Labshake search
Citations for Millipore Sigma :
6451 - 6500 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... The mouse brain endothelial cell line bEnd.3 was cultured in DMEM medium (Sigma-Aldrich) supplemented with 10% FBS ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 mM phosphoenolpyruvate (PEP) (Molecula) and 16 U/ml lactic dehydrogenase/pyruvate kinase enzymes (Sigma). 24 µl reactions were prepared in 384 well plates (Greiner ...
-
bioRxiv - Neuroscience 2024Quote: ... slices were incubated for 3 min with Hoechst 33342 dye (Merck Millipore, Billerica, MA, USA) (2µg/ml ...
-
bioRxiv - Bioengineering 2024Quote: ... followed by 10 min quenching of endogenous peroxidase activity with 3% hydrogen peroxide (Sigma-Aldrich), and a final blocking was performed with Background Sniper (Biocare Medical ...
-
bioRxiv - Cell Biology 2024Quote: ... Gel fragments were incubated 3 times with 100 mM ammonium bicarbonate (Sigma-Aldrich; cat. #09830) to wash away excess SDS and change the buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated with 25% polyethylene glycol 8000 (PEG-8000, 25322-68-3, Sigma Aldrich, MO) at 4°C overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... 6 or 8 h with 500 µM Indole-3-Acetic Acid (IAA, Sigma-Aldrich #I5148). For induction of Oct4 knockdown in ZHBTc4 cells ...
-
bioRxiv - Neuroscience 2024Quote: ... 40 μm brain sections were washed 3 times with 0.3% Triton X-100 (Sigma-Aldrich) in 1X PBS (PBST ...
-
bioRxiv - Biophysics 2024Quote: ... were activated for 5 min with a solution of 3-(Trimethoxysilyl)propyl methacrylate (Sigma-Aldrich, 440159 ...
-
bioRxiv - Neuroscience 2024Quote: ... 0,2% sodium taurodeoxycholate hydrate and 3 mM 4-methylumbelliferyl β-D-glucopyranosidase (Sigma-Aldrich, M3633) and incubated at 37°C for 90 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... the 96-well plate was immersed in 2% (v/v) 3-mercaptopropyl trimethoxysilane (Sigma-Aldrich) in methanol for 2 minutes to functionalize the polystyrene with silane functional groups ...
-
bioRxiv - Biochemistry 2024Quote: ... N-(3-oxododecanoyl)-L-homoserine lactone (AHL-C12) and 6-methoxyflavanone were purchased from Sigma. Stock solutions of these compounds (DPH ...
-
bioRxiv - Biochemistry 2024Quote: ... suberic acid bis(3-sulfo-N-hydroxysuccinimide ester) sodium salt (BS3) was from Sigma (#S5799), 4,4’-bis(phenylamino)-[1,1’-binaphthalene]-5,5’-disulfonic acid (bis-ANS ...
-
bioRxiv - Molecular Biology 2024Quote: ... in a ratio of 1:3 (μg DNA/μg PEI) in DMEM (Sigma-Aldrich, D6429) without supplements ...
-
bioRxiv - Biophysics 2024Quote: Thoraces from 2–3-day-old adult Drosophila were dissected in 4% paraformaldehyde (PF, Sigma), and indirect flight muscles were isolated as described previously (Katti et al ...
-
bioRxiv - Zoology 2024Quote: ... Worms were anesthetized in tricaine (15% ethyl 3-aminobenzoate, methanesulfonic acid salt; Sigma-Aldrich #E10521), and mounted on microscope slides with a 20×20 mm sticker grid with 1mm resolution (ThomasScientific ...
-
bioRxiv - Plant Biology 2019Quote: ... Cells were transfected with pcDNA3.1 and pEFl vectors using GeneJuice transfection regent (Merck Millipore, #70967-3) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo, Millipore, #WBLUR0100) for chemiluminescence detection using ChemiDoc™ MP Imaging System (Bio-Rad).
-
TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... and the Arp2/3 inhibitors CK-548 and CK-636 were from Sigma (St. Louis, MO). IL-4 was from Genscript (Piscataway ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to stagetips containing 3×14G plugs of 3MTMEmporeTM SDB-RPS substrate (Sigma) as described previously (Kulak et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... washed 3 × 5 mins in phosphate-buffered saline (PBS) with 0.1% Tween-20 (both Sigma-Aldrich) and probed for 1 hour at room temperature in the dark with IRDye® 800CW conjugated goat anti-mouse secondary antibodies (LiCOR Biosciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were washed in PBS and incubated in 3% bovine serum albumin (BSA) (Sigma-Aldrich) diluted in PBS for 1 hour ...
-
bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... shLmnA (produced in our lab), shLMNC (produced in our lab, 5′-TCTCCCACCTCCATGCCAAAG-3’) or shLMNB1 (Sigma clone NM_010721.1-956s1c1 ...
-
bioRxiv - Cell Biology 2020Quote: ... washed and developed with chromogenic 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, Sigma-Aldrich, B6149).
-
bioRxiv - Cell Biology 2020Quote: ... organoids were rinsed twice with warm PBS and incubated with warm PBS containing 3% formaldehyde (Sigma) for 20 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... Bead solution on the column was incubated with 3 µL 50U Thrombin (EMD Millipore, Novagen®) in 250 µL wash buffer overnight at 4° C on a rotator ...
-
bioRxiv - Developmental Biology 2021Quote: ... After 3 washes for 5min each with 1ml DPBS−/− (DPBS without Mg and CaCl2, Sigma, D8537), 1ml 4% paraformaldehyde (PFA ...
-
bioRxiv - Microbiology 2021Quote: ... Freshly purified NyxB was concentrated to 21 mg/ml on 3 KDa Amicon Ultra concentrators (Millipore). SeMet-NyxB was produced in M9 minimum medium and purified as above ...
-
bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2021Quote: ... for phosphorylated histone 3 antibody staining (Chen et al., 2020) (pH3, Sigma # 05-806, 1:100), or with Lavdovsky’s fixative (3.7% formaldehyde ...
-
bioRxiv - Neuroscience 2020Quote: ... then permeabilized for 30 min in PBS containing 1.5% TritonX100 and 3% hydrogen peroxide (Sigma; #31642). Then ...
-
bioRxiv - Developmental Biology 2020Quote: ... After pre-incubation with 3% skim milk in PBS-T (PBS with 0.1% Tween20 (Sigma-Aldrich)) at RT for 30 min ...
-
bioRxiv - Cell Biology 2022Quote: ... the supernatant was added to 3 ml anti-Flag (M2)-agarose (Sigma, St. Louis, MO, USA), and incubated at 4°C overnight ...
-
bioRxiv - Physiology 2020Quote: ... and T3 (3,3’,5-triiodo-L-thyronine, > 95% HPCL, CAS number 6893-02-3, Sigma-Aldrich) were first dissolved in 0.1M NaOH and then diluted in 0.9% NaCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 3 μl were spotted onto plates with or without 100 μg/ml terbinafine (Sigma-Aldrich). Plates were imaged after 3–7 days of incubation at 30°C.
-
bioRxiv - Molecular Biology 2020Quote: ... Following 3 x TBS-T washes the membranes were developed with Immunoblot Forte Western HRP (Millipore), according to the manufacturer’s instructions and imaged using an Amersham™ Imager 600 (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168] ...
-
bioRxiv - Cell Biology 2022Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used for cell viability (Sigma, Israel).
-
bioRxiv - Developmental Biology 2022Quote: ... Slices were washed in PBS for 3 times and incubated in 0.3% TritonX-100 (Sigma-Aldrich,) at room temperature (RT ...
-
bioRxiv - Immunology 2021Quote: ... 3 μg pRSV-Rev and 6 μg of pRRL lentiCRISPR vectors using Poly(ethyleneimine) (PEI; Sigma). Media was replaced 24 hours post-transfection and harvested 24 hours later for filtration with a 0.45 μm filter (SteriFlip ...
-
bioRxiv - Biochemistry 2019Quote: ... Fractions containing α -syn were pooled and concentrated using a 3-kDa Amicon Ultra column (Millipore). Concentrated α -syn was loaded onto a gel filtration column (Superdex 200 ...
-
bioRxiv - Immunology 2019Quote: ... cells were lysed in phosphoprotein lysis buffer containing 0.25% CHAPS with phosphatase inhibitor cocktail 3 (Sigma), protease inhibitor tablet (Qiagen ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were washed 3 times and incubated for 30 minutes with streptavidin Cy3 (Sigma, 1:500). All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako ...
-
bioRxiv - Developmental Biology 2020Quote: ... HDLECs were incubated in a 15-mL conical tube in presence of cytodex 3 beads (Sigma) at a ratio of 400 cells per bead in EGM-2MV medium (Lonza ...
-
bioRxiv - Cell Biology 2019Quote: ... All cultures were tested every 3 months for mycoplasma contamination using the LookOut kit (Sigma, MP0035).
-
bioRxiv - Cell Biology 2019Quote: ... Cultures were then treated with 50 ng/mL LMB dissolved in 7:3 MeOH:H2O solution (Sigma) for 45 minutes alongside a control of the equivalent volume of MeOH before imaging.
-
bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in permeabilization buffer (3% (w/v) bovine serum albumin (BSA, Sigma-Aldrich, Germany) with 0.5% (v/v ...
-
bioRxiv - Immunology 2019Quote: ... the plates were incubated with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (A3219; Sigma-Aldrich) at room temperature for 30 min ...
-
bioRxiv - Neuroscience 2019Quote: ... Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP) (C2920) and 3-NP (N5636) were purchased from Sigma-Aldrich. CHX was dissolved in 95% ethanol and used at 100 µM for indicated time point ...