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Citations for Millipore Sigma :
551 - 600 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Primers were ordered from Sigma-Aldrich (25nmole scale, desalted) and contained Biotin moiety on the 5’ ends for repair donor synthesis ...
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bioRxiv - Biochemistry 2021Quote: ... Primers were ordered from Genosys (Sigma-Aldirch, Burlington, MA) or Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2020Quote: ... Primer sequences (purchased from Sigma or Metabion internacional AG) were checked with BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) ...
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bioRxiv - Microbiology 2020Quote: ... and desalted or PAGE-purified oligonucleotide primers (Sigma Aldrich) by following manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... The list of qPCR primers (from Sigma-Aldrich, USA) is shown in Table 2 in Supplementary Material.
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bioRxiv - Neuroscience 2020Quote: Primers were ordered from Sigma-Aldrich (Castle Hill, NSW). Optimal primer dilutions and amplification efficiencies had previously been determined by TYP and AKS ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was performed using primers specific for actin (Sigma) and SRY (Thermofisher) ...
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bioRxiv - Molecular Biology 2022Quote: Primers used in this study were synthesized by Sigma/Merck ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: ... These standards primers were HPLC purified (Sigma Aldrich, US) and resuspended to a 100μM stock in molecular grade water ...
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bioRxiv - Cancer Biology 2022Quote: ... with random hexamer or oligo(dT) primers (Sigma-Aldrich). The cDNA samples were subjected to qPCR as described previously (41 ...
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bioRxiv - Pathology 2022Quote: ... and 1 μL of 5 μM primer stock (Sigma, see Supplementary table 1 for sequences ...
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bioRxiv - Biochemistry 2021Quote: ... All primers and oligonucleotides were obtained (Sigma, Gillingham UK) as salt free purified lyophilized DNA ...
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bioRxiv - Molecular Biology 2020Quote: dPCR primers and modified dPCR probes (Sigma Aldrich, Australia) were used to detect mutation g428c on a magnetic induction cycler (MIC ...
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bioRxiv - Neuroscience 2021Quote: ... The reverse primer harbors a 3xFLAG sequence (Sigma-Aldrich) to allow the expression analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... forward and reverse primers (Sigma; 100-500 nM each) and 1× PerfeCTa® SYBR® Green FastMix (Quantabio ...
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bioRxiv - Microbiology 2022Quote: The sequences of all primers (Sigma or Eurofins Genomics) used in this study are shown in Table S1.
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bioRxiv - Molecular Biology 2019Quote: The qPCR primers were synthesized by Sigma-Aldrich (USA) and the list of primers used in this study is provided in Supplementary Tables 1 and 2.
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bioRxiv - Genomics 2020Quote: ... Gene-specific primers were designed and purchased from Sigma. Primer sequences are detailed in S10 Table ...
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bioRxiv - Neuroscience 2020Quote: ... Primers and probes were finally synthesized by Sigma-Aldrich. All primers were tested on 100 ng of genomic DNA in order to validate experimentally their specificity ...
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bioRxiv - Plant Biology 2020Quote: ... lycopersicum oligonucleotide primers (Table S4) purchased from Sigma-Aldrich. Gene primers and probes were designed using Universal Probe Library (UPL ...
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bioRxiv - Molecular Biology 2019Quote: ... forward and reverse primer (Sigma; 100-500 nM each) and 25 ng of template were used ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: Primers used in the study (from IDT or Sigma). gRNA target sequence underlined.
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bioRxiv - Biochemistry 2019Quote: ... The Gibson assembly primers were purchased from Sigma-Aldrich. Mutated MvaT derivatives were generated using the same approach ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The biotin-bound capture primers (Sigma-Aldrich, Gillingham, UK) were attached to biotinylated magnetic beads (Promega ...
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bioRxiv - Immunology 2020Quote: ... The following primers were purchased for use from Sigma: mAqp9 fwd 5′-TGGTGTCTACCATGTTCCTCC-3′ ...
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bioRxiv - Developmental Biology 2022Quote: ... with 200 nM Forward and Reverse primers (Sigma-Aldrich; designed using Primer3 software (Untergasser et al. ...
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bioRxiv - Physiology 2022Quote: ... 1.2 µL of KiCqStart SYBR Green Predesigned Primers (Sigma) at a concentration of 10 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... Primers were from Sigma-Aldrich (St. Louis, MO, USA) and were directed against exon-exon boundaries for RT-qPCR or directed against intron-exon boundaries or introns for DNA-qPCR (Table S1) ...
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bioRxiv - Bioengineering 2023Quote: The primers (M1-M4) were obtained from Sigma Aldrich and the sequence is mentioned in Table 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... and KiCqStart SYBR green primers (Sigma Merck, Table S1). Obtained Ct values were used for relative quantification of mRNA expression using the ddCt method ...
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bioRxiv - Immunology 2023Quote: ... All other primers were designed and obtained from Sigma and are summarized in Supplemental Tables 2 and 3 ...
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bioRxiv - Biophysics 2023Quote: ... 0.6 μL GAPDH and β-actin primers (Sigma Aldrich), 6.4 μL H2O and 5 μL cDNA ...
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bioRxiv - Microbiology 2024Quote: ... Oligonucleotide primers (Table S7) were synthesized by Sigma-Aldrich. Vector pDS132 was used to construct plasmids for in-frame deletion ...
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bioRxiv - Immunology 2023Quote: ... and primers supplied by Sigma Aldrich (see table 1) were used unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... and random hexamer primers (Sigma-Aldrich, cat.no. 11034731001-2MG). Gene expression was determined by qPCR using InnuMIX qPCR DSGreen Standard (Analytik Jena ...
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bioRxiv - Cell Biology 2024Quote: ... and specifically designed primers (Eurogentec, Liège, Belgium; Millipore Sigma) listed in Table 1 were used ...
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bioRxiv - Cell Biology 2020Quote: ... miRNA-147-3p or miR-298-5p mimics (Dharmacon) and incubated for 72 hours in DMEM/OptiMEM medium (Sigma-Aldrich/Thermo Fisher Scientific) prior lysis.
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bioRxiv - Cancer Biology 2019Quote: ... for primary cells miR-E shRNA viruses were concentrated to increase transduction efficiency using Centriprep Centrifugal filter YM-50 (Millipore, Billerica, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using SYBR Green Mastermix (Sigma) and primers detailed in Table 3.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked at RT with 5% BSA (Sigma) dissolved into PBS ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL ThermaSTOP RT (Sigma Aldrich, #TSTOPRT-250). Mix 1 was heated to 65°C for 5 min and then snap-chilled on ice for at least 2 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Hoechst 33342 (1μg/mL; 5 min; RT; Sigma).
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated by using RNAZol-RT reagent (Sigma) from exponentially growing MtbH37Rv/Msm mc2155 and its Rho CKD strain ...
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bioRxiv - Developmental Biology 2023Quote: ... overnight at RT and included in paraffin (Sigma-Aldrich) following standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... at RT and fixed with 4% paraformaldehyde (Sigma Aldrich) in CB for 20 min (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... Followed by an incubation in darkens with SIGMA FAST 3,3’-Diaminobenzidine Tablet SETS (SIGMA) for 75 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... a set of CasMam oligonucleotides was designed and synthesized (Sigma-Aldrich/Merck, desalt purification). The oligonucleotides contained the optimized linker sequences (α/β/γ/δ/ε/ω ...
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bioRxiv - Plant Biology 2020Quote: ... After removal of DNA using the on-column DNase digestion Set (Sigma-Aldrich Australia), RNA quality and quantity was assessed on a TapeStation 2200 system (Agilent) ...
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bioRxiv - Genomics 2019Quote: ... frozen tissues were placed into a 2 ml KIMBLE Dounce tissue grinder set (Sigma) with homogenization buffer consisted of 20 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... A standard set of phosphopeptides (4 pmol/peptide sample, MS PhosphoMix1 Light, Sigma, MSPL1) were spiked into each sample ...