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Citations for Millipore Sigma :
6501 - 6550 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Immunology 2021Quote: ... purified resting B cells were cultured at a density of 1.5-2× 106/ml in B cell culture medium supplemented with 25mg anti-IgM antibody (Millipore) and 2ng/ml of IL-4 (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... A 5 μm section was deparaffinized and stained with anti-MAP2 antibody (Sigma-Aldrich, 1:1000 dilution, Cat# M4403) (the details of this procedure are described elsewhere (Shichita et al ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Cell Biology 2021Quote: ... the following primary antibodies were used at the indicated dilutions: mouse anti-γ-tubulin 1:500 (T5326; Sigma Aldrich), rabbit anti-mCherry 1:500 (ab167453 ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Biochemistry 2020Quote: All antibodies were from commercial sources: anti-FLAG M2 peroxidase was obtained from Sigma Aldrich (F4042, 1:1,000 dilution), anti-HIF1α was from BD Biosciences (610958 1:1,000 dilution) ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000 ...
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bioRxiv - Genetics 2020Quote: ... blots were incubated with the following antibodies: monoclonal mouse anti-GFP (Sigma, MO; clone 7.1 and 13.1, 1:1000), mouse anti-Tubulin (DHSB ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated at 4 °C with gentle rotation for 30min and centrifuged at 13,000 RPM for 10 min to collect the supernatant for WB using Anti-Flag antibody (F1804, Sigma). For the WB using chromatin after the biotinylation reaction ...
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bioRxiv - Microbiology 2022Quote: ... followed by 4x 8 min in PBS and then incubated in PBS-5% dried semi-skimmed milk with 1:10,000 anti-mouse HRP-linked secondary antibody (A9044, Sigma) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and then for 1 h at room temperature with a mix of the two primary antibodies directed against the target proteins (anti-biotin to detect new H3.3-SNAP-biotin and anti-gH2A.X to detect sites of DNA damage) diluted in antibody dilution reagent (Sigma-Aldrich). Coverslips were then incubated for 1 h at 37 °C with secondary antibodies each harboring a PLA probe (Duolink In Situ PLA MINUS/PLUS probes ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6 ...
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bioRxiv - Cell Biology 2022Quote: ... Postnuclear supernatants were incubated for 2 hours with pre-equilibrated 20ul anti-HA antibody magnetic beads slurry (Sigma-Aldrich). After three 10min-washes in 0.2% DMNG washing buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Postnuclear supernatants were incubated for 2 hours with pre-equilibrated 30ul anti-HA antibody magnetic beads slurry (Sigma-Aldrich). After three 15min-washes in RIPA buffer on a rotating wheel at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Secondary incubation was performed with 1:20 000 dilution of HRP-conjugated anti-human IgG antibody (Sigma Aldrich SAB701283) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then washed with TBST three times for 10min before incubating with a second antibody (anti-rabbit (Sigma), 1:2000 dilution ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 3-5 times with 1x TBST for a total of 20 min and then incubated for an hour with 1:3,000 dilution of secondary antibody (goat anti-mouse alkaline phosphatase conjugate, Sigma). The membrane was washed again with 1x TBST 3-5 times for a total of 20 min and developed using 5-bromo-4-chloro-3-indolylphosphate (BCIP)/ nitro-blue tetrazolium (NBT ...
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bioRxiv - Pathology 2022Quote: ... and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94,Sigma-Aldrich, St. Louis, MO) or 1:1000 anti- CD163 monoclonal antibody (Novus Biologicals ...
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Local induction of IAA5 and IAA29 promotes DNA damage-triggered stem cell death in Arabidopsis rootsbioRxiv - Plant Biology 2022Quote: ... Chromatin bound to SOG1-Myc proteins extracted from whole seedlings was precipitated with anti-Myc antibody (clone 4A6, Millipore). To quantify the precipitated chromatin ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were washed and then treated with a 1:1000 dilution of mouse anti-FLAG antibody (clone M2, Sigma) for 60 minutes then washed 3x in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... after RNAscope detection of C3 some slides were co-labelled with an anti-GFAP antibody (1:400, mab360, Millipore) according to the immunohistochemistry methods.
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bioRxiv - Developmental Biology 2022Quote: ... Every 10 μg of chromatin was incubated with 2 µg of mouse M2 anti-FLAG monoclonal antibodies (Sigma-Aldrich) overnight at 4° C on a nutator ...
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bioRxiv - Physiology 2023Quote: ... and subsequently used for IP with 48 μg of an anti-NaVPAN mouse monoclonal antibody (mαNaVPAN, Sigma-Aldrich, #S8809), raised against the SP19 epitope (Vassilev et al. ...
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bioRxiv - Neuroscience 2023Quote: ... CN neurons were labeled with guinea pig anti-NeuN primary antibody (1:500, abN90, Millipore, Burlington, MA, United States) and rabbit anti-GFP primary antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The brain slices containing HVC were collected and incubated overnight with a rabbit anti-GFP antibody (EMD Millipore, AB3080P) in 1xPBS containing 10% donkey serum and 0.2% Triton at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Pellets were resuspended in 500μl DPBS and incubated in BSA (final concentration 0.1%) and anti-NeuN antibody (1:1000, Alexa488 conjugated, Millipore, MAB377X) under rotation for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were incubated with 15 μg of mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich, F1804, RRID: AB_262044) in 1 mL of PBS containing 0.5% BSA and 0.1% Triton X-100 at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were blocked for 4 hours in 5 % donkey serum in PBST (0.3 %) before incubation in the primary antibody diluted in blocking solution overnight (goat anti-VAChT, Millipore 1:2000 LOT ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the brain slices were washed 3 times using 1 x PBS in a shaker and incubated in blocking solution (10% normal goat serum and 0.2% Triton X-100 in PBS) for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a ...
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bioRxiv - Physiology 2022Quote: ... followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose, liver: 1:500; pancreas: 1:800; Sigma, cat #HPA002118) in antibody diluent (Agilent ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Plant Biology 2024Quote: ... followed by overnight incubation at 4°C with a 1:5000 dilution of anti-FLAG antibody (Sigma, Cat# F1804) in the blocking solution ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with goat anti-rabbit AP-conjugated antibodies (1:5000 in PBS, Sigma-Aldrich, Missouri, USA), followed by four washes with PBS-T ...
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bioRxiv - Pathology 2024Quote: ... Membranes were incubated in 5% non-fat dry milk for 1 h and then overnight with primary antibody anti-FGF21 (1:1,000, rabbit, SAB2108136, Sigma), anti-FXR (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... as the primary antibodies and Alexa Fluor 488 goat anti-rabbit IgG (Sigma-Aldrich, 11034, 1:1000 for IF), Alexa Fluor 488 goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed with anti-FLAG polyclonal primary antibody produced in rabbit (Sigma-Aldrich Cat# F7425, RRID: AB_439687) overnight at 4° C ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with anti-FLAG polyclonal primary antibody produced in rabbit (Sigma-Aldrich Cat# F7425, RRID: AB_439687) at 4° C overnight Then the membrane was washed 3 times in PBS-T (0.5% tween 20 in 1 x PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... The blots were re-probed with monoclonal anti β-actin antibody (1:50000, Sigma-Aldrich, Cat # A5441, lot. 116M4801V). Statements of validation and references are on the manufacturers’ websites ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...