma) for at least 1 day at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
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bioRxiv - Plant Biology 2020Quote: Spin trapping assays with the spin probe 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN; Sigma-Aldrich, St. Louis, USA) to detect the formation of hydroxyl radicals24 were carried out using cyanobacterial cells (OD730 = 0.65 ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged (500g at 4°C for 4 minutes) and resuspended in PBS containing 1 μg/mL DAPI (Sigma Aldrich, D9542-100MG). Analysis of flow cytometric data was preformed using the BD Fortessa X20 and FlowJo v10 software (Ashland) ...
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed for 1 day in 4% PFA at 4°C before transferring to 30% sucrose (Millipore Sigma) in PBS at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... they were incubated in the primary antibody solution composed of anti-aquaporin 4 (AQP-4) (1:300 dilution in blocking buffer, Millipore Sigma) and anti-LRRC8A antibody (1:200 dilution in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Bioengineering 2022Quote: Specimens were permeabilized with 4% paraformaldehyde and incubated for 1 hour at room temperature in primary antibody anti-vinculin (1:500, Sigma Aldrich, V4505), secondary antibody Alexa Fluor 488 (1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were incubated overnight at 4°C with primary antibodies, GFP (Novus NB1001770, 1:100) and NeuN (EMD Millipore MAB377, 1:150) in 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162; PAX6 mouse 1:80, DSHB) diluted in solution containing 2.5% NGS and 0.02% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were fixed with 4% PFA and immunostained with mouse anti-HA (Covance, 1:1000) or rabbit anti-Flag (Sigma, 1:1000), followed by incubation with AlexFluor-647-conjugated anti-human Fc γ and species-specific AlexaFluor-488- or -546-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1 h at RT and after washing coverslips were incubated for 4 min with DAPI (1:4000, Sigma-Aldrich #D8417) and mounted with ProLong Gold Antifade reagent (Invitrogen #P10144).
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bioRxiv - Cell Biology 2023Quote: ... and applied on previously seeded NIH3T3 Flp-In cells at a ratio of 1:1 (vol/vol, supernatant : NIH3T3 growth medium) together with 4 µg/ml Polybrene® (Sigma-Aldrich). Transduced cells were cultured in regular growth medium supplemented with 5 µg/ml blasticidin (Carl Roth GmbH + Co ...
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bioRxiv - Cell Biology 2023Quote: ... cells were harvested on ice and lysed by rotation for 1 h at 4°C in JIES buffer (see above) containing 1× SIGMAFAST protease inhibitor cocktail (Sigma-Aldrich, S8830). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in TBS-T with 5 % non – fat milk for 1 hour in RT following incubation overnight at 4 °C with primary (1:1000) antibodies: Flag (Sigma, Cat. F1804), IL-1β (Cell Signaling Technology ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... and overnight at 4 °C with primary antibody against Nucleolin or NPM1 (Nucleolin - abcam, ab22758 1:1000; NPM1 – Sigma, B0556 1:500). Cells were washed three times with 1X PBT for 5 minutes each and then incubated for 1 h at room temperature with secondary antibody (goat anti-rabbit IgG (H+L ...
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bioRxiv - Genetics 2024Quote: ... in TBS-T at room temperature for 1 h and incubated at 4 °C overnight with mouse-anti FLAG (1:400, Sigma-Aldrich, F3165) or mouse-anti Cas9 (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... isolated brains were post-fixed in 2% PFA overnight and embedded in 2% low-melt agarose (Sigma-Aldrich, St. Louis, MO, USA) in DPBS ...
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bioRxiv - Microbiology 2021Quote: ... Three subsamples (∼2 ml) were taken from each section with a 3 ml syringe and immediately fixed in 2 ml RNAlater (Sigma Life Science) in a 5 ml tube (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... The following chemicals were prepared and used according to the manufacturers’ guidelines: 2-deoxy-D-glucose (2-DG, Sigma, cat. no. D8375), Na-iodoacetate (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... the solutions were replaced by the fixing solution overnight (2% glutaraldehyde, 2% paraformaldehyde [EM grade; Science Service GmbH, Germany] in 100 mM aqueous sodium cacodylate buffer [Sigma Aldrich, Germany]). Without rinsing the cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stock solutions of fluorescent protein (20 mg/mL and 0.2 mg/mL) and total RNA from torula yeast type VI (2 mg/mL and 0.02 mg/mL) (Sigma-Aldrich #R6625-25G) were prepared in 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... sonicated for 2 minutes of 2 sec on/8 seconds off cycles on ice using probe sonicator (Ultrasonic processor 500W, Sigma Aldrich, USA). 3-5 mL of 5 % NaCl was added to the samples and after thorough vortexing the phases were separated by centrifugation at 3,000 × g for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: Tumors excised from AKB6 xenografts that were treated with vehicle (n=2) and MRTX1133 (n=2) were digested using Liberase (Sigma Cat # 05401020001) to get single cells and sent to RPCI’s Genomics Shared Resources for sequencing using 10X Chromium and Illumina’s NovaSeq instruments ...
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bioRxiv - Physiology 2022Quote: ... the mice were deeply anesthetized with isoflurane and the brains were sectioned into 2-mm-thick sections and placed in 2% 2,3,5 triphenyltetrazolium chloride (TTC, cat. #T8877, Sigma-Aldrich, St. Louis, MO) for 10 min at 37 °C to delineate infarcts ...
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bioRxiv - Plant Biology 2024Quote: ... specific primers for each T-DNA line (Supplemental Table 2) were designed using SALK site (http://signal.salk.edu/tdnaprimers.2.html) and were ordered from Sigma (https://www.sigmaaldrich.com/GB/en).Ten seeds from each T-DNA mutant line were sown and seedlings were picked and transferred to pots ...
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bioRxiv - Microbiology 2024Quote: ... the maintenance and expansion of T cells was done by adding medium with 10 ng/ml interleukin-2 (IL-2, Stemcell) (10 ng/ml) and for activation Lectin from Phaseolus vulgaris (red kidney bean, PHA) (Sigma, 1µg/ml).
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bioRxiv - Biochemistry 2024Quote: ... Catalog No: ASC1746) + 2 μL Tris-(2-carboxyethyl)-phosphine (TCEP) (50 mM, freshly prepared in MilliQ water) (Sigma; Catalog No: C4706-2G) + 1 μL rhodamine-azide (10 mM in DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... Pregnant females received double intraperitoneal (IP) injections (separated by 2 hours) of 50 mg/kg of 5-Iodo-2′-deoxyuridine (IdU; Sigma Aldrich, I7125) followed by 50 mg/kg 5-Chloro-2′-deoxyuridine (CldU ...
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bioRxiv - Neuroscience 2024Quote: ... endogenous mouse microglia depletion was achieved by 2 consecutive (P2 and P3) intraperitoneal injection of the CSF1R inhibitor BLZ945 (dissolved in 20% (2-hydroxypropyl)-β-cyclodextrin (Sigma-Aldrich) at a dose of 200mg kg−1 body weight37 ...
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bioRxiv - Cell Biology 2024Quote: ... Male and female tumor-bearing mice were treated for 14 days by supplying acidified vehicle drinking water (HCl, pH4) consisting of 2% w/v (2-Hydroxypropyl)-β-cyclodextrin and 10% w/v sucrose (both Sigma-Aldrich) or vehicle water containing 60 μg/ml buparlisib ...
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... For visualization of the eya1 probe the samples were incubated with an anti-Dig-POD antibody (1/50 in 2% TNB, Sigma-Aldrich REF 11 207 733 910) and DAPI (1/1000 ...
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bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
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bioRxiv - Plant Biology 2021Quote: The dried samples were derivatized for 2 hours at 37 °C in 50 μI of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
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Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerancebioRxiv - Plant Biology 2022Quote: ... 150 mg/L cefotaxime] containing 20 mg/L hygromycin B and incubated at 26 °C under conditions of 16-h light (150 μmol photons m−2 s−1) and 8-h dark until roots were established and leaves touched the Phytatray™ II lid (Sigma-Aldrich, St. Louis, MO, USA). Plantlets were then transferred to soil.
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bioRxiv - Genomics 2023Quote: ... undifferentiated iPSC colonies were treated with 5 μM of the Y27632 compound and dissociated to single cells with Accutase (Millipore, 1:2 dilution in PBS 1X). Four million dissociated cells were seeded in each well of a 6-well plate and cultured in mTeRS1 with 10 μM Y27632 compound on an orbital shaker at a speed of 95 rpm ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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IL-6 SIGNALING EXACERBATES HALLMARKS OF CHRONIC TENDON DISEASE BY STIMULATING REPARATIVE FIBROBLASTSbioRxiv - Bioengineering 2024Quote: ... the assembloids were removed from the clamps and washed with PBS before staining them with Ethidium Homodimer (EthD-1, Sigma-Aldrich, 2 mM stock in DMSO) diluted to 4 µM with PBS (20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured as described in 39 using the following procedure: Cord blood samples were diluted 1:2 in PBS and layered on Ficoll® Paque Plus (Sigma-Aldrich by Merck, Darmstadt, Germany) to isolate mononuclear cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were incubated overnight at 4°C with anti-MCU (1:50, Sigma-Aldrich), anti-Ki67 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... and at DIV10 we treated hippocampal neurons with 1 µM 4-OH-tamoxifen (Sigma) to induce partial Ift88 deletion ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices were placed in 1 × PBS containing 4% (w/v) acrylamide (Sigma-Aldrich) and 0.25% (w/v ...