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Citations for Millipore Sigma :
451 - 500 of 7689 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21 and purified by glutathione-agarose affinity chromatography (Sigma-Aldrich Cat #G4510) and eluted using elution buffer (50mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... NF54/iGP2 strain was additionally supplemented with 2.5 mM D-(+)-glucosamine hydrochloride (Sigma #1514) for maintenance ...
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bioRxiv - Biochemistry 2019Quote: ... coli strains were grown at 37°C and cultured in LB medium (L3522, Sigma), supplemented with the indicated concentration of kanamycin (30 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: Transformation of Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) were made with a pET3d-His6-CrFBA3 plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... smegmatis mc2 155 strain and its mutants were propagated in liquid 7H9 medium (Sigma) with 10% ADC supplement (5% BSA ...
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bioRxiv - Microbiology 2022Quote: ... Luria Bertani broth and agar were used to maintain bacterial strains (Sigma, Dorset, UK). Strains were sub-cultured in Mueller Hinton Broth II (MHBII ...
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bioRxiv - Microbiology 2023Quote: ... baumannii strains 19606 and 398 was measured using the Ammonia Assay Kit (AA0100, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Mycobacterium smegmatis mc2155 and derivative strains were grown in Middlebrook 7H9 liquid media (Millipore) supplemented with ADC (5 g l-1 bovine albumin fraction V ...
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bioRxiv - Microbiology 2022Quote: ... aureus strains were grown in Tryptic Soy Broth (TSB, ref. 22092 by Merck-Millipore), B ...
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bioRxiv - Microbiology 2023Quote: ... Typhimurium strains were induced for recombinase expression using 20 mM L-arabinose (Sigma, USA). Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The genotype of this strain (Supplementary Table S2) is similar to DH5α (Sigma Aldrich commercial information ...
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bioRxiv - Microbiology 2023Quote: ... the parasite strains were selected with 25 μg/ml mycophenolic acid (MPA) (Millipore 89287) and 25 μg/ml xanthine (Xan ...
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bioRxiv - Microbiology 2024Quote: ... The subcultures of strains carrying pBAD33 plasmids were grown with 0.2% arabinose (Sigma-Aldrich). OD600 was measured ...
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bioRxiv - Biochemistry 2023Quote: ... capsulatus deletion strains were grown with 20 μg/ml streptomycin sulfate (Sigma, Darmstadt, Germany) and R ...
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bioRxiv - Microbiology 2024Quote: ... coli strain MG1655 on agar plates containing 0.3 mM diaminopimelic acid (DAP; Sigma-Aldrich). To induce expression from the PBAD promoter ...
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bioRxiv - Microbiology 2024Quote: ... Antibiotics used to select transformants during strain construction were cefotaxime (10 mg/L, Sigma) and kanamycin (50 mg/L ...
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bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...
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bioRxiv - Cell Biology 2019Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2µg/ml Puromycin (Sigma-Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2020Quote: ... Airway Chips infected with influenza virus (MOI = 0.1) were treated with 1 µM oseltamivir acid (Sigma-Aldrich) under flow (60 µl/h ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45μm filter (EMD Millipore) and used for transduction of MCF-10A cells in the presence of polybrene (8μg/mL) ...
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bioRxiv - Neuroscience 2021Quote: Each animal (with DREADD virus injection) received CLZ (0.5 mg/kg, Sigma-Aldrich, dissolved with 0.1% DMSO) or vehicle (sterilized saline with 0.1% DMSO ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... pLVX-EF1α-mKate2-WPRE-Neo virus supplemented with 10 µg/ml polybrene infection/transfection reagent (Sigma-Aldrich) was incubated with PSCs grown in monolayer for 24 h ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
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bioRxiv - Microbiology 2021Quote: ... and 10% formamide in DEPC-treated H2O) with anti-influenza A virus NP antibody (Millipore, 1:2,000) and FISH probes ...
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bioRxiv - Bioengineering 2021Quote: ... cells were transduced with 4 µL library virus/well along with 8 µg/mL Polybrene (Sigma-Aldrich) aiming for an MOI at 0.3-0.4 (Supplementary Methods and Results) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultivated with the virus in the presence of 4 μg/ml polybrene (Sigma-Aldrich) for 12 hr.
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with virus (MOI 0.5-1.0) and 5 µg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 250 µg/mL G418 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Virus was then removed and cells were overlayed with 3 mL of DMEM with 0.8% methylcellulose (Sigma), 1% FBS ...
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bioRxiv - Physiology 2020Quote: Virus carrying the gene of interest was used to infect cell lines with 10mg/mL polybrene (Millipore). Media was replaced after 24 h and cells were sorted for GFP positivity after 48-72 h post-infection ...
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bioRxiv - Genetics 2019Quote: ... The resulting myoblasts were then infected with a retroviral cocktail containing Myf6-CTAP virus and polybrene (Millipore) for 8 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Suspensions of L-cells (from ATCC) for virus production were maintained in Joklik MEM medium (Sigma-Aldrich) supplemented with 1% L-Glutamine (Gibco.) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore) filter ...
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...