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Citations for Millipore Sigma :
601 - 650 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and 1:200 Protease Inhibitor Cocktail Set III (all chemicals purchased from Sigma Aldrich)) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2022Quote: ... Another set of clean coverslips was treated with poly-L-lysine solution (Sigma-Aldrich) for 45 min at 37ºC in a humid chamber to activate the surface ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET) to remove DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10 μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125 μl dNTP mix (concentration of each nucleotide was 10 mM ...
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bioRxiv - Cell Biology 2020Quote: ... All primers were synthesized by Sigma-Aldrich (St. Louis, USA) and purified by HPLC ...
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bioRxiv - Molecular Biology 2021Quote: ... Predesigned KiCqStart SYBR Green optimized primers from Sigma-Aldrich (KSPQ12012) were used for RT-qPCR.
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bioRxiv - Developmental Biology 2020Quote: ... The primers were ordered from Sigma (0.05 μmoles and desalted) with a length varying between 90-150bps (the sequence is given in supplemental table 4) ...
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bioRxiv - Molecular Biology 2019Quote: ... Long homology arm introducing primers were ordered from Millipore Sigma, PAAG.
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bioRxiv - Neuroscience 2020Quote: ... primer pairs were obtained from a commercial vendor (Sigma-Aldrich) and primer specificity was tested with adult rat whole-brain cDNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primers used in this work were provided by Sigma-Aldrich Co (St ...
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bioRxiv - Microbiology 2020Quote: ... Reverse Primer 5’-TGGTTGAGCACAGGGTACTT-3’] were synthesized by Sigma-Aldrich, USA ...
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bioRxiv - Cancer Biology 2020Quote: ... and non-universal primers (Thermofisher Scientific, Sigma-Aldrich or Eurofins) designed using Primer3web online tool ...
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bioRxiv - Microbiology 2022Quote: ... Custom primers in this study were made by Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: Mastermix was prepared using forward and reverse primers (Sigma, UK) and where possible a labelled probe for each gene (Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... 0.25 μM each of RamF6 and RamR6 primers (Sigma, Australia), 0.15 μM of molecular beacon Ram6 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... All primers in this study were obtained from Sigma-Aldrich, and the restriction sites are underlined ...
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bioRxiv - Developmental Biology 2019Quote: ... All primers were synthesized by Sigma-Aldrich (St Louis, MO), and gene expression was normalized to 18s rRNA by the ΔΔCT method.
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bioRxiv - Physiology 2021Quote: ... Telomeric forward and reverse primer sequences (Sigma, The Woodlands, TX) were (5⍰→3⍰ ...
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bioRxiv - Microbiology 2021Quote: ... in a thermocycler with desalted oligonucleotide primers (Sigma-Aldrich, UK) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Single gRNA oligos and primers were purchased from Sigma Aldrich, and cloned into the NotI site of plasmids ...
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bioRxiv - Genomics 2021Quote: ... Primers were synthesized by Integrated DNA Technologies (IDT; Sigma-Aldrich). Sequences of designed primers were as below:
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bioRxiv - Cell Biology 2020Quote: ... All primer sequences used were purchased predesigned from Sigma Aldrich UK (sequences found in Supplementary Table 1) ...
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bioRxiv - Immunology 2021Quote: ... 20-mer gRNA sequences were introduced into primers (Millipore Sigma) and after hybridization were cloned into Esp3I-digested lentiGuide-puro using the NEBuilder® HiFi DNA Assembly Master mix (E2621 ...
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bioRxiv - Bioengineering 2022Quote: ... Cat# N0447] and primers (all Sigma-Aldrich, Supplementary Table 2) and were performed strictly following previously published protocols.35
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bioRxiv - Microbiology 2022Quote: ... DNA oligo primers were obtained from Sigma-Aldrich (Table S4). PCR products were purified using the PureLink PCR purification kit (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... and standard desalted p5 and p7 primers (Sigma, Table S1), containing inline-barcodes for multiplexing and partial adapters for Illumina sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... The recommended primers and PCR protocols designed by Jackson laboratories were used to verify the transgenes (primers purchased from Sigma). Following PCR amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... and primer removal using the SeqPlex RNA Amplification Kit (Sigma) yielding 150 to 400 nucleotide cDNA fragments ...
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bioRxiv - Microbiology 2023Quote: Primers used in this study were purchased from Sigma-Aldrich and are listed in Table S1 ...
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bioRxiv - Biochemistry 2023Quote: ... The primers were designed using SnapGene and synthesized by Sigma. The mutations were verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Primers were acquired from Sigma Aldrich (St. Louis, MO, USA).
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bioRxiv - Bioengineering 2023Quote: ... Gene-specific pre-designed oligonucleotide primers were purchase from Sigma. qRT-PCR was done using SsoAdvanced Universal SYBR Green Supermix and SFX96 Touch real time PCR detection system (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Primers used for each target gene were purchased from Sigma and are listed in Table S3
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bioRxiv - Molecular Biology 2024Quote: ... 10 pmol probe (IDT) and 10 pmol NDP1 primer (Sigma) (see sequences in Table S4 ...
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bioRxiv - Microbiology 2021Quote: ... using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche, Sigma-Aldrich, St. Louis, MO, USA). Samples were multiplexed using Takara long amplicon Taq polymerase with GC buffers following the manufacturer’s instructions for PCR barcoding for nanopore sequencing (EXP-PBC096 Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 5 minutes at RT with Giemsa (Sigma) diluted 1:1 in deionized water ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 5 minutes at RT with Giemsa (Sigma) diluted 1:1 in deionized water ...
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bioRxiv - Pathology 2022Quote: ... permeabilized (0.1% Triton X-100, Sigma, in PBS, 20min., RT), blocked with 2% goat serum (Life technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted using RNAzol®RT (Sigma-Aldrich) and 2 µg of total RNA was subjected to reverse transcription using qScript cDNA Synthesis Kit (Quanta) ...
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bioRxiv - Cell Biology 2020Quote: ... the TRAPEZE® RT Telomerase Detection Kit (Chemicon; EMD Millipore) was utilized ...
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bioRxiv - Developmental Biology 2021Quote: ... or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07; Sigma). Gli1 (Wen et al. ...
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bioRxiv - Cell Biology 2022Quote: ... RT and alkylated using 5.5 mM of iodoacetamide (Sigma-Aldrich) for 45 min ...
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bioRxiv - Immunology 2022Quote: ... fixed cells were blocked at RT with 1% BSA (Sigma) in PBS for 30 min and permeabilized with 0.1% Triton-X100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 min at RT and 70% isopropanol (Sigma-Aldrich) was added for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated for 1h at RT and DAPI (Sigma-Aldrich) was added for 10min to counterstain nuclei ...
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bioRxiv - Genetics 2023Quote: ... and 1 volume of chloroform (2 min, RT, Sigma Aldrich), the aqueous phase was recovered after centrifugation at 12 000 g ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was isolated using RNAzol®RT (Sigma- Aldrich) as per manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... After on-column DNase treatment with the DNase I Digest Set (Sigma-Aldrich, Taufkirchen, Germany) the RNA was quantified ...