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Citations for Millipore Sigma :
6601 - 6650 of 7005 citations for GATA1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500; mouse anti ß-actin, Sigma, 1:7500 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then blocked for at least 1 hour in 5% milk and then incubated overnight rocking with either rabbit polyclonal α-FLAG (1:1000, Sigma) or rabbit polyclonal α-mCherry (1:10,000 ...
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bioRxiv - Biochemistry 2023Quote: Duolink proximity ligation assays were performed using a Duolink™ In Situ PLA Kit Goat/Rabbit (Olink Biosciences) purchased from Sigma. Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... rat monoclonal antibody against the N-terminus of DAT (MAB369) and rabbit polyclonal antibody against tyrosine hydroxlase (TH) (AB152) were from EMD Millipore, Rabbit polyclonal antibodies against vacuolar protein sorting-associated protein 35 (VPS35 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Physiology 2023Quote: ... Collected adrenal sections were probed with antibodies for TLQP-21 (rabbit anti-TLQP-21, 1:100, 20) or Tyrosine Hydroxylase (sheep polyclonal TH, 1:100, Millipore Sigma) overnight at 4°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... blocked in Odyssey Blocking Buffer (Licor #927-50000) at room temperature for 1 hour and incubated with primary antibodies against total Cx43 (1:5000, C6219 rabbit, Sigma Aldrich) and GAPDH (1:5000 ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-rabbit secondary antibody conjugated with horse radish peroxidase enzyme was used in the ratio of 1:10000 (Sigma Aldrich, USA) and blots were visualized using chemiluminescent substrate as described above.
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... in PBS (BSA/PBS-T) for 1 hour prior to washing with PBS and incubation with primary anti-TH rabbit polyclonal antibody (EMD Millipore) at 1:300 BSA/PBS-T overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Processing of brain and brain sections for TH immunohistochemistry was similar to the protocol described above except for primary (rabbit anti-TH antibody 1/200, Millipore ab152) and secondary (Alexa Fluor 555-conjugated donkey anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody was incubated at room temperature for 1 hour (Donkey anti-rabbit IgG antibody: Sigma-Aldrich, SAB3700932; 1:100). Fusion protein ProteinA-Tn5 was prepared in-house (plasmid Addgene #124601 ...
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bioRxiv - Genetics 2023Quote: ... Duolink probes (mouse plus and rabbit minus) were prepared and applied as described by manufacturer’s instructions for the Duolink® PLA kit Cy5 (Millipore-Sigma DUO92013).
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation with an anti-EMILIN-1 rabbit polyclonal antibody (x400, HPA002822; Sigma Aldrich, Saint Louis, MO, USA) in Dako REAL Antibody Diluent at 4°C for 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Physiology 2024Quote: ... Rabbit α-mouse Calmodulin (5μg mL-1, CellSignaling), rabbit α-mouse β-actin (1μg mL-1, CellSignaling) and mouse α-mouse GAPDH (1μg mL-1, Merck/Millipore), goat-α-mouse IRDye 680RD ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed in 0.3% PBST several times and then incubated with AlexaFluor goat anti rabbit 633 (1:250, Sigma Cat# SAB4600141) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002; In-Situ Detection Reagents FarRed, Sigma DUO92013) according to the manufacturer’s in situ fluorescence protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Neuroscience 2024Quote: ... and probed for iFGF12 and iFGF13 expression using validated (Angsutararux et al., 2023; Nerbonne, unpublished) rabbit polyclonal anti-iFGF12 (Rb-α-iFGF12; 1:500; Sigma) and anti-FGF13 (Rb-α-iFGF13 ...
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bioRxiv - Neuroscience 2024Quote: ... all precipitates were solubilized in SDS sample buffer and detected by western blotting with rabbit anti-FLAG (1:1000; F7425; Sigma-Aldrich), rabbit anti-EZH2 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
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bioRxiv - Neuroscience 2024Quote: ... dorsal and ventral transverse slices were incubated with the following primary and secondary antibody combinations using the protocol described: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... For the dual-labeling of Cre and PCP4, guinea pig anti-Cre (1:4000, Zuckerman Institute virus core) and rabbit anti-PCP4 (1:300, #HPA005792, Sigma-Aldrich) were used as the primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with the secondary antibodies Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma-Aldrich, DUO92002-100RXN) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... in Tris-buffered saline with Tween 20 (TBST) and incubated with 1:5000 rabbit anti-FtsA or mouse anti-FLAG (F1804, Sigma-Aldrich) antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti- cytokeratin 8 (TROMA-I; Developmental Studies Hybridoma Bank; 1:500) and rabbit anti- Sox9 (Millipore, Burlington, MA; 1:750). Detection of antibody binding was with the appropriate species-specific Biotin-SP-conjugated goat anti-antibodies (1:400 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibody Donkey anti-mouse 488 (Alexa-fluor A32766, Life Technology), Donkey anti- rabbit 555 (Alexa-fluor A32794, Life Technology) and DAPI (MBD0015, Sigma-Aldrich) in blocking buffer were added to the samples and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... was performed to detect the interactions between ITGβ1 and CD44 using Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The hippocampus was sliced in a transverse orientation and then labeled with PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich) as primary and goat anti-rabbit IgG (Life Technologies) ...
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bioRxiv - Plant Biology 2020Quote: ... two primary antibodies were used: anti-OsCenH3 rabbit antibody and anti-α-tubulin mouse antibody (T6199, Sigma-Aldrich, St. Louis, MO. USA) (Nagaki et al. ...
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bioRxiv - Cell Biology 2020Quote: ... western blot was performed on Sca-1−/low keratinocyte or in keratinocyte cell lysate from back skin of young and old mice for Cdc42 using anti-Cdc42 (Millipore, rabbit polyclonal) antibody and was normalized with the actin blot using the Actin (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were washed 3 times with TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in 1 % BSA solution in TBS-T ...
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bioRxiv - Immunology 2021Quote: ... The membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-PALS-5 diluted 1:1,000 and mouse anti-tubulin (Sigma, catalog number T9026) diluted 1:3000 in blocking buffer) ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381; Mouse anti-β-Tubulin, 1:2000, Sigma T5168) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... for 1h at room temperature before incubation in primary antibodies (rabbit anti-Synapsin I, ab64581, Abcam; guinea pig anti-GLT-1, ab1783, EMD Millipore; mouse anti-FLAG, F1804, Sigma-Aldrich) at 1:1000 in block for 48h ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were incubated in blocking buffer with primary antibody (Rabbit α-β-III Tubulin, Millipore Sigma T2200-200UL, 1:1000, RRID: AB_262133) overnight at 4°C without agitation ...
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bioRxiv - Biophysics 2022Quote: Native thin filaments were purified from rabbit right and left ventricular cardiac muscle that had been glycerinated and actin was purified from acetone powder of rabbit skeletal muscle (Sigma-Aldrich, USA), following a protocol previously used in our laboratory (Matusovsky et al. ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal, Sigma-Aldrich), anti-C1qbp (PAA651Mu01/Hu01, Cloud-Clone Corp., TX, USA) anti-β-actin (A3854, rabbit polyclonal, HRP-conjugate, Sigma-Aldrich), anti-V5 agarose affinity gel (A7345 ...
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bioRxiv - Developmental Biology 2019Quote: ... The blot was cut in half just above the 41K marker and the top half of the membrane was probed with rabbit anti-tubulin (Sigma, Lot#50K4813) followed by goat anti-rabbit IgG (HRP ...
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bioRxiv - Biochemistry 2019Quote: ... Blots were probed for 1 hour at room temperature with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Sigma-Aldrich; A0545) diluted 1:10000 in the same buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal antibody against EphB1 sequence (AA 528-541, DDDYKSELREQLPL) (anti-EphB1 pAb) was custom produced by Sigma-Aldrich (St. Louis, MO). Anti-EphB1 mouse monoclonal against EphB1 sequence (AA 528-541 ...
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bioRxiv - Biochemistry 2019Quote: ... Blots were probed for 1 hour at room temperature with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Sigma-Aldrich; A0545) treated with enhanced chemiluminescence reagent (Thermo Fisher ...