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Citations for Millipore Sigma :
6701 - 6750 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was quantified by Bradford method and immunoblot analysis was performed using anti-HA antibody (1:1000 dilution, Sigma). Immunoblot using anti-αTubulin antibody (1:1000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Neuroscience 2021Quote: ... ConA-bead bound cells were incubated overnight in 50μL Antibody Buffer (Wash Buffer, 0.01% digitonin, 2mM EDTA) with 1:100 rabbit anti-FEV (Sigma-Aldrich #HPA067679), guinea pig anti-Lmx1b (Carmen Birchmeier ...
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bioRxiv - Neuroscience 2021Quote: ... One set of slices were stained with primary antibodies for mouse monoclonal anti-GFAP (G3893; Sigma-Aldrich; 1:1,000 dilution) and rabbit anti-Iba1 (019-19741 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-BK 1:1,000 (Antibodies Inc AB_224953) and after striping a mix of anti-γ-tubulin 1:10,000 (Sigma-Aldrich,T5326,) and anti-PSD95 1:5,000 (Antibodies Inc AB_224953) ...
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bioRxiv - Neuroscience 2021Quote: The primary polyclonal antibodies applied overnight at 4 °C were as follows: mouse anti-UBE3A 1: 600 (SAB1404508, Sigma, USA); rabbit anti-ELAVL4/HudD 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibody used in this study was rabbit anti-PH3 (1:000, EMD Millipore, Burlington, MA, cat. 06-570). The secondary antibody used in this study was donkey anti-rabbit-555 (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... separated on 10% SDS PAGE for analysis and detected by western blot using monoclonal anti-polyHis antibody (Sigma Aldrich, Israel).
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bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... were fractionated by SDS-Page 10% and transferred to nitrocellulose membrane for immunoblot analysis using rabbit anti-GRP78 antibodies (GL-19, 1:5000 dilution from Sigma), anti-PERK antibodies (CS56833 ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were transferred to a PVDF membrane followed by incubation with Anti-Cas9 antibody conjugated to FITC (Sigma Aldrich). Anti-mouse secondary antibody conjugated to Alkaline phosphatase was used to develop the blot with NBT-BCIP (nitro-blue tetrazolium and 5-bromo-4-chloro-3’-indolyphosphate).
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bioRxiv - Immunology 2019Quote: ... Samples were then blocked in PBS with 0.5% Triton X-100 solution supplemented with 2% goat serum for 1 h at room temperature and incubated overnight at 4 °C with mixtures of the following primary antibodies: anti-centrin (clone 20H5; Millipore), anti-pericentrin (ab4448 ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibody and chemical staining were performed in blocking solution at the following dilutions: anti-β-catenin 1:500 (Sigma C2206), anti-laminin 1:500 (Sigma L9393) ...
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bioRxiv - Neuroscience 2019Quote: ... and corresponding PVDF membranes were incubated overnight at 4°C with primary antibodies: mouse anti-ATXN3 (1H9) (1:2000, MAB5360, Millipore), rabbit anti-MJD [22] (1:20000) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used include anti-α and β-Synuclein (EP1646Y, N-terminal rabbit monoclonal, 1:100 dilution; Millipore, Billerica, MA), anti-pS129 αSyn (81A ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594-conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were blocked with 5% (w/v) bovine serum albumin in PBS and then incubated with 1:50 anti-HA mouse monoclonal antibody (Sigma) in PBS at room temperature for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Attachment of actin filaments stabilised by biotin-phalloidin was performed in channels that were incubated with an anti-biotin antibody solution (1 mg/ml in PBS, B3640, Sigma) prior to passivation ...
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bioRxiv - Cancer Biology 2019Quote: ... hybridization buffer contained 1:100 dilution of TERT exon and intron probes and 1:800 dilution of primary antibody (mouse M2 monoclonal anti-FLAG, F1804, Sigma), and (ii ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Biophysics 2019Quote: ... incubated again for 1 hour at 37°C with 1:400 mouse anti-heavy chain clathrin antibody (Sigma Aldrich, C1860) in PBS + 1% BSA and washed three times with PBS + 1% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with 0.5% Triton and blocked with 0.5% Triton and goat serum blocking solution before being incubated in mouse anti-PV monoclonal antibody (1:2000, Sigma-Aldrich) and mouse anti-NeuN polyclonal antibody (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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bioRxiv - Molecular Biology 2019Quote: ... The activated beads were resuspended in 100 μL 50 mM MES (pH 5.0) and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804 ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Neuroscience 2019Quote: ... Signals were obtained by binding of a secondary anti-rabbit horse radish peroxidase (HRP) linked antibody (1:15000, Sigma-Aldrich) and visualized by exposing the membrane to a charge-coupled device camera (LAS1000 ...
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bioRxiv - Neuroscience 2019Quote: Membranes were blotted with primary antibodies including rabbit polyclonal anti-GluN1 C- terminus (1:2000, Sigma-Aldrich Cat# G8913, RRID:AB_259978), rabbit polyclonal anti-GluN2A N-terminus (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... Western blots were performed using standard methods and the following antibodies: Mouse anti-FUS (AMAB90549 Sigma Aldrich, 1:500 dilution), rabbit anti-GFP (sc-8334 Santa Cruz ...
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bioRxiv - Molecular Biology 2019Quote: ... blots were subsequently incubated for 1h at room temperature with horseradish peroxidase-conjugated secondary anti-rabbit/mouse antibodies (1:10000; Sigma). Immunoreactive bands were visualized by chemiluminescence detection of peroxidase activity (SuperSignal West Dura ...
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bioRxiv - Neuroscience 2020Quote: ... The phenotype of IdU-IR cells and CldU-IR cells was determined using rabbit anti-calbindin antibodies (1/200, Millipore) that were revealed with Alexa 488-goat anti-rabbit IgG antibodies (1/500 ...
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bioRxiv - Plant Biology 2020Quote: ... Four washes of 15 min each in TBST at room temperature and then it was incubated two hours with a secondary antibody anti-rabbit (goat) conjugated with alkaline phosphatase (A3687, Sigma), in a 1:2,500 dilution with TBST ...
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bioRxiv - Neuroscience 2021Quote: ... Some sections were also incubated with a combination of a mouse monoclonal anti-GFAP antibody (1:1,000; G3893, Sigma-Aldrich) and the above-described polyclonal anti-GFAP antibody to verify whether both antibodies provided similar detections of GFAP protein ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then incubated overnight at 4°C with a goat primary antibody anti-ChAT (1:500, Catalog #AB144, Millipore) and 2.5% donkey serum in PBS-T with gentle rocking ...
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bioRxiv - Cancer Biology 2020Quote: ... Total protein lysates obtained from transfected cells were used for Western Blot analysis using the following antibodies: anti-MYB mouse monoclonal (1:1000, Upstate/Millipore) and anti-GAPDH mouse monoclonal (1:10000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... and goat serum (15 min, Maixin, Fuzhou, China) and then incubated with rabbit anti-TH antibody (1: 1,000, AB152, Millipore, USA) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: Frozen skin tissues from a normal individual and the patients were sectioned at 6 μm and stained with mouse monoclonal anti-NC1 C7 antibody (clone LH 7.2; Sigma-Aldrich) at a 1:1000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by two 10 min washes and a 1 h incubation in peroxidase-labelled anti-mouse antibody (1:2000, Sigma). Membranes were developed by homemade enhanced chemiluminescence and scanned using FluorChem M hardware (Proteinsimple).
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bioRxiv - Bioengineering 2020Quote: ... The membrane was blocked by 5% fat-free milk dissolved in TBS/0.05% Tween-20 for 1 hr then cut off according to the marker and incubated with anti-flag monoclonal antibody (Sigma) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: Immunofluorescence was done as previously described (Yellajoshyula et al., 2017) for the following antibodies: Rat anti-MBP (Millipore, Cat# MAB386), Mouse anti-O4 (kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min prior to incubation for 1 h in primary antibodies diluted in blocking buffer: Goat anti-mouse GAPDH (RRID: AB_1078991, Sigma-Aldrich) 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... In three rats brain stem sections were first immunoreacted with a goat anti-choline acetyltransferase antibody (1:1000; Millipore Sigma) and a rabbit anti-goat IgG conjugated to horseradish peroxidase (Vector Labs) ...
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bioRxiv - Developmental Biology 2020Quote: ... cryosections were incubated overnight at 4°C with the following primary antibodies: rat monoclonal anti-Neural Cell Adhesion Molecule L1 (1:500 Millipore, Cat # MAB5272 ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies or lectin were used: an anti-aggrecan rabbit antibody (1:4500 for DAB staining; 1:500 for fluorescence staining; AB1031, Merck Millipore), an anti-GABA chicken antibody (1:200 ...