Labshake search
Citations for Millipore Sigma :
6801 - 6850 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... We eluted the bound proteins from the affinity beads with 2× SDS-PAGE sample buffer and analyzed the eluates by immunoblotting with anti-GFP (SAB4301138, Sigma-Aldrich, USA, 1:1,000 dilution), anti-FLAG (F1804 ...
-
bioRxiv - Plant Biology 2024Quote: ... Tissue was ground in ice-cold protein extraction buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich], 1% protease inhibitor cocktail [Sigma-Aldrich], 2 mM 2,2’-dipyridal disulfide) with a chilled mortar and pestle ...
-
bioRxiv - Immunology 2024Quote: ... at 37°C in digestion medium (RPMI supplemented with 2% iHS [Sigma], 1 mg/ml Collagenase XI [Sigma], and 10 U/ml DNase I [Sigma]). Following digestion ...
-
bioRxiv - Biochemistry 2022Quote: ... and mixed with 100 μL of 2× GPCR solution ligand (2 μM neurotensin for FLAG-NTSR1 or 20 μM Isoproterenol (Sigma-Aldrich) for FLAG-β2AR) ...
-
bioRxiv - Molecular Biology 2021Quote: J1 mES cells with doxycycline-inducible short hairpin RNA-micro RNA (shRNA – Supplementary Table 2) sequences targeting Tet1 or Tet3 were treated for 48h with 2 μg/mL doxycycline (D9891, Sigma Aldrich).
-
bioRxiv - Immunology 2022Quote: PBMC or expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptides (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice with PBS1X (5 mM EDTA, 2% fetal bovine serum (FBS)) before being fixed in 2% formaldehyde solution (252549, Sigma-Aldrich). After incubation at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Cysteine thiols were subsequently reduced and alkylated by adding Tris(2-carboxyethyl)phospin (Carl Roth) to a final concentration of 10 mM and 2-Chloroacetamide (Sigma-Aldrich) to a final concentration of 40 mM ...
-
bioRxiv - Biophysics 2021Quote: ... Cardiomyocytes were attached to imaging chambers (laminin-coated coverslips attached to acrylic slides) for 2 hours at 37°C and fixed in 2% paraformaldehyde (Sigma-Aldrich) (w/v ...
-
bioRxiv - Neuroscience 2020Quote: ... Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich) and 4% BSA (Bovine Serum Albumin ...
-
bioRxiv - Plant Biology 2020Quote: Stock solutions of 60 mM PAO (Phenylarsine oxide) in DMSO and 50 mM 2-BP (2-bromopalmitate) in EtOH were ordered from Sigma-Aldrich. Seven-day-old seedling grown in vitro vertically (root tip analyses) ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... PLGA-PEI NPs were dispersed in 100 mM phosphate buffer with EDTA (2 mM) (pH = 8.0) and mixed with excess amount of 2-iminothiolane (Traut’s reagent, Sigma-Aldrich, Cat# I6256). After continuous rotation overnight at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... participants were administered a dosage of 2 g tyrosine (supplied by The Hut.com Ltd.) or placebo (2 g cellulose powder (Sigma-Aldrich Co.), solved in 200 ml orange juice (Solevita ...
-
bioRxiv - Neuroscience 2020Quote: ... the mice were anesthetized with 20 mg/kg 1.25% Avertin (a mixture of 12.5 mg/mL of 2,2,2-Tribromoethanol and 25 μL/mL 2-Methyl-2-butanol, Sigma, St. Louis, MO, USA) through intraperitoneal injection and then placed in a prone position in the stereotaxic frame ...
-
bioRxiv - Immunology 2020Quote: ... Cells are washed in an excess of HBSS + 2%FBS and are resuspended in HBSS + 2%FBS with DAPI (Sigma-Aldrich) to discriminate live from dead cells.
-
bioRxiv - Neuroscience 2022Quote: ... 1:400) for 2-h at room temperature and then stained with 4,6-diamidino-2-phenylindole dihydrochloride (DAPI, #D9542, Sigma, Darmstadt, Germany) for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... The total volume was concentrated to ∼2 ml by spin filtration using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane (Sigma-Aldrich). The non-vesicular protein fraction volumes were each collected in 2 ml of PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated with frequent agitation at 37°C for 20 min in 2 ml of 2 mg/ml papain (Sigma, P4762) solution in Hibernate-A ...
-
bioRxiv - Immunology 2020Quote: ... in 96 U-bottom well plates in the presence and absence of 50 mM 2-Deoxy-D-glucose (2-DG) (Sigma-Aldrich). To stimulate them ...
-
bioRxiv - Immunology 2020Quote: ... Cryptococcus was grown for 18 hours overnight in 2 ml yeast peptone dextrose (YPD 50 g/L Fisher, 2% agar Sigma-Aldrich) medium ...
-
bioRxiv - Cell Biology 2021Quote: ... LECs and imLECs were starved in serum starvation medium (EBM-2 with hydrocortisone, ascorbic acid, GA-1000 all at proprietary concentrations and 2% bovine serum albumin (BSA, Sigma-Aldrich)) for 24 h ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4° C with the following primary antibodies in 1X PBS containing 2% Triton X-100 and 2% horse serum: mouse anti-NeuN (EMD Millipore), rat anti-Ki-67 (Invitrogen) ...
-
bioRxiv - Cell Biology 2020Quote: ... cultured for 24 hr in regular ES cell media followed by 2 days of selection using 2 μg/ml puromycin (Puro) (Sigma-Aldrich) and collected for subsequent analyses ...
-
bioRxiv - Immunology 2020Quote: Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... 300 mM NaCl, 0.5% Nonidet P-40, 2 mM EDTA, 2 mM MgCl2, 0.5 M urea and protease inhibitors; Sigma; cat# P-8340) followed by centrifugation at 4°C for 20 min at 16,100 × g ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteolysis was stopped by mixing equal volumes of lysis buffer supplemented with 2 mM PMSF and 2 μg/mL CLAP (chymostatin, leupeptin, antipain and pepstatin (Sigma-Aldrich)) with the lysates ...
-
bioRxiv - Neuroscience 2023Quote: ... BP151-500) in 1X PBS then blocked for 2 hours at RT with 2% bovine serum albumin (BSA, Sigma-Aldrich, A2153), 0.1% Triton in 1X PBS ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Albumin and Lipocalin-2/NGAL values were obtained using MILLIPLEX MAP Rat Kidney Toxicity Magnetic Bead Panel 2 (Millipore Sigma, USA) with 1:500 dilution ...
-
bioRxiv - Bioengineering 2023Quote: ... 2.71 g of CaO (calcined Ca(OH)2 (Jost Chemical Co., USA)) and 0.438 g of Sr(NO3)2 (Sigma-Aldrich, Germany) are mixed in deionized water (300 mL) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with PBS + 2% FBS and then resuspended in 400 μL PBS + 2% FBS containing DAPI (Millipore Sigma, D9542 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and primary cells of BC tissues were concentrated in six-well plates coated with 2% poly-2-hydroxyethyl methacrylate (Sigma-Aldrich) at a density of 1×104 cells/ml in serum-free DMEM/F12 (Gibco ...
-
bioRxiv - Immunology 2023Quote: Yp was cultured to stationary phase at 28°C and 250 rpm shaking for 16 hours in 2×YT broth supplemented with 2 μg/ml triclosan (Millipore Sigma). Mice were fasted for 16 hours and subsequently inoculated by oral gavage with 200 μl phosphate-buffered saline (PBS ...
-
bioRxiv - Immunology 2023Quote: ... cells were stained in PBS + 0.5% BSA + 0.1% sodium azide + 2% Normal Mouse Serum (NMS) and 2% FBS (Sigma, St. Louis, MO). Rat anti-mouse purified CXCR5 (2G8 ...
-
bioRxiv - Neuroscience 2023Quote: ... coverslips containing cultured DRG neurons were transferred to a new 35mm dish containing 5µM Fura-2- acetoxymethyl ester (Fura-2 AM) (Millipore Sigma 344905) in complete neurobasal media and placed in a 37°C + 0.5% CO2 incubator for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... Where indicated, glucose-free cultures were hexose-supplemented using D-glucose (2 g/L) (Fisher, D-galactose (2 g/L) (Sigma-Aldrich), or D-mannose (125 µM ...
-
bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma], and 0.1% Tween-20 [P9416; Sigma] in PBS) for 45 min ...
-
bioRxiv - Immunology 2024Quote: ... 500,000 cells were incubated for 15 min with 7-AAD (200 μl) in PBS supplemented with 2% FCS and 2 mM (ethylenedinitrilo) tetraacetic acid (EDTA) (Sigma-Aldrich), at 4 °C in the dark ...
-
bioRxiv - Neuroscience 2024Quote: ... 500 µg of protein was incubated with 2 µg of either rabbit polyclonal Anti-TARP γ-2 (Millipore Sigma-Aldrich, #AB9876), rabbit polyclonal Anti-TARP γ-3 (Synaptic Systems ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2-Deoxy-D-glucose (2DG) stock solution (2 M) was diluted to a final concentration of 150 mM (Sigma D8375-5G), oligomycin stock solution (10 mM ...
-
bioRxiv - Molecular Biology 2024Quote: ... the cells were washed 2-3 times with warmed PBS followed by 50 μM 5-chloro-2’-deoxyuridine (CldU, Sigma #C6891) for 20 minutes or 50 μM CldU with 4mM hydroxyurea (HU ...
-
bioRxiv - Pathology 2024Quote: ... the deparaffinized and rehydrated sections were stained in 2% Hydrochloric Acid (Huber Lab, Aesch, Switzerland) and 2% Potassium Ferrocyanide (Sigma-Aldrich) (1:1) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Six-well plates can be pre-coated with 2-hydroxyethyl methacrylate solution (1.2 g of 2-hydroxyethyl methacrylate (Sigma-Aldrich, P3932) in 50 ml of 95% ethanol ...
-
bioRxiv - Developmental Biology 2024Quote: ... and this 2 µl volume was then applied to a mixture of 6 µL HBSS and 2 µL thrombin (Sigma, T7513). Ca2+ imaging was performed on an LSM780 in an open bath chamber (JG-23 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The purification involved the chemical cross-linking of purified antigens to N-hydroxy succinimidyl-Sepharose beads (Sigma). The elution process was carried out under low pH conditions ...
-
bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane pellets were solubilized in 4 mL TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH = 7.4) with 2% Triton X-100 (Sigma-Aldrich, St Louis, MO, USA). The solubilized membrane-fraction was loaded on a column containing 1 mL anti-DYKDDDDK (Flag ...
-
bioRxiv - Immunology 2023Quote: ... then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat. No. S1888) in PBS for 48 hours at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...