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Citations for Millipore Sigma :
6551 - 6600 of 7005 citations for GATA1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were stained for c-Fos using rabbit anti-c-Fos antiserum (ABE457; Millipore, 1:1000; 1% NDS; 0.3% TxTBS) with Alexa Fluor 488 conjugated donkey anti-rabbit antiserum (AF488 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS and incubated with secondary antibodies at 1:500 dilution (goat anti-rabbit Alexa 594, Sigma Aldrich,) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... or LY (1:500, #A5750, made in rabbit)-- and anti-sera to TH (1:10,000, MAB318, EMD Millipore Corp, Temecula, CA) or GAD1 (1:10,000 ...
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bioRxiv - Plant Biology 2024Quote: ... 2003) and a goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (1:4000, Chemicon®, Sigma-Aldrich, www.sigmaaldrich.com, #AP307P). Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently these cysts were washed three times with PBS and incubated with secondary anti-rabbit IgG conjugated with Alexa flour 488 (1:300) and Calcofluor White 1:20 (CFW, 1 mg/ml, Sigma Aldrich) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunostained with rabbit anti-SON antibody (1:200 dilution, Novus) or mouse anti-SRRM2 antibody (1:200 dilution, Sigma Aldrich) for 1h at room temperature followed by 3 times wash with DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were incubated in mouse anti-FasII (1:200, Developmental Studies Hybridoma Bank) and rabbit anti-myc (1:1000, Sigma-Aldrich) diluted in blocking buffer for 16 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for immunoblotting in this study include rabbit polyclonal anti-GFP (ab290, Abcam, 1:2,500 or Sigma, G1544, 1:1000), mouse monoclonal anti-GFP (JL8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse monoclonal anti-Flag (M2), mouse monoclonal anti-Actin (MAB1501, clone C4) and rabbit polyclonal anti-GST (G7781) were from Sigma-Aldrich. Rabbit polyclonal anti-FOXK1 (MNF ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in blocking solution (5% normal donkey serum and 0.3% Triton X-100 in PBS) for 30 minutes and then incubated in rabbit anti-TH polyclonal antibody (Millipore, Cat: AB152) diluted 1:1000 in 0.3% Triton X-100 in PBS ...
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bioRxiv - Microbiology 2024Quote: ... HRP-conjugated anti-mouse secondary antibody (57404) was purchased from SRL and anti-rabbit secondary antibodies were purchased from Sigma (A8275). Alexa Fluor 488 goat anti-mouse (A11001) ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA Probe anti-Mouse PLUS and anti-Rabbit Minus (Sigma-Aldrich). The incubation with all antibodies was carried out in a humidified chamber for 1 h at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP ...
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bioRxiv - Microbiology 2024Quote: ... α-PilT (66) (dilution 1:2000) and α-PilB (66) were used together with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Sigma) as a secondary antibody (dilution 1:10000) ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with horse-radish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit-HRP and goat anti-mouse-HRP, Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated for 1 hour using the following antibodies: 1:500 rabbit anti-H3S10ph (Sigma-Aldrich, Merck, 06-570), 1:500 mouse anticyclin B1 (BD Pharmingen ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with PBS 1x-1% non-fat milk overnight at 4°C and incubated in primary antibody anti-Orco (1:500) and the secondary antibody Goat anti-rabbit IgG HRP conjugate (1:5000 Sigma-Aldrich) for 1 hour each ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit IgG and visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Burlington, MA) and an ImageQuant LAS4000 digital imaging system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then blocked for at least 1 hour in 5% milk and then incubated overnight rocking with either rabbit polyclonal α-FLAG (1:1000, Sigma) or mouse monoclonal α-RpoA (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA (rabbit monoclonal from Cell Signaling Technology Inc., cat. no. 3724S; and mouse monoclonal from Sigma-Aldrich, cat. no. H3663), anti-myc (mouse monoclonal clone 9E 10 from the University of Iowa Hybridoma Bank) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... Revelation of the primary antibodies was performed by incubation with goat anti-rabbit Horseradish peroxidase-conjugated secondary antibody (Ref#A0545, Sigma-Aldrick) at 1/10000 ratio in blocking solution for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Cytoplasmic and nuclear fractions were then subjected to western blotting and labelled with rabbit monoclonal anti-GAPDH (Sigma, G9545, 1:5,000) antibody and rabbit polyclonal anti-LSD1 antibody (Abcam ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... pTyr (clone 4G10, 05-321), and HA (clone HA-7, H3663) and rabbit polyclonal anti-SEPTIN12 (HPA041128) were from Sigma-Aldrich. Rabbit and mouse monoclonal anti-DYKDDDDK (clone D6W5B ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBS again and incubated for 48h with a rabbit anti-TH antibody (1:1000, Millipore Cat# AB152, RRID:AB_390204) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated in primary antibody solution containing Anti-Neurofilament 200 primary antibody produced in rabbit IgG (N4142, Sigma Aldrich) and biotin-conjugated Anti-NeuN primary antibody produced in mouse IgG (MAB377B ...
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bioRxiv - Genetics 2023Quote: Liver protein isolation and western blots on liver extracts and plasma samples were performed as previously described.17 Primary antibodies to the 2A peptide (1:5000, rabbit, ABS31, Sigma-Aldrich), GFP (1:3000 ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Microbiology 2023Quote: PLA: PLA was carried out using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, Cat #DUO92101-1KT). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... For secondary antibody incubation we used a goat-anti-guinea pig Alexa 488 (1:1000) and a goat-anti-rabbit Atto 647N (1:1000, #40839, Sigma Aldrich). After antibody incubation ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated in primary antibody solution (1:1000 rabbit anti-GFP or 1:1000 rabbit anti-RNA Pol II CTD repeat YSPTSPS (phospho S5) in blocking buffer (1:10 Western Blocking Reagent (Sigma 11921673001) in PTx) ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568/AttoN647-conjugated goat anti-mouse or anti-rabbit (1:100–1:400, Thermo Fischer Scientific) and Alexa 488/568-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...