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Citations for Millipore Sigma :
6751 - 6800 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... 500µg of mitochondria were progressively solubilized 1-4% digitonin (Sigma-Aldrich #D141-500MG) dissolved in water ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM dithiothreitol (DTT) and 4 μg/mL DNase (Sigma catalog no. DN25). Cells were lysed using a French press and the lysate was centrifuged at 30,000 × g for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (34 μM, Sigma), copper (II ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-detyrosinated (glutamated) tubulin (1:1500 overnight at 4°C) (#AB3201, Millipore), anti-rabbit- IgG-HRP (1:7500 ...
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bioRxiv - Bioengineering 2023Quote: ... was coated with a 4:1:5 v/v/v collagen IV (Sigma) / fibronectin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: T-NMOs were fixed for 1 hour in 4% paraformaldehyde (PFA, Sigma-Aldrich) and NMOs for 1 hour in 2% PFA ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature and incubated with Hoechst 1:2000 (Sigma # 23491-45-4) prior to analysis with ImageStream (Flow Cytometry PCMM core facility at BCH and Harvard Medical School).
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bioRxiv - Microbiology 2024Quote: ... Proteins in the supernatant were precipitated with 1:4 trichloroacetic acid (Sigma Aldrich). The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies used for IHC were against ING4 (1:100, BTIM-4, Millipore Sigma), pp65RelA(S276 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight at 4 C with primary antibody (DM1a, 1:1000, Sigma T6199), washed 3x 5 min in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Mixed glial cultures were treated either with 1 µM (Z)-4-Hydroxytamoxifen (Sigma) dissolved in Methanol (MeOH ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected 4-Hydroxytamoxifen (H6278, Sigma-Aldrich, 1 mg/kg i.p., 3 times) into pups at P7 to P9 ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
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bioRxiv - Biochemistry 2020Quote: ... Extracted membranes were separated from insoluble debris by centrifugation (130,000 × g for 1 h at 4 °C) and incubated for 2h with 4 mL of Ni2+-NTA beads (Novagen) equilibrated in buffer III (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and 0.26g (12.48 x 10−4 mol, 1:4 stoichiometric ratio) cross-linking agent tetraethyl orthosilicate (TEOS – Sigma-Aldrich, 131903) were mixed at 3500rpm for 1 min using a DAC 150FV2-K speedmixer ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin was pelleted by spinning 5 minutes at 10,000 g at 4°C and subsequently incubated for 1 hour at 4°C in HEPES buffer containing 500 units of Benzonase (Millipore) and 2 µg Pol II antibody (ab5095 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Canada) and a DNA damaging growth condition supplemented with a UV mimetic molecule50 4-Nitroquinoline 1-oxide (4-NQO) (Sigma–Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The IMAC pool was diluted 1:4 in 50 mM Tris HCl pH 8 and applied to 4 mL DEAE-Sepharose (Sigma). After washing with 20 mM Tris HCl ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at 4°C with anti-BUB-1 (Desai et al., 2003) and anti-tubulin (1/400, clone DM1α, Sigma Aldrich) in AbDil ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-H3S28phos antibodies from Santa Cruz Biotechnology were used at 1:200 dilution in Corning PBS supplemented with 4 mg ml−1 bovine serum albumin (BSA; Sigma-Aldrich). Cell samples were washed and blocked with Corning PBS containing 10 mg ml−1 BSA and 1.5 mg ml−1 casein (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized cells were clarified for 20 minutes at 21,000 x g at 4°C and incubated for 1 hour with 1 mL bed volume of anti-FLAG M2 affinity gel (Sigma A4596) which had been pre-equilibrated in DBC wash buffer (0.3% DBC ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was done in ATP hydrolysis buffer (1 mM DTT, 4 mM MgCl2, 1 mM PEP, 12 µL LDH/PK enzyme mix (Sigma Aldrich), 50 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... supplemented with 1 mL/L propionic acid and 1 g/L methyl-4-hydroxybenzoate) containing 0.5% erioglaucine dye (Sigma-Aldrich, #861146) and allowed to feed for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Genetics 2024Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (Flag, F7425, Sigma-Aldrich, 1:1000; HDAC2, ab32117 Abcam, 1:1000; acetylated Tubulin, T6793 Sigma, 1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... pH 14), hydrogen chloride solution (c(HCl)=1, pH 1), and buffer solution (pH 4, pH 9) were purchased from Sigma-Aldrich. Methacryloxyethyl thiocarbamoyl rhodamine B (MA-RhB ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt, Germany; SOX9, 1:1000, #AB5535 EMD Millipore, Burlington ...
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bioRxiv - Microbiology 2024Quote: ... is a 1:1 molar mix of FeSO4 and EDTA (ferrous sulfate chelate solution kept at 4°C, Sigma-Aldrich, F0518). However ...
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bioRxiv - Plant Biology 2021Quote: ... 5’ RACE PCR was performed with 2 µl of 1:5 diluted cDNA in a 20 µl reaction with the KAPA2G Robust PCR Kit (KK5501, Sigma-Aldrich, St. Louis, Missouri, USA) buffer B ...
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bioRxiv - Neuroscience 2021Quote: ... Samples for phosphoproteomics (pooled hemispheres of vHC/dHC) were extracted using the same buffer with the addition of 1:100 phosphatase inhibitor cocktail 2 (P5726, Sigma-Aldrich, St. Louis MO, USA), and phosphatase inhibitor cocktail 3 (P0044 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated with the primary antibody for 2 hours at 37°C (α-SMA anti-mouse IgG2a, 1:250, Sigma Aldrich, St-Louis, MO). The fluorescent secondary antibody (Goat anti-mouse IgG ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were seeded at a density of 70–80*103 cells/cm2 on ECM (1%) and fibronectin (2 μg/ml) (Sigma-Aldrich, Saint Louis, MO, USA) -coated plates ...
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bioRxiv - Bioengineering 2020Quote: ... The O/N culture was back-diluted 1:300 in 3 mL fresh Cal18-2 medium85 (Glucidex 12 was exchanged for Maltodextrin DE 13-17 (Sigma-Aldrich, Saint Louis, MO, USA) supplemented with chloramphenicol also in an Axygen 24-deep well plate ...
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bioRxiv - Plant Biology 2020Quote: ... Leaf tissue was ground in extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.2 % Nonidet P-40 [Sigma-Aldrich], 1% plant protease inhibitor cocktail [Sigma-Aldrich], 2 mM 2,2′-dipyridyl disulfide). Cell debris was pelleted at 8,000 x g ...
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bioRxiv - Neuroscience 2020Quote: ... fixed with vacuum grease and kept in physiological Tyrode’s solution for imaging (in mM: 120 NaCl, 2.5 KCl, 30 glucose, 2 CaCl2, 1 MgCl2, and 20 HEPES; Sigma Aldrich; pH = 7.2-7.4; n = 1.33).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 12] Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panels 1 and 2 (EMD Millipore Corporation, Charles, MO), prepared and analyzed according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: Perfluorononanoic acid (PFNA, 97%), 3-Isobutyl-1-methylxanthine (IBMX, ≥ 99%) and 2’,7’-dichlorofluorescin diacetate (DCFH-DA, ≥ 97%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were harvested at 2 dpi and the media was exchanged for 1% IFS in invasion media (DMEM Sigma D2902, 20 mM HEPES, pH 7.4) with auxin or vehicle PBS added ...
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bioRxiv - Plant Biology 2023Quote: ... 150 mg/L cefotaxime] containing 20 mg/L hygromycin B and incubated at 26°oC under conditions of 16-h light (150 μmol photons m-2 s-1) and 8-h dark until roots were established and leaves touched the PhytatrayTM II lid (Sigma-Aldrich, St. Louis, MO, USA). Plantlets were then transferred to soil.
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bioRxiv - Plant Biology 2023Quote: ... We eluted the bound proteins from the affinity beads with 2× SDS-PAGE sample buffer and analyzed the eluates by immunoblotting with anti-GFP (SAB4301138, Sigma-Aldrich, USA, 1:1,000 dilution), anti-FLAG (F1804 ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by a 2-hour incubation in an antibody mixture composed of a 1:1000 dilution of anti-digoxigenin-POD (Roche/Sigma-Aldrich, St. Louis, MO, USA) and a 1:500 dilution of anti-GFP antibody (ab6556 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Ethanol 95% (v/v) and (-)-nicotine hydrogen tartrate salt ((-)-1 -Methyl-2-(3-pyridyl) pyrrolidine (+)-bitartrate salt were purchesed by Sigma Aldrich (St. Louis, MO, USA). The CB1/2 receptor agonist (R)-(+)-[2,3-Dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone mesylate ...