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Citations for Millipore Sigma :
6801 - 6850 of 7005 citations for GATA1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... for 30 min at room temperature and then incubated with rabbit anti-ZLD (1:750) (Harrison et al. 2010), or anti-Tubulin (DM1A, 1:5000) (Sigma, St. Louis, MO), overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... samples were further incubated overnight with secondary antibodies (rabbit or mouse) that were bound to PLA plus or minus complementary probes (Millipore-Sigma, supplementary table S7). The PLA probes were ligated when the proteins were in proximity due to their interaction giving a fluorescent signal as we previously reported (24) ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with secondary antibodies (anti-Rabbit IgG – peroxidase antibody, Sigma Aldrich, A0545; anti-Mouse IgG – peroxidase antibody, Sigma Aldrich, A9044) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2019Quote: New Zealand rabbits were immunized subcutaneously with 0.4 mg human TNFa (Shanghai Primegene Bi-Tech) in complete adjuvant (Sigma Chemical, St. Louis, MO). After the initial immunization ...
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bioRxiv - Molecular Biology 2020Quote: ... Densitometry was performed as previous described.19 Proximity ligations between ephrinb2/EphB4 in flow-aligned cells were conducted by using Duolink® In situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich, MO). Numbers of individual ligations in raw and post-processed images were compared to test the validity of the quantification ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h and incubated with the rabbit monoclonal anti-HA antibody (CST, 3724, 1:500) or mouse monoclonal anti-Flag antibody (Sigma, F3165, 1:100) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
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bioRxiv - Cell Biology 2019Quote: Antibodies used in immunofluorescence and Western blot include: anti-MMP9 rabbit polyclonal antibody (# AB13458) and anti-GAPDH mouse monoclonal antibody (# CB1001) purchased from EMD Millipore (Billerica, MA); anti-Src rabbit antibody (# 2108) ...
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bioRxiv - Neuroscience 2020Quote: Larvae were processed as described (121) using the DuoLink Mouse Rabbit in situ PLA Kit (Sigma-Aldrich, cat. no. DUO92101, St. Louis, MO). Controls were performed with one or the other epitope-tagged transgene absent or with the probes replaced by water during the first PLA step to ensure that signal observed as not background or bleed-through of channels ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rabbit IgG (goat, coupled to Alexa 488, Thermo Fisher, A32731, 1:500 and donkey, coupled to Cy3, Merck Millipore, AP182C, 1:1000), anti-goat IgG (donkey ...
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...
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bioRxiv - Molecular Biology 2020Quote: ... polyclonal rabbit anti-mouse IgG–HRP (A9044, 1:5,000), polyclonal rabbit anti-goat IgG-HRP (A5420, 1:5,000) were from Sigma Aldrich (St. Louis, MO, USA).
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were rabbit anti-tyrosine hydroxylase (Pel-Freez, P40101, 1:1000) and mouse anti-beta III tubulin (Sigma, T8660, 1:2000).
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bioRxiv - Neuroscience 2020Quote: ... The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000, EMD Millipore, #AP132B, RRID:AB_11212148) followed by 1 h with avidin-biotin complex (ABC Vector Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... Western blots were performed according to previously described protocols (Hatzistergos et al., 2019) using antibodies against H3K27me3 (Sigma-Aldrich, rabbit polyclonal, 0.4μg/ml), Isl1 (Rabbit monoclonal ...
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bioRxiv - Neuroscience 2020Quote: ... We incubated free-floating CeA coronal sections (16 µm) for 2 h at 30 °C with rabbit anti-FG antibody (Millipore; AB153I; 1:200) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Neuroscience 2020Quote: ... stained overnight with the primary antibody (Rabbit anti-TH, Sigma Aldrich T8700, 1:500 or Goat anti-ChAT, EMD Millipore AB144P, 1:200), washed ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and then immunostained with a mixture of primary antibodies (Rabbit anti-TH, Pel-freez, p40101-0, 1:500; Chicken anti-GFAP, Millipore, AB5541, 1:500) in a blocking solution at 4°C ...
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bioRxiv - Physiology 2021Quote: ... PLA was performed using the Duolink® In Situ Detection Reagents Red with Duolink® In Situ PLA probe anti-rabbit PLUS and anti-mouse MINUS (Sigma-Aldrich). The nuclei of cells were stained using Duolink® In Situ Mounting Medium with DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Human-αSMA staining was visualised by 1h incubation with a secondary goat anti-rabbit-HRP conjugated antibody followed by a 10 min incubation with 3,3’-diaminobenzidine (DAB Fast Tablet, Sigma-Aldrich, St. Louis, MO). Nuclear counterstaining was performed with hematoxylin after which the sections were dehydrated and mounted with Entellan.
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bioRxiv - Cell Biology 2020Quote: ... incubated in mouse anti-Pax7 antibody (undiluted, Antibody Registry ID: AB_528428) and rabbit anti-laminin (1:250, #L9393,Sigma-Aldrich, St. Louis, MO) overnight at 4°C ...
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bioRxiv - Zoology 2021Quote: ... in PBS and then incubated in the primary antibodies (Rabbit Anti-phospho-histone H3, Sigma-Aldrich H0412 and Mouse Anti-acetylated-α-tubulin, Sigma-Aldrich T6793 ...
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bioRxiv - Neuroscience 2021Quote: ... They were then incubated with secondary antibody (goat anti-mouse Alexa 488 or 555, goat anti-rabbit Alexa 488 or 555, and goat anti-rat Alexa 555, Sigma Aldrich, 1:500) and mounted with Antifade ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... Coronal sections were co-stained with primary antibodies (rabbit anti-Ki67 [LS-C141898, Lifespan Biosciences, Seattle, WA] and guinea pig anti-DCX [AB2253, Millipore, Burlington, MA]), followed by secondary antibodies (Ki67 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked for one hour in 5% normal goat serum in PBST and incubated overnight with rabbit anti-p75NTR (Millipore 07-476, 1:1000) and phalloidin-Alexa Fluor® 488 (Invitrogen A12379 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... 10% normal donkey serum in 1xPBS) and then incubated overnight at 4°C with a rabbit polyclonal antiserum against cFos (1:1000, Millipore and Cell Signaling) followed by a Cy3-conjugated donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 1h in TBST containing 4% dried milk and rabbit anti-MYO10 (1:300, Sigma-Aldrich Ref. HPA024223) as primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# AF-1810), DLL4 (1:25 dilution, Goat polyclonal; Company, Novus, Cat#AF1389) and NG2 (1:200 dilution, Rabbit polyclonal; Sigma-Aldrich, Cat# AB5320). Quantification of radial outgrowth ...
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bioRxiv - Plant Biology 2022Quote: ... a 1:5000 dilution of anti-rabbit IgG secondary antibodies was employed and detected by chemiluminescence with the Luminata pack (Millipore, Billerica MA, USA).
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated overnight at 4 °C with 1:500 rabbit anti-ACP antibody (Gallagher & Prigge, 2010) and 1:500 mouse anti-FLAG antibody (Catalogue no. F3165, Millipore Sigma, MO, USA). The cells were washed with PBS three times and then incubated for 2 h with 1:1000 goat anti-mouse Alexa 488 (Catalogue no ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-MBP (Abcam ab7349, 1:100 for tissue, 1:400 for cultured cells; knockout-validated35, rabbit anti-Olig2 (Sigma AB9610, 1:1000), mouse anti-CC1 (Oncogene OP80 ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Neuroscience 2023Quote: ... eYFP fluorescence was used to confirm TH+ expression in VTA cell bodies using a rabbit anti-TH antibody (1:1000; Millipore Sigma, Burlington, MA) as the primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... thawed at 37 °C and then mixed with defibrinated rabbit blood in a 1:1 proportion and ATP (Sigma-Aldrich, catalog #A2383, USA) in a 3 mM final concentration ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cell bodies were identified by immunolabeling against Glutamine Synthetase using rabbit anti-Glutamine Synthetase antibody (1:500, Sigma-Aldrich catalog# G2781, RRID:AB_259853), and GABA immunoreactivity was detected by immunostaining with guinea pig anti-GABA antibody (1:500 ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were probed with either rabbit anti-RACK1A (1:500) (Chang et al., 2005) or mouse anti-α-Tubulin (1:3000) (Sigma-Aldrich T6074) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... overnight and then with primary antibodies for 48 h at 4°C: rabbit-α-rab3 (1:250; provided by S.J. Sigrist, unpublished) and chicken-α-GFP (1:150, Sigma Aldrich, AB_90890). After a further round of washing ...
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bioRxiv - Pathology 2024Quote: ... The membranes were incubated after washing with an anti-rabbit IgG alkaline phosphatase secondary antibody (1:5000, Sigma– Aldrich, St Louis, MO, USA) for 1 h ...
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bioRxiv - Physiology 2024Quote: ... and the membrane was incubated for 2h at RT with rabbit anti-GAPDH antibody (Sigma Aldrich, G9545, diluted 1:2000 in TBS-T) at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Primaries were prepared in 10% normal goat serum and applied as combinations of either: 1) anti-Aβ1-40 rabbit polyclonal (Millipore Sigma #AB5074P, 1:200) and anti-Aβ1-42 12F4 mouse monoclonal (Biolegend #805502 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were then transferred to a ChAT primary and TrkA primary antibody solution with blocking buffer (1:200 dilution goat anti-ChAT polyclonal AB143, Millipore EMD, Billerica, MA; 1:200 rabbit anti-TrkA polyclonal 06-574, Millipore EMD, Billerica, MA) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections from a subset of animals (n=16) were processed identically as described above with rabbit anti-nNOS (Millipore Cat# AB5380, RRID AB_91824) and the appropriate secondary antibody conjugated to Alexa Fluor 647 ...